scholarly journals DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF LAMIVUDINE IN TABLET DOSAGE FORM

2017 ◽  
Vol 9 (6) ◽  
pp. 86
Author(s):  
Kalpesh V. Sonar ◽  
Prabodh Sapkale ◽  
Anil Jadhav ◽  
Tushar Deshmukh ◽  
Swapnil Patil ◽  
...  
Keyword(s):  
Quality Control ◽  
Absorption Spectrum ◽  
Melting Point ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Beer’S Law ◽  
Solubility Studies ◽  
Development And Validation ◽  
Tablet Dosage

Objective: To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Lamivudine in tablet dosage form.Methods: The drug is freely soluble in analytical grade water. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade water. The drug obeyed the Beer’s law and showed a good correlation of concentration with absorption which reflects in linearity. The UV spectroscopic method was developed for estimation of lamivudine in tablet dosage form and also validated as per ICH guidelines.Results: The drug is freely soluble in analytical grade water, slightly soluble in methanol and practically insoluble in acetone. So, the analytical grade water is used as a diluent in the method. The melting point of lamivudine was found to be 160-161˚C (uncorrected). It showed absorption maxima 268 nm in analytical grade water. On the basis of the absorption spectrum, the working concentration was set on 10µg/ml (PPM). The linearity was observed between 6-14 μg/ml (PPM). The results of the analysis were validated by recovery studies. The recovery was found to be 98.7, 101 and 99.2% for three levels respectively. The % RSD for precision was found to be 0.62%.Conclusion: A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Lamivudine in tablet dosage form. The method could be considered for the determination of Lamivudine in quality control laboratories.

scholarly journals Development DEVELOPMENT AND VALIDATION OF NOVEL ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF DALFAMPRIDINE IN BULK AND IN PHARMACEUTICAL FORMULATION

2019 ◽  
pp. 275-279
Author(s):  
VAIBHAV S KHODKE ◽  
GAME MD
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Quality Criteria ◽  
Pharmaceutical Dosage Form ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Good Agreement

Objective: The objective of the present study is to develop ultraviolet (UV)-spectroscopic method using pure drug and tablet dosage form that consistently produces a drug with a minimal variation that adheres to quality criteria of purity, identity, and potency. Methods: UV-spectrophotometric method has been developed using a solvent composed of methanol:water (30:70) as a diluent to determine the dalfampridine (DFP) content in bulk and pharmaceutical dosage form at predetermined λmax of 262 nm. Results: It was proved linear in the range of 02–12 μg/ml and exhibited a good correlation coefficient (r2 = 0.9915) and excellent mean recovery (0.004136347%). This method was successfully applied to the determination of DFP content of marketed tablet Dalstep 10 mg (Sun Pharmaceutical Pvt. Ltd.,) from India; the results were in good agreement with the label claims. Conclusion: The method proved to be simple, accurate, precise, specific, robust, and less time consuming and can be applied for the determination of DFP in bulk and marketed formulation.

VALIDATED STABILITY INDICATING SPECTROSCOPIC METHOD FOR ESTIMATION OF DEGRADATION BEHAVIOUR OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLET FORMULATION

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (11) ◽  
pp. 53-59
Author(s):  
Tukaram M. Kalyankar ◽  
◽  
Shital S. Dange ◽  
Shivraj B. Hambarde ◽  
Shailesh J. Wadher ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Stress Conditions ◽  
Spectrometric Method ◽  
Degradation Behaviour ◽  
Stability Indicating ◽  
Photolytic Degradation ◽  
Tablet Dosage

A simple, accurate and precise UV spectrometric method has been developed for the simultaneous determination of valsartan and hydrochlorothiazide in tablet dosage form. Spectra of valsartan and hydrochlorothiazide in methanol and water (50:50 V/V) show λ max at 250.0 nm and 271.4 nm, respectively. Valsartan and hydrochlorothiazide are subjected to various stress conditions like acid, alkali, thermal and photolytic degradation. Beer’s law was obeyed in concentration range of 4- 24 µg mL-1 for valsartan and 0.5-3 µg mL-1 for hydrochlorothiazide at their respective wavelengths. The proposed method was successfully applied to tablet dosage form for determination of both drugs. The percentage recovery of valsartan and hydrochlorothiazide were found to be 100.19 % and 99.51 %, respectively. A novel accurate and precise stability indicating spectroscopic method has been developed for estimation of valsartan and hydrochlorothiazide.

