Using X-ray radiography to image rapid water infiltration into soil

Author(s):  
John Koestel ◽  
Lorenzo Garbari ◽  
Daniel Iseskog

<p>While the basic processes of water infiltration into soil are well understood, their details are difficult to quantify due to the opaque nature of soil. In this study, we investigated the potential and limitations of X-ray radiography to measure the water front progression in a narrow sample (15 × 15 × 1 cm) filled with dry soil under simulated rainfall of high intensity (53 mm/h). The temporal resolution of the acquired infiltration movies was 133 milliseconds. We evaluated three different kinds of soil samples. i) Bare samples filled with a 1:1 mixture of a sandy loam and peat. ii) The same soil-peat mixture, but cropped with <em>Trifolium incarnatum</em>, <em>Trifolium repens</em>, <em>Lathyrus odoratus</em> and <em>Lupinus regalis</em>, all of them plants that have been reported to induce water repellency; prior to the experiments, the plants were harvested and only the roots remained in place. iii) Sandy loam soil that had been incubated for four weeks in an outside garden plot. Due to time limitations of the project, the incubation period was in early spring, which meant that plant growth in the samples was negligible. All three sample types were replicated five times, resulting in 15 individual samples. We carried out the infiltration experiments in four-fold replications, from which it follows that we collected 60 individual infiltration movies. After each infiltration round, the samples were placed in a drying room to reset them to a similar initial moisture content. The experiments aimed at testing i) whether the infiltration patterns of the four consecutive infiltration runs conducted on each sample remained identical and ii) to document differences in infiltration patterns between bare, cropped and incubated samples. We found that increasing X-ray scattering with increasing soil water contents made it challenging to evaluate the image data quantitatively. Advantages of our setup are that X-ray captures the complete water content at a specific depth and that sample boxes with irregularly shaped walls can be used to prevent preferential flow along the walls. Preliminary analyses of the data showed that the infiltration fronts in the bare and the incubated samples were less uniform than the ones for the cropped samples. In contrast, the likelihood of observing the same infiltration pattern in all four consecutive infiltration runs was larger for the bare and the incubated samples. The latter fact may have been caused by the interaction with root exudates in the cropped samples that may have been redistributed or mineralized during the wetting-drying cycles. We conclude that the here presented setup has large potential to investigate unstable infiltration phenomena into soil after an image correction approach has been developed that removes X-ray scattering artifacts. Alternatively, scattering may be suppressed by using a collimator during image acquisition.</p>

Author(s):  
Eva-Maria Mandelkow ◽  
Eckhard Mandelkow ◽  
Joan Bordas

When a solution of microtubule protein is changed from non-polymerising to polymerising conditions (e.g. by temperature jump or mixing with GTP) there is a series of structural transitions preceding microtubule growth. These have been detected by time-resolved X-ray scattering using synchrotron radiation, and they may be classified into pre-nucleation and nucleation events. X-ray patterns are good indicators for the average behavior of the particles in solution, but they are difficult to interpret unless additional information on their structure is available. We therefore studied the assembly process by electron microscopy under conditions approaching those of the X-ray experiment. There are two difficulties in the EM approach: One is that the particles important for assembly are usually small and not very regular and therefore tend to be overlooked. Secondly EM specimens require low concentrations which favor disassembly of the particles one wants to observe since there is a dynamic equilibrium between polymers and subunits.


Author(s):  
Eva-Maria Mandelkow ◽  
Ron Milligan

Microtubules form part of the cytoskeleton of eukaryotic cells. They are hollow libers of about 25 nm diameter made up of 13 protofilaments, each of which consists of a chain of heterodimers of α-and β-tubulin. Microtubules can be assembled in vitro at 37°C in the presence of GTP which is hydrolyzed during the reaction, and they are disassembled at 4°C. In contrast to most other polymers microtubules show the behavior of “dynamic instability”, i.e. they can switch between phases of growth and phases of shrinkage, even at an overall steady state [1]. In certain conditions an entire solution can be synchronized, leading to autonomous oscillations in the degree of assembly which can be observed by X-ray scattering (Fig. 1), light scattering, or electron microscopy [2-5]. In addition such solutions are capable of generating spontaneous spatial patterns [6].In an earlier study we have analyzed the structure of microtubules and their cold-induced disassembly by cryo-EM [7]. One result was that disassembly takes place by loss of protofilament fragments (tubulin oligomers) which fray apart at the microtubule ends. We also looked at microtubule oscillations by time-resolved X-ray scattering and proposed a reaction scheme [4] which involves a cyclic interconversion of tubulin, microtubules, and oligomers (Fig. 2). The present study was undertaken to answer two questions: (a) What is the nature of the oscillations as seen by time-resolved cryo-EM? (b) Do microtubules disassemble by fraying protofilament fragments during oscillations at 37°C?


1992 ◽  
Vol 2 (6) ◽  
pp. 899-913 ◽  
Author(s):  
Patrick Davidson ◽  
Elisabeth Dubois-Violette ◽  
Anne-Marie Levelut ◽  
Brigitte Pansu

1996 ◽  
Vol 6 (8) ◽  
pp. 1085-1094 ◽  
Author(s):  
A. Gibaud ◽  
J. Wang ◽  
M. Tolan ◽  
G. Vignaud ◽  
S. K. Sinha

2002 ◽  
Vol 12 (6) ◽  
pp. 385-390 ◽  
Author(s):  
J.-F. Bérar ◽  
L. Blanquart ◽  
N. Boudet ◽  
P. Breugnon ◽  
B. Caillot ◽  
...  

2007 ◽  
Vol 2007 (suppl_26) ◽  
pp. 247-252
Author(s):  
R. Kužel ◽  
L. Nichtová ◽  
Z. Matěj ◽  
D. Heřman ◽  
J. Šicha ◽  
...  

2007 ◽  
Vol 2007 (suppl_26) ◽  
pp. 247-252 ◽  
Author(s):  
R. Kužel ◽  
L. Nichtová ◽  
Z. Matěj ◽  
D. Heřman ◽  
J. Šicha ◽  
...  

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