Metabolic Activation and Inactivation of Metanil Yellow and Orange II in the Salmonella typhimurium his− Reversion Assay

1996 ◽  
Vol 59 (3) ◽  
pp. 244-248 ◽  
Author(s):  
P. B. RASTOGI ◽  
R. E. LEVIN
Keyword(s):  
Salmonella Typhimurium ◽  
Rat Liver ◽  
Metabolic Activation ◽  
Flavin Mononucleotide ◽  
Orange Ii ◽  
Combined Use ◽  
Mutagenic Response ◽  
Experimental Protocols ◽  
Metanil Yellow ◽  
S9 Fraction

Among a variety of experimental protocols used, the combined use of 0.5% dextrose in bottom agar and 1 μmol of flavin mononucleotide (FMN) in preincubation mixtures without fraction S9 mix resulted in the highest level of induced Salmonella typhimurium his− reversions with both dyes metanil yellow and orange II with strain TA100. Strain TA98 yielded notably lower levels of reversions under the same conditions. The presence of uninduced hamster liver S9 fraction resulted in a weak mutagenic response while Aroclor 1254-induced rat liver S9 fraction resulted in the complete absence of mutagenicity with both strains and with both dyes.

scholarly journals Evaluation of mutagenic activity of the pesticides: actara, sencor, mospilan, pencozeb, fastac in the Ames test

2008 ◽  
Vol 6 (4) ◽  
pp. 29-33 ◽  
Author(s):  
Nazira S Karamova ◽  
Alexandra P Denisova ◽  
Zenon Stasevski
Keyword(s):  
Salmonella Typhimurium ◽  
Rat Liver ◽  
Potato Plant ◽  
Ames Test ◽  
Mutagenic Activity ◽  
Metabolic Activation ◽  
Mutagenic Effect ◽  
Toxic Concentrations ◽  
S9 Fraction

The mutagenic activity of five pesticides actara, sencor, mospilan, pencozeb, fastac widely used for treatment of potato plant lands in Tatarstan was tested in the Ames test. The non toxic concentrations of the pesticides determined in preliminary cytotoxicty test were used in the Ames assay. Pesticides actara, mospilan, pencozeb, fastac did not show mutagenic effect in Salmonella typhimurium TA 100 without rat liver S9 fraction. The weak mutagenic effect of herbicide sencor was established at concentration 1 ug/plate. Metabolic activation in vitro using rat liver S9 fraction decreased the mutagenic activity of sencor and did not alter the mutagenicity rate of the pesticides actara, mospilan, pencozeb and fastac.

scholarly journals Metabolic Activation of Bisphenol A by Rat Liver S9 Fraction

2001 ◽  
Vol 62 (2) ◽  
pp. 221-227 ◽  
Author(s):  
S.'i. Yoshihara
Keyword(s):  
Bisphenol A ◽  
Rat Liver ◽  
Metabolic Activation ◽  
S9 Fraction

scholarly journals CL 64, 855, a potent do anti-Trypanosoma cruzi drug, is also mutagenic in the salmonella/microsome assay

1986 ◽  
Vol 81 (1) ◽  
pp. 49-52 ◽  
Author(s):  
R. C. C. Ferreira ◽  
L. C. S. Ferreira
Keyword(s):  
Trypanosoma Cruzi ◽  
Salmonella Typhimurium ◽  
Rat Liver ◽  
Metabolic Activation ◽  
Mutagenic Action ◽  
Short Term ◽  
Mutagenicity Test

The nitroimidazole-tiadiazole derivative CL 64,855 (2-amino-5-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazole, a potent anti-trypanosomal drug, was assayed in a short-term bacterial mutagenicity test with Salmonella typhimurium strains TA 98, TA 100 and TA 102. Results indicate that CL 64,855 is a potent frameshift mutagen detected by strains TA 98 and TA 102. CL 64,855 was able to revert the indicators strains at concentrations as low as 0.1 µg/plate. Metabolic activation experiments with rat liver microsomal fractions did not increase the mutagenic action of Cl 64,855.

Mutagenicity of Bisbenzimidazole Derivatives

2010 ◽  
Vol 65 (1-2) ◽  
pp. 10-14 ◽  
Author(s):  
Filiz Susuz Alanyalı ◽  
Merve Arıcı ◽  
Öge Artagan ◽  
İlhan Işıkdağ ◽  
Yusuf Özkay
Keyword(s):  
Salmonella Typhimurium ◽  
Base Pair ◽  
Frameshift Mutation ◽  
Metabolic Activation ◽  
Mutagenic Response ◽  
Base Pair Substitution ◽  
Incorporation Assay ◽  
Substitution Mutation

The mutagenicities of 2,2’-(di-3-hydroxyphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-(di- 4-hy droxyphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-(di-3-methoxyphenyl)-1H,1H’-[5,5’]- bisbenzimidazole, 2,2’-bis-(4-nitrophenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-bis-(3-nitrophenyl)- 1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-bis-(4-methylphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-(di-4-methoxyphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, and 2,2’-bis-(3-me thylphenyl)- 1H,1H’-[5,5’]-bisbenzimidazole were studied in vitro using two strains of Salmonella typhimurium with frameshift mutation (TA98) and base-pair substitution mutation (TA100) as the plate incorporation assay in the absence of metabolic activation. These compounds are currently used to treat cancer. 4-Nitrophenyl and 3-nitrophenyl compounds were found to be mutagenic on both strains of Salmonella. A clear mutagenic response was seen in nitro-bound derivatives. The mutagenic response in Salmonella test strains (TA98, TA100) and structures of molecules suggest that nitro-bound molecules could be mutagenic.

scholarly journals Mutagenic activity of airborne particulate matter from the urban area of Porto Alegre, Brazil

1998 ◽  
Vol 21 (2) ◽  
pp. 247-253 ◽  
Author(s):  
Vera Maria Ferrão Vargas ◽  
Rubem Cesar Horn ◽  
Régis Rolim Guidobono ◽  
Ana Beatriz Mittelstaedt ◽  
Irascema Girardi de Azevedo
Keyword(s):  
Particulate Matter ◽  
Salmonella Typhimurium ◽  
Urban Area ◽  
Mutagenic Activity ◽  
Airborne Particulate Matter ◽  
Metabolic Activation ◽  
Polar Fraction ◽  
Airborne Particulate ◽  
Porto Alegre ◽  
Mutagenic Response

The mutagenic activity of airborne particulate matter collected from three different sites within the urban area of Porto Alegre, Brazil, was investigated using a Salmonella/microsome assay. Samples were extracted by sonication, sequentially, with cyclohexane (CX), and dichloromethane (DCM), for a rough fractionation by polarity. The different fractions were tested for mutagenicity using Salmonella typhimurium strains TA98, with and without metabolic activation (S9 mix fraction), and TA98NR and TA98/1,8-DNP6, without metabolic activation. Mutagenic response was observed for frameshift strain TA98 in assays with and without metabolization for two sites (sites 2 and 3), which had considerable risk of environmental contamination by nonpolar (CX) and/or moderately polar (DCM) compounds. However, the values of revertants/m3 (rev/m3) were highest on the site subject to automobile exhaust (site 3) in assays without (9.56 rev/m3) and with metabolization (5.08 rev/m3). Maximum mutagenic activity was detected in the moderately polar fraction, decreasing after metabolization. Nevertheless, the nonpolar fractions (CX) gave higher mutagenic activity in the presence of metabolization than in the absence of the S9 mix fraction. The responses observed for TA98NR and TA98/1,8-DNP6 strains suggest the activity of nitrocompounds.

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