Flow Cytometric Analysis of Cells Obtained from Human Bone Marrow Cultures

2020 ◽  
pp. 121-146
Author(s):  
David Brott ◽  
Manfred R. Koller ◽  
Sue A. Rummel ◽  
Bernhard Palsson
Keyword(s):  
Bone Marrow ◽  
Flow Cytometric Analysis ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Flow Cytometric ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

Formation of Multinucleated Cells that Respond to Osteotropic Hormones in Long Term Human Bone Marrow Cultures*

Endocrinology ◽  
1987 ◽  
Vol 120 (6) ◽  
pp. 2326-2333 ◽  
Author(s):  
B. R. MACDONALD ◽  
N. TAKAHASHI ◽  
L. M. MCMANUS ◽  
J. HOLAHAN, ◽  
G. R. MUNDY ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Multinucleated Cells ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

scholarly journals Phospholipase C release of basic fibroblast growth factor from human bone marrow cultures as a biologically active complex with a phosphatidylinositol-anchored heparan sulfate proteoglycan.

1991 ◽  
Vol 114 (6) ◽  
pp. 1275-1283 ◽  
Author(s):  
G Brunner ◽  
J Gabrilove ◽  
D B Rifkin ◽  
E L Wilson
Keyword(s):  
Bone Marrow ◽  
Cell Surface ◽  
Human Bone ◽  
Biologically Active ◽  
Human Bone Marrow ◽  
Sulfate Proteoglycan ◽  
Fibroblast Growth ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

Basic fibroblast growth factor (bFGF) is a potent mitogen for human bone marrow stromal cells and stimulates haematopoiesis in vitro. We report here that primary human bone marrow cultures contain bFGF and express heparin-like bFGF binding sites on the cell surface and in the extracellular matrix (ECM). bFGF bound predominantly to a 200-kD cell surface heparan sulfate proteoglycan (HSPG), which was also found in conditioned medium. bFGF was released from bone marrow cultures by incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) and, less efficiently, by plasmin. Solubilized bFGF was found as a complex with the 200-kD HSPG. The complex was biologically active as shown by its ability to stimulate plasminogen activator production in bovine aortic endothelial cells. bFGF-HSPG complexes of bovine endothelial cells, however, were not released by PI-PLC. While only trace amounts of the bFGF-binding 200-kD HSPG were released spontaneously from bone marrow cultures, incubation with PI-PLC solubilized almost all of the 200-kD HSPG. The HSPG could be metabolically labeled with ethanolamine or palmitate, which was partially removed by treatment with PI-PLC. These findings indicate linkage of the HSPG to the cell surface via a phosphatidylinositol anchor. Plasmin released the 200-kD HSPG less efficiently than PI-PLC. We conclude that HSPGs of human bone marrow serve as a reservoir for bFGF, from which it can be released in a biologically active form via a dual mechanism; one involving a putative endogenous phospholipase, the other involving the proteolytic cascade of plasminogen activation.

Macrophage inflammatory protein 1-alpha is a potential osteoclast stimulatory factor in multiple myeloma

Blood ◽  
2000 ◽  
Vol 96 (2) ◽  
pp. 671-675 ◽  
Author(s):  
Sun Jin Choi ◽  
Jose C. Cruz ◽  
Fiona Craig ◽  
Hoyeon Chung ◽  
Rowena D. Devlin ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Messenger Rna ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Stimulatory Factor ◽  
Normal Controls ◽  
Marrow Cultures

Abstract This study was designed to determine if macrophage inhibitory protein-1 (MIP-1), a recently described osteoclast (OCL) stimulatory factor,1 was present in marrow from patients with multiple myeloma (MM) and possibly involved in the bone destructive process. MIP-1, but not interleukin-1β (IL-1β), tumor necrosis factor-β (TNF-β), or interleukin-6 (IL-6), messenger RNA was elevated in freshly isolated bone marrow from 3 of 4 patients with MM compared to normal controls. Furthermore, enzyme-linked immunosorbent assays of freshly isolated bone marrow plasma detected increased concentrations of hMIP-1 (range, 75-7784 pg/mL) in 8 of 13 patients (62%) with active myeloma, in 3 of 18 patients (17%) with stable myeloma (range, 75-190.3), as well as in conditioned media from 4 of 5 lymphoblastoid cell lines (LCLs) derived from patients with MM. Mildly elevated levels of MIP-1 were detected in 3 of 14 patients (21%) with other hematologic diagnoses (range, 80.2-118.3, median value of 96 pg/mL) but not in normal controls (0 of 7). MIP-1 was not detected in the peripheral blood of any patients with MM. In addition, recombinant hMIP-1 induced OCL formation in human bone marrow cultures. Importantly, addition of a neutralizing antibody to MIP-1 to human bone marrow cultures treated with freshly isolated marrow plasma from patients with MM blocked the increased OCL formation induced by these marrow samples but had no effect on control levels of OCL formation. Thus, high levels of MIP-1 are expressed in marrow samples from patients with MM, but not in marrow from patients with other hematologic disorders or controls, and support an important role for MIP-1 as one of the major factors responsible for the increased OCL stimulatory activity in patients with active MM.

Formation of osteoclasts and osteoblast-like cells in long-term human bone marrow cultures

Apmis ◽  
1991 ◽  
Vol 99 (1-6) ◽  
pp. 262-268 ◽  
Author(s):  
M. KASSEM ◽  
Li. MOSEKILDE ◽  
J. RUNGBY ◽  
Le. MOSEKILDE ◽  
F. MELSEN ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures
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