Chromosomes Behavior at Meiosis in Chlorophytum stenopetalum Bak at Pachytene, Diakinesis and Diplotene

Meiotic prophase is classically subdivided into five stages: leptotene, zygotene, pachytene, diplotene, and diakinesis. The objective of this paper is to evaluate the behaviours of Chromosomes in Chlorophytum stenopetalum Bak.at pachytene /diakinesis and metaphase. The flower buds at right age were harvested, fixed in Cornoy’s solution (3 part of absolute alcohol and 1 part of acetic alcohol) and preserved in a refrigerator at -4°𝐶 for at least thirty minutes. The flower buds were then hydrolyzed in 10% HCl for 3-5 minutes. Prepared slides were viewed using an Armscope microscope equipped with digital automatic camera. At diakinesis seven bivalents (7 II) were predominantly observed (74.2%). Chromosomal stickiness is quite evidence. In addition, cross configuration and its resultant, ring formation at metaphase indicate the presence of translocation heterozgosity in the chromosomes of the species investigated. These abnormalities are likely to affect microsporogenesis and pollen viability.

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Meiotic Chromosomes Behavious in Chlorophytum stenopetalum Bak

10.37179/rijpse.000001 ◽

2020 ◽

Vol 01 (01) ◽

pp. 001-003

Author(s):

Omokanye SB

Keyword(s):

Pollen Viability ◽

Ring Formation ◽

Absolute Alcohol ◽

Meiotic Chromosomes ◽

Acetic Alcohol

Chromosomes behavior at meiosis in Chlorophytum stenopetalum Bak. was investigated especially at pachytene /diakinesis and metaphase. The 􀏐lower buds at right age were harvested, 􀏐ixed in Cornoy’s solution (3 part of absolute alcohol and 1 part of acetic alcohol) and preserved in a refrigerator at -4 for at least thirty minutes. The 􀏐lower buds were then hydrolyzed in 10% HCl for 3-5 minutes. Prepared slides were viewed using an Armscope microscope equipped with digital automatic camera. At diakinesis seven bivalents (7 II) were predominantly observed (74.2%). Chromosomal stickiness is quite evidence. In addition, cross con􀏐iguration and its resultant, ring formation at metaphase indicate the presence of translocation heterozgosity in the chromosomes of the species investigated. These abnormalities are likely to affect microsporogenesis and pollen viability.

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Floral Biology and Pollen Viability of Passiflora edulis Sims

Journal of Experimental Agriculture International ◽

10.9734/jeai/2019/v41i130386 ◽

2019 ◽

pp. 1-9

Author(s):

Ivan Sérgio da Silva Oliveira ◽

Vênia Camelo de Souza ◽

Sara Beatriz da Costa Santos ◽

Jazielly Nascimento da Rocha Almeida ◽

Josinaldo da Silva Henrique ◽

...

Keyword(s):

Pollen Viability ◽

Pollen Grains ◽

Floral Biology ◽

Passion Fruit ◽

Average Height ◽

Flower Buds ◽

Passiflora Edulis ◽

Tropical Fruit ◽

Passiflora Edulis Sims ◽

Yellow Passion Fruit

Yellow passion fruit is a native tropical fruit tree whose cultivation has evolved very rapidly in Brazil; therefore, studies on the ecology of reproduction are needed. The study of pollen viability is an important tool to observe the male potential of the species. The objective of this study was to estimate pollen viability, pollen/ovule ratio and floral biology of Passiflora edulis Sims in organic cultivation located in Bananeiras, PB, and Brazilian. The study was developed in a completely randomized design, using ten flower buds in the pre-anthesis. The analyses performed in the biology laboratory of the Center of Human, Social and Agrarian Sciences of the Federal University of Paraiba. For the pollen viability analysis, all the anthers of the ten flower buds were used, five anthers per flower, which were crushed on a glass slide and with the aid of Alexander dye, the purple pollen grains were considered viable and the green ones unviable. To measure flower morphology and biometrics, a digital pachymeter, analytical balance and ruler were used. Five stamens form the androecium; the fillets are of short length inserted below the ovary. The flowers have three stigmas with an average height of 16.5mm, an average of 32,136 viable pollens, 58 unviable pollen grains and 140 ovules per ovary, having a pollen viability of 99.8% and a pollen/ovule ratio of approximately 95/1 pollen grains per ovule. Their structures are uniform, with no biometric variations in the amount of petals, sepals, anthers and stigma length. Alexander's reactive dye was effective in staining the pollen grains of yellow passion fruit. The pollen/ovule ratio indicates that this species performs facultative autogamy as well; however, this classification alone is not sufficient to affirm its reproductive system.

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Pollen viability and floral biology of Mandacaru (Cereus jamacaru (DC) (Cactaceae))

Research Society and Development ◽

10.33448/rsd-v9i8.6671 ◽

2020 ◽

Vol 9 (8) ◽

pp. e997986671

Author(s):

Paulo Marks de Araújo Costa ◽

Vênia Camelo de Souza ◽

Ivan Sérgio da Silva Oliveira ◽

Vagner Sousa da Costa ◽

Emerson Serafim Barros

Keyword(s):

Pollen Viability ◽

Floral Biology ◽

Glass Slide ◽

Morphological Identification ◽

Flower Buds ◽

Flower Bud ◽

Natural Distribution ◽

Hermaphrodite Flower ◽

Large Size ◽

Native Tree Species

Pollen fertility is indicated by viability, which influences the reproductive success of plants. Mandacaru is a native tree species with natural distribution in the Caatinga. The objective was to verify the pollen viability, pollen per ovule ratio and the morphological identification of the mandacaru flower (Cereus jamacaru). Ten flower buds were used and one hundred anthers of these buds, which and stained with Alexander dye on checkered glass slide and viable and non-viable pollens were accounted amount of ovule per flower bud was counted the pollen/ovule ratio. Mandacaru presented viability of 99.41% the pollen, with an average of 314,686 pollens on each flower. There were 356 pollens for each ovule, this indicates the species reproductive system for facultative autogamous and facultative xenogamics. The flower is hermaphrodite, with gynoecium measuring an average of 160.9 mm, an average of 883 ovules per flower, the androecium averaging 577 anthers per flower, 545 pollen per anther. Mandacaru has high pollen viability, with large size hermaphrodite flower, with high number of anthers and pollen contained in these anthers.

