scholarly journals Invitro germination of Bael (Aegle marmelos (L.) Corr.) seeds for clonal propagation

2020 ◽  
pp. 449-453
Author(s):  
INDHUMATHI K
Keyword(s):  
Medicinal Plants ◽  
Traditional Medicine ◽  
Seed Coat ◽  
Clonal Propagation ◽  
Multiple Shoots ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Aegle Marmelos ◽  
Medicinal Properties

Aegle marmelos, commonly known as Bael, is one of the most important medicinal plants of Indian tradition. All parts of the tree viz. leaf, fruit, bark and root have medicinal properties and have been used in many traditional medicine systems. The present study trial has been taken up at Horticultural College & Research Institute, Coimbatore to establish a protocol for in vitro germination of seeds to use it further for clonal propagation. The investigation revealed that the seeds without seed coat gave more number of multiple shoots (5.57) where as the seeds with seed coat gave single seedling. The days taken for germination was lesser in seeds without seed coat (14.76 days) than the the seeds with seed coat (20.59 days). The longest seedling was observed in the seeds with seed coat (2.09 cm) compared to that of seeds without seed coat (1.22 cm). the days taken for budbreak, number of shoots were higher in the MS medium supplemented with BAP 1.5 mgl-1. Comparison between the explants from in vitro and field grown seedlings showed that the time taken for culture response was much earlier in the in vitro derived seedlings. All the explants showed better response in the basal MS medium supplemented with BAP 1.5 mgl-1

scholarly journals In vitro clonal multiplication of Aegle marmelos (L.) Corr. through cotylodonary node culture

2013 ◽  
Vol 48 (1) ◽  
pp. 13-18 ◽  
Author(s):  
S Akter ◽  
TA Banu ◽  
MA Habib ◽  
S Afrin ◽  
A Khatun ◽  
...  
Keyword(s):  
Multiple Shoots ◽  
Poor Response ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Aegle Marmelos ◽  
Clonal Multiplication ◽  
Cotyledonary Nodes ◽  
Half Strength

Clonal multiplication of Aegle marmelos was achieved through in vitro culture. Cotyledonary nodes from one month old in-vitro grown seedlings of Aegle marmelos were cultured on MS medium supplemented with BAP, Kn, and IBA either alone or in combination. The highest regenerative response was observed on medium containing 2.5 mg/L BAP and 0.5 mg/L Kn from node and shoot tip within 8-10 days. When regenerated shoots were subcultured on MS medium supplemented with 0.2 mg/L BAP and 0.02 mg/L Kn, maximum number of multiple shoots were observed after third phase of subculture. Poor response was found using MS medium supplemented with Kn only. In vitro induced shoots were transferred into root induction medium consisting of half-strength MS supplemented with auxins, IAA, IBA or NAA. Rooting was best in medium supplemented with 1.0 mg/L IBA. Rooted plantlets were acclimatized and transferred to the soil with 96% survival rate. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15408 Bangladesh J. Sci. Ind. Res. 48(1), 13-18, 2013

scholarly journals In vitro Rapid Clonal Propagation of Rauvolfia serpentina (Linn.) Benth

1970 ◽  
Vol 42 (1) ◽  
pp. 37-44 ◽  
Author(s):  
R Baksha ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun ◽  
John Liton Munshi
Keyword(s):  
Large Scale ◽  
Clonal Propagation ◽  
Multiple Shoots ◽  
Direct Regeneration ◽  
Ms Medium ◽  
Rauvolfia Serpentina ◽  
Half Strength ◽  
In Vitro Clonal Propagation ◽  
Shoot Tip Explants

A protocol was developed for in vitro clonal propagation of Rauvolfia serpentina through direct regeneration from shoot tip explants. Multiple shoots (eight shoots per explant) induction were obtained on MS medium supplemented with 4.0 mg/l BAP and 0.5 mg/l NAA within 10-15 days. The elongated shoots rooted well in half strength MS medium with 0.5 mg/l NAA. The in vitro raised plantlets were acclimatized in glass house and successfully transplanted to field condition with 80 % survival. The results indicated that large scale commercial micropropagation of Rauvolfia serpentina is technically feasible. Bangladesh J. Sci. Ind. Res. 42(1), 37-44, 2007

scholarly journals Clonal propagation of Carob (Ceratonia siliqua L., Fabaceae)

