Development and Validation of UV Spectroscopic Method for Estimation of Albendazole In Tablet Dosage Form

Objective: The objective of the present study is to develop ultraviolet (UV)-spectroscopic method using pure drug and tablet dosage form that consistently produces a drug with a minimal variation that adheres to quality criteria of purity, identity, and potency. Methods: UV-spectrophotometric method has been developed using a solvent composed of methanol:water (30:70) as a diluent to determine the dalfampridine (DFP) content in bulk and pharmaceutical dosage form at predetermined λmax of 262 nm. Results: It was proved linear in the range of 02–12 μg/ml and exhibited a good correlation coefficient (r2 = 0.9915) and excellent mean recovery (0.004136347%). This method was successfully applied to the determination of DFP content of marketed tablet Dalstep 10 mg (Sun Pharmaceutical Pvt. Ltd.,) from India; the results were in good agreement with the label claims. Conclusion: The method proved to be simple, accurate, precise, specific, robust, and less time consuming and can be applied for the determination of DFP in bulk and marketed formulation.

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Method Development and Validation of Visible Spectroscopic Method for the Estimation of Paroxetine Hydrochloride in Pure and Tablet Dosage Form

Chemical Science Transactions ◽

10.7598/cst2017.1408 ◽

2017 ◽

Vol 6 (4) ◽

Keyword(s):

Dosage Form ◽

Method Development ◽

Spectroscopic Method ◽

Method Development And Validation ◽

Paroxetine Hydrochloride ◽

Development And Validation ◽

Tablet Dosage

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DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF LAMIVUDINE IN TABLET DOSAGE FORM

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017v9i6.23658 ◽

2017 ◽

Vol 9 (6) ◽

pp. 86

Author(s):

Kalpesh V. Sonar ◽

Prabodh Sapkale ◽

Anil Jadhav ◽

Tushar Deshmukh ◽

Swapnil Patil ◽

...

Keyword(s):

Quality Control ◽

Absorption Spectrum ◽

Melting Point ◽

Dosage Form ◽

Spectroscopic Method ◽

Beer’S Law ◽

Solubility Studies ◽

Development And Validation ◽

Tablet Dosage

Objective: To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Lamivudine in tablet dosage form.Methods: The drug is freely soluble in analytical grade water. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade water. The drug obeyed the Beer’s law and showed a good correlation of concentration with absorption which reflects in linearity. The UV spectroscopic method was developed for estimation of lamivudine in tablet dosage form and also validated as per ICH guidelines.Results: The drug is freely soluble in analytical grade water, slightly soluble in methanol and practically insoluble in acetone. So, the analytical grade water is used as a diluent in the method. The melting point of lamivudine was found to be 160-161˚C (uncorrected). It showed absorption maxima 268 nm in analytical grade water. On the basis of the absorption spectrum, the working concentration was set on 10µg/ml (PPM). The linearity was observed between 6-14 μg/ml (PPM). The results of the analysis were validated by recovery studies. The recovery was found to be 98.7, 101 and 99.2% for three levels respectively. The % RSD for precision was found to be 0.62%.Conclusion: A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Lamivudine in tablet dosage form. The method could be considered for the determination of Lamivudine in quality control laboratories.

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