Molecular cloning, sequence characterization, and tissue expression analysis
of three water buffalo (&lt;i&gt;Bubalus bubalis&lt;/i&gt;) genes – &lt;i&gt;ST6GAL1&lt;/i&gt;,
&lt;i&gt;ST8SIA4&lt;/i&gt;, and &lt;i&gt;SLC35C1&lt;/i&gt;

Abstract. Recent studies have shown that ST6 beta-galactosamide alpha-2,6-sialyltransferase 1 (ST6GAL1), ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4), and solute carrier family 35, member C1 (SLC35C1) play essential roles in the metabolism of milk glycoconjugates in mammals. However, studies on their coding genes in water buffalo have not been reported. In the present study, cloning and sequencing showed that the coding sequences (CDSs) of buffalo ST6GAL1, ST8SIA4, and SLC35C1 were 1218, 1080, and 1095 bp in length, which encoded a precursor protein composed of 405, 359, and 364 amino acids, respectively. The deduced sequences of these three proteins in turn showed 97.6–98.5, 98.6–99.7, and 97.8–99.2 % similarities with other bovine species. Both buffalo ST6GAL1 and ST8SIA4 were predicted to be a member of glycosyltransferase family 29 and were all hydrophilicity proteins functioning in the Golgi apparatus. Buffalo SLC35C1 was a hydrophobic membrane protein located in the Golgi membrane, containing a TPT domain that is found in a number of sugar phosphate transporters. In addition, semi-quantitative RT-PCR analysis in 13 lactating buffalo tissues revealed that the ST6GAL1 and ST8SIA4 were expressed in 9 tissues, while SLC35C1 was expressed in 11 tissues. The expression levels of these three genes in the mammary gland were significantly higher in lactating than in non-lactating stage. Collectively, our data indicate that ST6GAL1, ST8SIA4, and SLC35C1 are potentially involved in the process of buffalo lactation.

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Molecular cloning, sequence characterization, and gene expression profile of a novel water buffalo (Bubalus bubalis) gene: Na+, K+-ATPase β2-subunit (ATP1B2)

Archives Animal Breeding ◽

10.7482/0003-9438-57-015 ◽

2014 ◽

Vol 57 (1) ◽

pp. 1-12

Author(s):

Shen Song ◽

Jinlong Huo ◽

Feng Yuan ◽

Yina Ou-Yang ◽

DaLin Li ◽

...

Keyword(s):

Water Buffalo ◽

High Reliability ◽

Expression Patterns ◽

Tissue Expression ◽

Bubalus Bubalis ◽

Homologous Sequence ◽

Amino Acid Residues ◽

Phylogenetic Tree Analysis ◽

Hydrophobic Region ◽

Sequence Characterization

Abstract. The Na+, K+-ATPase is a transmembrane carrier protein which plays an important role in Na-K transport and energy metabolism. The aim of this study was to obtain the full-length coding sequence (CDS) of water buffalo Na+, K+-ATPase β2-subunit (ATP1B2) using RT-PCR and to investigate the characterizations of its sequence and tissue expression patterns. Sequence analysis revealed that the CDS of water buffalo ATP1B2 encodes an enzyme of 290 amino acid residues with a deduced molecular weight of 33.39 KDa and a PI of 8.37. Water buffalo ATP1B2 was presumed to have a signal peptide, a strong hydrophobic region and to exert its function in the plasma membrane with high reliability. In addition, water buffalo ATP1B2 has a conserved Na+, K+-ATPase β domain which belongs to Na+, K+-ATPase superfamily. The sequence of water buffalo ATP1B2 gene shares 97.6, 97.4, 92.2, 93.2, 89.9, 93.2, 89.5 and 62.9 % identify with its homologous sequence of cattle, sheep, dog, human, mouse, baboon, rat and African clawed frog, respectively. Phylogenetic tree analysis based on the CDS of ATP1B2 gene showed that water buffalo has a closer genetic relationship with cattle than with other species. The ATP1B2 gene was widely expressed in the tissues examined, being high in the pituitary gland and brain, moderate in the muscle, spleen, liver, mammary gland, kidney and rumen, weak in the heart, small intestines and skin, and not expressed in the lung and adipose tissue. This study will establish a foundation for further insights into this novel water buffalo gene.

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Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA

International Journal of Immunogenetics ◽

10.1111/j.1744-313x.2011.01075.x ◽

2011 ◽

Vol 39 (3) ◽

pp. 203-206

Author(s):

S. Thennarasu ◽

M. Harishankar ◽

G. Dhinakar Raj

Keyword(s):

Water Buffalo ◽

Bubalus Bubalis ◽

Cloning And Sequencing ◽

Indian Water ◽

Interleukin 3

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Differences in plasma Anti Müllerian hormone levels and reproductive parameters between two bovine species: Bos indicus and Bubalus bubalis

Veterinarska stanica ◽

10.46419/vs.51.5.3 ◽

2020 ◽

Vol 51 (5) ◽

pp. 497-506

Author(s):

Jesús A. Berdugo ◽

Ariel M. Tarazona ◽

José de Julián Echeverri ◽

Walter D. Cardona-Maya ◽

Albeiro Lopez-Herrera

Keyword(s):

Clinical Data ◽

Ovarian Reserve ◽

Water Buffalo ◽

Bos Indicus ◽

Bubalus Bubalis ◽

Zebu Cattle ◽

Reproductive Parameters ◽

Water Buffaloes ◽

Days Open ◽

Bovine Species

The aim of this study was to evaluate the possible relationships between reproductive parameters and Anti Mullerian Hormone (AMH) levels in two closely related bovine species. AMH levels and the reproductive parameters of 50 water buffaloes (Bubalus bubalis) and 50 Zebu cattle (Bos indicus) of the same age and raised in similar conditions were evaluated. Clinical data were obtained from the farm in specialized designed format, and AMH was measured by ELISA. The results show that Zebu cattle had higher AMH levels than water buffaloes (P<0.0001), while water buffaloes exhibited better reproductive parameters: days open to pregnancy (P<0.0001), parity (P<0.0005), age at first calving (P<0.0001), and intercalving period (P<0.0001). Although both species have different reproductive parameters, no correlations were found between AMH and the reproductive parameters evaluated in either species. Finally, pregnant Zebu cattle and water buffalo showed a tendency to have lower AMH levels than non-pregnant counterparts. The results are paradoxical as the species with lower AMH levels exhibited better reproductive parameters, suggesting that the reproductive parameters within the herd do not depend solely on the ovarian reserve of females.

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