Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method

2021 ◽  
Vol 43 (1) ◽  
pp. 80-80
Author(s):  
Cheng Zhao Cheng Zhao ◽  
Qing Song Yao Qing Song Yao ◽  
Can Wang Can Wang ◽  
Mu Tan Luo Mu Tan Luo ◽  
Chao Huang Chao Huang ◽  
...  
Keyword(s):  
Lipid Content ◽  
Oleaginous Yeast ◽  
Nile Red ◽  
Gravimetric Method ◽  
Microbial Lipid ◽  
Lignocellulosic Hydrolysates ◽  
Acid Hydrolysate ◽  
Fluorescent Method ◽  
Corncob Acid Hydrolysate ◽  
Trichosporon Dermatis

To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.

Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method

2021 ◽  
Vol 43 (1) ◽  
pp. 80-80
Author(s):  
Cheng Zhao Cheng Zhao ◽  
Qing Song Yao Qing Song Yao ◽  
Can Wang Can Wang ◽  
Mu Tan Luo Mu Tan Luo ◽  
Chao Huang Chao Huang ◽  
...  
Keyword(s):  
Lipid Content ◽  
Oleaginous Yeast ◽  
Nile Red ◽  
Gravimetric Method ◽  
Microbial Lipid ◽  
Lignocellulosic Hydrolysates ◽  
Acid Hydrolysate ◽  
Fluorescent Method ◽  
Corncob Acid Hydrolysate ◽  
Trichosporon Dermatis

To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.

Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method

2021 ◽  
Vol 43 (1) ◽  
pp. 80-80
Author(s):  
Cheng Zhao Cheng Zhao ◽  
Qing Song Yao Qing Song Yao ◽  
Can Wang Can Wang ◽  
Mu Tan Luo Mu Tan Luo ◽  
Chao Huang Chao Huang ◽  
...  
Keyword(s):  
Lipid Content ◽  
Oleaginous Yeast ◽  
Nile Red ◽  
Gravimetric Method ◽  
Microbial Lipid ◽  
Lignocellulosic Hydrolysates ◽  
Acid Hydrolysate ◽  
Fluorescent Method ◽  
Corncob Acid Hydrolysate ◽  
Trichosporon Dermatis

To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.

Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method

2019 ◽  
Vol 49 (6) ◽  
pp. 597-605 ◽  
Author(s):  
Cheng Zhao ◽  
Mu-Tan Luo ◽  
Chao Huang ◽  
Xue-Fang Chen ◽  
Lian Xiong ◽  
...  
Keyword(s):  
Lipid Content ◽  
Nile Red ◽  
Oleaginous Yeasts ◽  
Intracellular Lipid ◽  
Fluorescent Method

scholarly journals Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kukkala Kiran Kumar ◽  
Farha Deeba ◽  
Sauraj ◽  
Yuvraj Singh Negi ◽  
Naseem A. Gaur
Keyword(s):  
High Throughput Screening ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cetane Number ◽  
Nile Red ◽  
Major Fatty Acid ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Dry Cell Weight ◽  
Pre Treatment

Abstract Background To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

Bioconversion of Corncob Acid Hydrolysate into Microbial Oil by the Oleaginous Yeast Lipomyces starkeyi

2013 ◽  
Vol 172 (4) ◽  
pp. 2197-2204 ◽  
Author(s):  
Chao Huang ◽  
Xue-Fang Chen ◽  
Xiao-Yan Yang ◽  
Lian Xiong ◽  
Xiao-Qing Lin ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Microbial Oil ◽  
Lipomyces Starkeyi ◽  
Acid Hydrolysate ◽  
Corncob Acid Hydrolysate

Microbial oil production from corncob acid hydrolysate by oleaginous yeast Trichosporon coremiiforme

2013 ◽  
Vol 49 ◽  
pp. 273-278 ◽  
Author(s):  
Chao Huang ◽  
Xue-fang Chen ◽  
Lian Xiong ◽  
Xiao-yan Yang ◽  
Xin-de Chen ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Oil Production ◽  
Microbial Oil ◽  
Acid Hydrolysate ◽  
Corncob Acid Hydrolysate

scholarly journals Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification

2021 ◽  
Vol 12 ◽  
Author(s):  
Mauricio Ramírez-Castrillón ◽  
Victoria P. Jaramillo-Garcia ◽  
Helio Lopes Barros ◽  
João A. Pegas Henriques ◽  
Valter Stefani ◽  
...  
Keyword(s):  
Lipid Content ◽  
High Throughput Screening ◽  
Oleaginous Yeast ◽  
Neutral Lipids ◽  
Nile Red ◽  
Oleaginous Yeasts ◽  
Red Fluorescence ◽  
Fluorescence Measurements ◽  
Yeast Strains ◽  
Nile Red Fluorescence

High-throughput screening methodologies to estimate lipid content in oleaginous yeasts use Nile red fluorescence in a given solvent and optimized excitation/emission wavelengths. However, Nile red fluorescence stabilization has been poorly analyzed, and high variability occurs when relative fluorescence is measured immediately or a few minutes after dye addition. The aim of this work was to analyze the fluorescence of Nile red at different incubation times using a variety of solvents and oleaginous/non-oleaginous yeast strains. We showed that fluorescence stabilization occurs between 20 and 30 min, depending on the strain and solvent. Therefore, we suggest that fluorescence measurements should be followed until stabilization, where Relative Fluorescence Units should be considered after stabilization for lipid content estimation.

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