1970 ◽  
Vol 39 (1) ◽  
pp. 15-19 ◽  
Author(s):  
L Hakim ◽  
MR Islam ◽  
ANK Mamun ◽  
G Ahmed ◽  
R Khan
Keyword(s):  
Clonal Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Root Induction ◽  
Ms Medium ◽  
Ceratonia Siliqua ◽  
Moderate Amount ◽  
Half Strength

Mature seeds of carob tree (Ceratonia siliqua L.) were germinated on hormone free MS medium. Efforts were made to develop multiple shoots by using axillary buds of in vitro grown seedlings on MS medium fortified with different concentrations of BA singly and BA in combination with IAA or GA3. Axillary buds produced single shoot with a moderate amount of callus at the base of the explant after culturing on MS medium with BA alone. Multiple shoots were regenerated when explants when cultured on MS medium fortified with BA + IAA or BA + GA3. MS medium supplemented with 1.5 mg/l BA + 0.5 mg/l GA3 was found more effective in multiple shoot regeneration than all other combinations. Regenerated multiple shoots were excised and cultured on half strength of MS medium containing different concentrations of IBA for root induction. Best root development was obtained in half strength MS medium containing 0.5 mg/l IBA. About 70% of the regenerated plantlets survived in natural conditions. Key words: Carob; Ceratonia siliqua; Fabaceae; Clonal propagation DOI: 10.3329/bjb.v39i1.5520Bangladesh J. Bot. 39(1): 15-19, 2010 (June)

scholarly journals An Improved Micropropagation Protocol byEx VitroRooting ofPassiflora edulisSims. f.flavicarpaDeg. through Nodal Segment Culture

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari ◽  
C. P. Ravindran
Keyword(s):  
Survival Rate ◽  
Clonal Propagation ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Nodal Segment ◽  
Ms Medium ◽  
Ex Vitro ◽  
Passiflora Edulis ◽  
Half Strength

A procedure for rapid clonal propagation ofPassiflora edulisSims. f.flavicarpaDeg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−16-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21±1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1of each BAP and Kinetin (Kin) was successful for the multiplication of the shootsin vitrowith maximum numbers of shoots (25.73±0.06) within four weeks of incubation. Shoots were rooted best (7.13±0.56roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1indole-3 butyric acid (IBA). Allin vitroregenerated shoots were rooted byex vitromethod, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

2011 ◽  
Vol 39 (1) ◽  
pp. 84 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Suttinee JINGJIT ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Multiple Shoots ◽  
Shoot Formation ◽  
In Vitro Flowering ◽  
Nodal Explants ◽  
Callus Growth ◽  
Ms Medium ◽  
Old Field ◽  
Gypsophila Paniculata ◽  
Initial Culture

A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

scholarly journals In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes

2015 ◽  
Vol 41 ◽  
pp. 71-77
Author(s):  
S. Parvin ◽  
M. Kausar ◽  
M. Enamul Haque ◽  
M. Khalekuzzaman ◽  
B. Sikdar ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Ms Medium ◽  
Cotyledonary Nodes ◽  
Cucumis Melo L

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from invitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growthregulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.Effective result was found on MS medium supplemented with 2.0 mg/l BAP, inwhich 90% and 70% cultures induced multiple shoots from nodal segments andshoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodeswere found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS mediumsupplemented with different concentrations of IBA and NAA for successful rootinduction and the effective result (up to 70%) was found in medium with 1 mg/lIBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, whereas 70% plantlets survived

scholarly journals Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (Aegle marmelos L.)

1970 ◽  
Vol 44 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Ranjoy Das ◽  
M Faruk Hasan ◽  
Harunar Rashid ◽  
Motiur Rahman
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Nodal Explant ◽  
Aegle Marmelos ◽  
Half Strength ◽  
Subsequent Regeneration

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival. Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos. DOI: 10.3329/bjsir.v44i4.4590 Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

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