scholarly journals Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus

2021 ◽  
Vol 24 (11) ◽  
pp. 370-374
Author(s):  
Ju-Won Kim ◽  
Ja Young Cho ◽  
Won-Kyong Chun ◽  
Dong-Gyun Kim ◽  
Bo-Hye Nam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Paralichthys Olivaceus ◽  
Embryonic Cell ◽  
Olive Flounder ◽  
Embryonic Cell Line ◽  
Olive Flounder Paralichthys Olivaceus

scholarly journals Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus

2021 ◽  
Vol 21 (04) ◽  
pp. 159-167
Author(s):  
Miaomiao Nie ◽  
Zhihao Wu ◽  
Feng You
Keyword(s):  
Cell Line ◽  
Paralichthys Olivaceus ◽  
Chromosome Analysis ◽  
Embryonic Cell ◽  
Olive Flounder ◽  
Coi Gene ◽  
Exponential Growth Phase ◽  
Embryonic Cell Line ◽  
C Subunit ◽  
Olive Flounder Paralichthys Olivaceus

A new embryonic cell line that consisted predominantly of fibroblast cells has been established from embryos at Kupffer’s vesicle (KV) stage of the olive flounder Paralichthys olivaceus, designated as the PoEKC. PoEKC cells have been subcultured for 61 passages over a period of 18 months. The cells were cultured in DMEM/F-12 medium supplemented with antibiotics, FBS, and growth factors at temperature of 25°C. The growth curve of the PoEKC cells comprised an exponential growth phase and a long plateau phase. Chromosome analysis revealed that the cells possessed the normal flounder diploid karyotype of 2n = 48t. The origin of the cell line was confirmed by testing the partial sequences of cytochrome oxidase c subunit I (COI) gene of mtDNA. The pluripotency markers, genes OCT4-1, SOX2, KLF4, and NANOG showed positive signals in the PoEKC cells, and the cells also presented high ALP activity. According to the above results, the PoEKC cells might be pluripotency, though the pluripotency needs further confirmation. The cells were also successfully transfected with GFP reporter gene suggesting that it could be utilized for gene function study in the flounder.

scholarly journals Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Gyu-Hwi Nam ◽  
Anshuman Mishra ◽  
Jeong-An Gim ◽  
Hee-Eun Lee ◽  
Ara Jo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Paralichthys Olivaceus ◽  
Gene Expression Profiles ◽  
Olive Flounder ◽  
Olive Flounder Paralichthys Olivaceus

Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus

2015 ◽  
Vol 34 (5) ◽  
pp. 1054-1063 ◽  
Author(s):  
Limin Peng ◽  
Yuan Zheng ◽  
Feng You ◽  
Zhihao Wu ◽  
Yuxia Zou ◽  
...  
Keyword(s):  
Cell Line ◽  
Sertoli Cell ◽  
Paralichthys Olivaceus ◽  
Olive Flounder ◽  
Olive Flounder Paralichthys Olivaceus ◽  
Sertoli Cell Line

Genomic and gene expression responses to genotoxic stress in PAC2 zebrafish embryonic cell line

2015 ◽  
Vol 35 (11) ◽  
pp. 1381-1389 ◽  
Author(s):  
Maja Šrut ◽  
Jean-Paul Bourdineaud ◽  
Anamaria Štambuk ◽  
Göran I. V. Klobučar
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Genotoxic Stress ◽  
Embryonic Cell ◽  
Embryonic Cell Line

scholarly journals Induction and Inhibition of CYP1A Gene Expression and Steroidogenesis in Olive Flounder Paralichthys olivaceus Exposed to Tributyltin and Benzo[a]pyrene

2006 ◽  
Vol 9 (2) ◽  
pp. 64-69
Author(s):  
Jee-Hyun Jung ◽  
Un-Hyuk Yim ◽  
Joong-Kyun Jeon ◽  
Ji-Seon Lee ◽  
Dae-Jung Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Paralichthys Olivaceus ◽  
Olive Flounder ◽  
Olive Flounder Paralichthys Olivaceus
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