scholarly journals Separating Local and Propagated Contributors to the Behnke-fried Microelectrode Recordings

2021 ◽  
Author(s):  
P. Jurczynski ◽  
S. Cam ◽  
B. Rossion ◽  
R. Ranta
Keyword(s):  
Microelectrode Recordings

Nitric oxide decreases pacemaker activity in lymphatic vessels of guinea pig mesentery

2001 ◽  
Vol 280 (6) ◽  
pp. H2707-H2716 ◽  
Author(s):  
Pierre-Yves von der Weid ◽  
Jun Zhao ◽  
Dirk F. Van Helden
Keyword(s):  
Nitric Oxide ◽  
Guinea Pig ◽  
Kinase Inhibitors ◽  
Lymphatic Vessels ◽  
Soluble Guanylyl Cyclase ◽  
Protein Kinase Inhibitors ◽  
Pacemaker Activity ◽  
Dependent Protein Kinase ◽  
Microelectrode Recordings ◽  
Dependent Protein

Intracellular microelectrode recordings were used to determine whether nitric oxide (NO), affects the pacemaker events that initiate vasomotion in lymphatic vessels of the guinea pig mesentery. This pacemaker activity is recorded as spontaneous transient depolarizations (STDs) and is likely to arise through synchronized Ca2+ release from intracellular stores. We show here that acetylcholine-induced endothelium-derived NO and exogenous NO released by sodium nitroprusside (SNP; 100 μM) and DEA-NONOate (500 μM) reduced the frequency and amplitude of STDs. This inhibition of STD frequency and amplitude was independent of the NO-induced hyperpolarization of the smooth muscle. The SNP-induced inhibition of STD frequency and amplitude was abolished during superfusion with the soluble guanylyl cyclase inhibitor ODQ (10 μM) and was diminished in the presence of cGMP and cAMP-dependent protein kinase inhibitors. The data are consistent with the hypothesis that NO inhibits vasomotion primarily by production of cGMP and activation of both cGMP- and cAMP-dependent protein kinases, which reduce the size and frequency of STDs, probably by acting on the underlying synchronized Ca2+ release from intracellular stores.

Using a comb filter to describe time-varying biological rhythmicities

1980 ◽  
Vol 48 (3) ◽  
pp. 557-561 ◽  
Author(s):  
P. J. Brusil ◽  
T. B. Waggener ◽  
R. E. Kronauer
Keyword(s):  
High Pressure ◽  
Temperature Control ◽  
Research Unit ◽  
Internal Controls ◽  
Comb Filter ◽  
Time Varying ◽  
High Pressure Chamber ◽  
Internal Temperature ◽  
Microelectrode Recordings ◽  
Continuous Perfusion

A modular high-pressure chamber is described. This chamber will allow stable microelectrode recordings to be made for a variety of intracellular preparations at pressure of 200-300 atmospheres absolute. Its features include internal temperature control, easy visibility, continuous perfusion, electrical penetrations, and manipulation of several internal controls while the system is at pressure. The small size and high versatility of the Wilson chamber make it a convenient and inexpensive research unit for experiments in the moderately high-pressure ranges that affect biological preparations.

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