scholarly journals Direct Tissue Blot Immunoassay (DTBIA) for Detection of Citrus Tristeza Virus (CTV)

1993 ◽  
Vol 12 (12) ◽  
Author(s):  
S. M. Garnsey ◽  
T. A. Permar ◽  
M. Cambra ◽  
C. T. Henderson
Keyword(s):  
Citrus Tristeza Virus ◽  
Tissue Blot Immunoassay ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Prereaction of Citrus tristeza virus (CTV) Specific Antibodies and Labeled Secondary Antibodies Increases Speed of Direct Tissue Blot Immunoassay for CTV

Plant Disease ◽  
2006 ◽  
Vol 90 (5) ◽  
pp. 675-679 ◽  
Author(s):  
Youjian Lin ◽  
Phyllis A. Rundell ◽  
Lianhui Xie ◽  
Charles A. Powell
Keyword(s):  
Citrus Tristeza Virus ◽  
Plant Viruses ◽  
Specific Antibodies ◽  
Rabbit Igg ◽  
Tissue Blot Immunoassay ◽  
Polymerase Chain ◽  
Bacteria And Fungi ◽  
Secondary Antibodies ◽  
Tristeza Virus ◽  
Citrus Tristeza

An improved direct tissue blot immunoassay (DTBIA) procedure for detection of Citrus tristeza virus (CTV) within 1 h is described. Prints of fresh young stems of citrus plants that were infected or not infected with CTV were made by gently and evenly pressing the fresh-cut surface of the stems onto a nitrocellulose membrane. The tissue blots were air-dried for 5 min, incubated with prereaction solutions of CTV-specific antibodies and labeled secondary antibodies, goat anti-mouse Ig (H+L)-alkaline phosphatase conjugate or goat anti-rabbit IgG alkaline phos-phatase conjugate, for up to 20 min, rinsed with PBST buffer for 5 min, and immersed into an NBT-BCIP substrate solution for 15 to 20 min. Then the blots were rinsed in water for a few seconds to stop the reactions, and the results were observed and recorded under a light microscope. All samples from greenhouse plants that were infected with CTV decline inducing isolate T-36 were positive to CTV-specific polyclonal antibody 1212 (PCA 1212) and monoclonal antibodies 17G11 (MAb 17G11) and MCA13 (MAb MCA13), whereas samples from greenhouse plants infected with non-decline-inducing isolate T-30 were positive to PCA 1212 and MAb 17G11, but not to MAb MCA13. The noninfected greenhouse plants were negative to all of the antibodies. The improved DTBIA was at least as reliable as other immunological procedures and almost as reliable as polymerase chain reaction for detecting CTV in field trees. The improved DTBIA enables the detection of CTV within 1 h by having a prereaction of CTV-specific antibodies and labeled secondary antibodies in solutions before they are applied to the tissue blots. This DTBIA procedure may be useful in detecting other plant viruses and other pathogens such as bacteria and fungi.

First report of citrus tristeza virus in commercial citrus orchards in Tunisia

2021 ◽  
Author(s):  
Asma Najar ◽  
Imen Hamdi ◽  
Souad Mahmoud ◽  
Lassaad Medhioub ◽  
Imed Jaouadi ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Citrus Orchards ◽  
Tristeza Virus ◽  
Citrus Tristeza

A model for the spread and control of citrus tristeza virus disease

1989 ◽  
Vol 16 (3) ◽  
pp. 315-320
Author(s):  
Ruth Marcus ◽  
Hovav Talpaz ◽  
Moshe Bar-Joseph
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
And Control ◽  
Tristeza Virus ◽  
Citrus Tristeza

Sscp analysis of variations in haplotypes of citrus tristeza virus isolated from yuzu (Citrus junos) in geographically separate regions of Korea

2006 ◽  
Vol 49 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Dae Hyun Kim ◽  
Hye Kyung Shim ◽  
Jae Wook Hyeon ◽  
Hyeog Mo Kwon ◽  
Kwang Sik Kim ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Sscp Analysis ◽  
Citrus Junos ◽  
Tristeza Virus ◽  
Citrus Tristeza