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Reproductive Traits of Hermaphroditic Muscadine Cultivars

HortScience ◽

10.21273/hortsci.51.3.255 ◽

2016 ◽

Vol 51 (3) ◽

pp. 255-261 ◽

Cited By ~ 1

Author(s):

Luiz A. Biasi ◽

Patrick J. Conner

Keyword(s):

Pollen Germination ◽

Pollen Viability ◽

Calcium Nitrate ◽

Pollen Grains ◽

Reproductive Traits ◽

Pollen Grain ◽

Flowering Period ◽

Flower Buds ◽

Vitis Rotundifolia ◽

Ethanesulfonic Acid

Native muscadines (Vitis rotundifolia Michx.) are dioecious, but muscadine vineyards are usually planted with a mixture of female plants and hermaphroditic pollenizers. Hermaphroditic cultivars are derived from either of two separate sources original hermaphroditic plants, H1 or H2. Nine hermaphroditic and two female cultivars were studied to determine their potential as pollenizers. Inflorescences of each cultivar were collected in the field to evaluate the number of anthers per flower, the number of pollen grains per anther, and pollen viability and germination in the main and secondary flowering periods. The number of flower clusters per shoot ranged from one to four with most producing two or three flower clusters per shoot. The number of anthers per flower varied by cultivar and cluster position, but in general was between six and eight anthers per flower. ‘Noble’ showed the highest pollen grains production per anther and per flower, reaching 5777 and 39,860, respectively, in the first cluster and ‘Carlos’ produced the least amount of pollen. All cultivars that had secondary flowering showed lower pollen production per anther and per flower as compared with the main flowering period. Optimal muscadine pollen germination media contained 50 mg·L−1 boric acid, 145 mg·L−1 calcium nitrate, 188 g·L−1 sucrose, 10 g·L−1 agar, and 10 mm 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.0. The pollen grain viability of hermaphroditic and female cultivars was high, but pollen grain germination was low in hermaphroditic cultivars and absent in female cultivars. H1-derived cultivars produced more flower buds per cluster and higher germination rates than H2-derived cultivars, indicating they may be better pollenizers. Chemical names used: 2-(N-morpholino)ethanesulfonic acid (MES).

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Purification of an antifungal PR-5 protein from flower buds of Brassica campestris and cloning of its gene

Physiologia Plantarum ◽

10.1034/j.1399-3054.1997.1010319.x ◽

1997 ◽

Vol 101 (3) ◽

pp. 583-590 ◽

Cited By ~ 1

Author(s):

Na Eun Cheong ◽

Yeon Ok Choi ◽

Woe Yeon Kim ◽

Sun Chang Kim ◽

Moo Je Cho ◽

...

Keyword(s):

Brassica Campestris ◽

Flower Buds ◽

Its Gene

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Butenolide ring formation in cardenolide biosynthesis: Spontaneous cyclization of pregnane-21-O-malonyl hemiesters

Planta Medica ◽

10.1055/s-2007-987133 ◽

2007 ◽

Vol 73 (09) ◽

Author(s):

RM Pádua ◽

R Waibel ◽

SP Kuate ◽

PK Riedl ◽

P Gmeiner ◽

...

Keyword(s):

Ring Formation

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A New Species of Eugenia subg. Pseudeugenia (Myrtaceae, Myrteae) from Brazilian Atlantic Forest

Systematic Botany ◽

10.1600/036364420x15935294613518 ◽

2020 ◽

Vol 45 (3) ◽

pp. 537-543

Author(s):

Karinne Sampaio Valdemarin ◽

Jair Eustáquio Quintino Faria ◽

Fiorella Fernanda Mazine ◽

Vinicius Castro Souza

Keyword(s):

New Species ◽

Atlantic Forest ◽

Conservation Status ◽

Forest Vegetation ◽

Vegetation Types ◽

Flower Buds ◽

Diagnostic Features ◽

Closely Related Species ◽

Lowland Forests ◽

A New Species

Abstract—A new species of Eugenia from the Atlantic forest of Brazil is described and illustrated. Eugenia flavicarpa is restricted to the Floresta de Tabuleiro (lowland forests) of Espírito Santo state and is nested in Eugenia subg. Pseudeugenia. Considering all other species of the subgenus that occur in forest vegetation types of the Atlantic forest phytogeographic domain, Eugenia flavicarpa can be distinguished mainly by the combination of smooth leaves with indumentum on both surfaces, with two marginal veins, usually ramiflorous inflorescences, pedicels 4.5‐9.7 mm long, flower buds 3.5‐4 mm in diameter, and by the calyx lobes that are 2‐3 mm long with rounded to obtuse apices. Morphological analyses were performed to explore the significance of quantitative diagnostic features between the new species and the closely related species, Eugenia farneyi. Notes on the habitat, distribution, phenology, and conservation status of Eugenia flavicarpa are provided, as well as a key for all species of Eugenia subg. Pseudeugenia from forest vegetation of the Atlantic forest phytogeographic domain.

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