scholarly journals Isolation and Characterization of a Novel Bacterium, Bacillus subtilis HR-1019, with Insoluble Phosphates Solubilizing Activity

2013 ◽  
Vol 23 (2) ◽  
pp. 242-248 ◽  
Author(s):  
Yong-Suk Lee ◽  
Dong-Ju Park ◽  
Jae Hoon Kim ◽  
Hyeong Seok Kim ◽  
Yong-Lark Choi
2009 ◽  
Vol 107 (1) ◽  
pp. 38-41 ◽  
Author(s):  
Shusuke Takahashi ◽  
Toshiki Furuya ◽  
Yoshitaka Ishii ◽  
Kuniki Kino ◽  
Kohtaro Kirimura

2015 ◽  
Vol 53 (4) ◽  
pp. 262-271 ◽  
Author(s):  
S. Durairaju Nisshanthini ◽  
Antony K. Teresa Infanta S. ◽  
Duraisamy Senthil Raja ◽  
Karuppannan Natarajan ◽  
M. Palaniswamy ◽  
...  

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Naomi Shimokawa-Chiba ◽  
Claudia Müller ◽  
Keigo Fujiwara ◽  
Bertrand Beckert ◽  
Koreaki Ito ◽  
...  

AbstractRescue of the ribosomes from dead-end translation complexes, such as those on truncated (non-stop) mRNA, is essential for the cell. Whereas bacteria use trans-translation for ribosome rescue, some Gram-negative species possess alternative and release factor (RF)-dependent rescue factors, which enable an RF to catalyze stop-codon-independent polypeptide release. We now discover that the Gram-positive Bacillus subtilis has an evolutionarily distinct ribosome rescue factor named BrfA. Genetic analysis shows that B. subtilis requires the function of either trans-translation or BrfA for growth, even in the absence of proteotoxic stresses. Biochemical and cryo-electron microscopy (cryo-EM) characterization demonstrates that BrfA binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation. Although BrfA is distinct from E. coli ArfA, they use convergent strategies in terms of mode of action and expression regulation, indicating that many bacteria may have evolved as yet unidentified ribosome rescue systems.


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