Production of Antibodies to Citrus Tristeza Virus in Transgenic Citrus

1995 ◽  
Author(s):  
Richard Lee ◽  
Moshe Bar-Joseph ◽  
K.S. Derrick ◽  
Aliza Vardi ◽  
Roland Brlansky ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
Biological Activities ◽  
Cp Gene ◽  
Single Domain Antibodies ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) is the most important virus disease of citrus in the world. CTV causes death of trees on sour orange rootstock and/or stem pitting of scions regardless of rootstock which results in trees of low vigor, reduced yield with reduction in size and quality of fruit. The purpose of this project was to produce monoclonal antibodies (MABs) to CTV coat protein (CP), develop single domain antibodies (dAbs) or Fab fragments which neutralize the infection by binding to the virus, and to produce transformed plants which express the dAbs. The objectives of this research have been met and putative transgenic tobacco and citrus plants have been developed. These putative transgenic plants are presently undergoing evaluation to determine the level of dAbs expression and to determine their resistance to CTV. Additionally, the CTV genome has been sequenced and the CP gene of several biologically characterized CTV strains molecular characterized. This has indicated a correlation between CP sequence homology and biological activity, and the finding of DI RNAs associated with some CTV strains. Several MABs have been produced which enable broad spectrum identification of CTV strains while other MABs enable differentiation between mild and severe strains. The use of selected MAbs and determination of the CP gene sequence has enabled predictions of biological activities of unknown CTV isolates. The epitopes of two MABs, one reacting selectively with severe CTV strains and the other reacting with all strains, have been characterized at the molecular level.

scholarly journals Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program

2013 ◽  
Vol 70 (2) ◽  
pp. 116-124 ◽  
Author(s):  
Carlos Alexandre Zanutto ◽  
Maria Júlia Corazza ◽  
William Mário de Carvalho Nunes ◽  
Gerd Walter Müller
Keyword(s):  
Citrus Tristeza Virus ◽  
Protective Capacity ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Eliminação de vírus do complexo sorose dos citros por microenxertia associada a termoterapia

2002 ◽  
Vol 27 (3) ◽  
pp. 306-308 ◽  
Author(s):  
SÉRGIO A. CARVALHO ◽  
FRANCISCA A. SANTOS ◽  
MARCOS A. MACHADO
Keyword(s):  
Citrus Tristeza Virus ◽  
Citrus Exocortis Viroid ◽  
Tristeza Virus ◽  
Citrus Tristeza

A microenxertia de ápices caulinares tem sido utilizada com 100% de sucesso na eliminação do vírus da tristeza (Citrus tristeza virus) e dos viróides da exocorte (Citrus exocortis viroid - CEVd) e cachexia-xiloporose de materiais do Banco Ativo de Germoplasma de Citros do Centro de Citricultura Sylvio Moreira CCSM-IAC. Para o complexo da sorose, entretanto, esta técnica tem apresentado somente 60% de eficiência, indicando a necessidade de sua associação com termoterapia para garantir a eliminação viral. Para tanto, mudas originadas de borbulhas infetadas com sorose foram mantidas em câmara climática com 16 h de luz a 38 ºC e 8 h no escuro a 32 ºC e utilizadas para a obtenção dos ápices caulinares empregados na microenxertia. Após o pegamento, o conjunto micro porta-enxerto e brotação foi sobre-enxertado em limoeiro (Citrus limonia) 'Cravo' com sete meses de idade e mantido em condições de casa de vegetação. Clones de laranjeiras doces (Citrus sinsensis) 'Lima', 'Rubi', 'Piralima', 'Salustiana', 'João Nunes', 'Rosa' e 'Pêra Caire' tratados desta maneira, comprovaram eficiência de 100% de eliminação do complexo sorose, conforme indexação realizada empregando-se laranjeira 'Do Céu' como planta indicadora.

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