scholarly journals A NEW VALIDATED THIRD ORDER DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND RAMIPRIL IN TABLET DOSAGE FORM

2020 ◽  
pp. 54-59
Author(s):  
ALEKHYA B. ◽  
M. SINDHUSHA ◽  
SORAJ K. RAUL ◽  
GOPAL K. PADHY
Keyword(s):  
Quantitative Analysis ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Third Order ◽  
Metoprolol Succinate ◽  
Zero Crossing ◽  
Ich Guidelines ◽  
Tablet Dosage

Objective: The objective of the present work is to develop and validate a new UV derivative spectrophotometric method for simultaneous estimation of metoprolol succinate and ramipril in methanol: water (50:50v/v). Methods: “Zero crossing technique” was chosen for quantitative determination. The zero-crossing points (ZCP’s) were found to be 209 nm where metoprolol succinate was quantified and 211 nm where ramipril was quantified. This method was then subjected to accuracy, linearity, sensitivity and reproducibility according to ICH guidelines to ensure and confirm its validity. Results: The method was found to be obeying Beer’s law in the range of 10-50 µg/ml and 5-25 µg/ml for metoprolol succinate and ramipril, respectively. The % recoveries were observed between the range of 99.2-100.2 for metoprolol succinate and 99.57-99.86 for ramipril. The intra-day and inter-day results showed reproducibility. Conclusion: It can be concluded that the developed third-order UV derivative spectroscopic method for the simultaneous determination of metoprolol succinate and ramiprilcan be recommended for routine quantitative analysis.

scholarly journals Development and validation of RP-HPLC method for determination of Atorvastatin calcium and Nicotinic acid in combined tablet dosage form

2016 ◽  
Vol 20 ◽  
pp. S328-S333 ◽  
Author(s):  
Jaiprakash N. Sangshetti ◽  
Mohammed Aqeel ◽  
Zahid Zaheer ◽  
Rana Z. Ahmed ◽  
M.H.G. Dehghan ◽  
...  
Keyword(s):  
Nicotinic Acid ◽  
Dosage Form ◽  
Hplc Method ◽  
Atorvastatin Calcium ◽  
Rp Hplc ◽  
Development And Validation ◽  
Tablet Dosage

scholarly journals Development and Validation of UV Spectroscopic Method for Estimation of Valsartan In Tablet Dosage Form

2019 ◽  
Vol 9 (6) ◽  
pp. 233-241
Author(s):  
Swapnil R. Patil ◽  
Anagha P. Patil ◽  
Prajakta D. Chaudhari ◽  
Kalpesh V. Sonar
Keyword(s):  
Dosage Form ◽  
Spectroscopic Method ◽  
Development And Validation ◽  
Tablet Dosage

scholarly journals Simultaneous UV Spectrophotometric Estimation of Amlodipine and Chlorthalidone in Bulk and Combined Tablet Dosage Form

2019 ◽  
pp. 468-472
Author(s):  
Sunil More ◽  
Ashpak Tamboli ◽  
Snehal Patil ◽  
Amol Vhanmane
Keyword(s):  
Spectrophotometric Method ◽  
Mobile Phase ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Ich Guidelines ◽  
Combined Estimation ◽  
Tablet Dosage ◽  
Different Levels

There is not a single analytical methods appeared in the literature for combined estimation of Amlodipine and Chlorthalidone in tablets dosage form. Attempts were made to develop a simple, precise and accurate Simultaneous UV spectroscopic method of Amlodipine and Chlorthalidone in bulk and Amlodac CH tablet dosage form by using simultaneous equation method. UV spectrophotometric method was developed and validated as per ICH guidelines using methanol as mobile phase. Amlodipine and Chlorthalidone individually follows the Beer-Lamberts law over concentration range 2.5-12.5μg/ml and 6.25-31.5μg/ml, Regression of coefficient was found to be r2=0.999 and r2=0.999 respectively. The percentage recovery was found in the range of 98% to 102% at three different levels. The proposed method was successfully applied for the determination of Amlodipine and Chlorthalidone in tablets dosage form as per ICH guidelines the result of the analysis were validated statistically and were found to be satisfactory.

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