Avaliação in vitro e in vivo da atividade de frações e compostos isolados de Phyllanthus amarus contra o Schistosoma mansoni linhagem BH

Soluble egg antigens of the parasitic helminth Schistosoma mansoni (S. mansoni egg antigen [SEA]) induce strong Th2 responses both in vitro and in vivo. However, the specific molecules that prime the development of Th2 responses have not been identified. We report that omega-1, a glycoprotein which is secreted from S. mansoni eggs and present in SEA, is capable of conditioning human monocyte-derived dendritic cells in vitro to drive T helper 2 (Th2) polarization with similar characteristics as whole SEA. Furthermore, using IL-4 dual reporter mice, we show that both natural and recombinant omega-1 alone are sufficient to generate Th2 responses in vivo, even in the absence of IL-4R signaling. Finally, omega-1–depleted SEA displays an impaired capacity for Th2 priming in vitro, but not in vivo, suggesting the existence of additional factors within SEA that can compensate for the omega-1–mediated effects. Collectively, we identify omega-1, a single component of SEA, as a potent inducer of Th2 responses.

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In vitro and in vivo anthelmintic activity of (−)-6,6′-dinitrohinokinin against schistosomula and juvenile and adult worms of Schistosoma mansoni

Acta Tropica ◽

10.1016/j.actatropica.2015.06.005 ◽

2015 ◽

Vol 149 ◽

pp. 195-201 ◽

Cited By ~ 19

Author(s):

Ana C. Pereira ◽

Márcio L.A. e Silva ◽

Julia Medeiros Souza ◽

Rosangela S. de Laurentiz ◽

Vanderlei Rodrigues ◽

...

Keyword(s):

Schistosoma Mansoni ◽

Anthelmintic Activity

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Elucidation of the in vitro and in vivo activities of bridged 1,2,4-trioxolanes, bridged 1,2,4,5-tetraoxanes, tricyclic monoperoxides, silyl peroxides, and hydroxylamine derivatives against Schistosoma mansoni

Bioorganic & Medicinal Chemistry ◽

10.1016/j.bmc.2015.02.010 ◽

2015 ◽

Vol 23 (16) ◽

pp. 5175-5181 ◽

Cited By ~ 24

Author(s):

Noemi Cowan ◽

Ivan A. Yaremenko ◽

Igor B. Krylov ◽

Alexander O. Terent’ev ◽

Jennifer Keiser

Keyword(s):

Schistosoma Mansoni ◽

Hydroxylamine Derivatives

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Differences between in vitro and in vivo obtained schistosomules

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651990000200003 ◽

1990 ◽

Vol 32 (2) ◽

pp. 84-85 ◽

Cited By ~ 7

Author(s):

Alan L. Melo ◽

Conceição R. S. Machado ◽

Leógenes H. Pereira

Keyword(s):

Cell Adhesion ◽

Schistosoma Mansoni ◽

Peritoneal Cavity ◽

Adherent Cells

The injection of cercariae of Schistosoma mansoni into the peritoneal cavity of naive mice induces cell adhesion to these larvae, and this adherence sharply decreases when the infecting larva changes to schistosomule. This procedure was used to detect differences between schistosomules obtained in vivo and in vitro. Reinoculation of schistosomules obtained in vivo into the peritoneal cavity of mice did not trigger cell adhesion. In contrast, adherent cells were found in 4 and 24-hour-in vitro schistosomules. Our data on schistosomules obtained in vitro indicate that more than 24 hours are needed for complete remotion of molecules involved in the phenomenon of cell adhesion.

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