salmonella gallinarum
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2022 ◽  
Vol 12 ◽  
Author(s):  
Syeda Zainab Ilyas ◽  
Hafsa Tariq ◽  
Abdul Basit ◽  
Hamza Tahir ◽  
Zulquernain Haider ◽  
...  

Salmonella gallinarum is a poultry restricted-pathogen causing fowl-typhoid disease in adult birds with mortality rates up-to 80% and exhibit resistance against commonly used antibiotics. In this current study, a temperate broad host range bacteriophage SGP-C was isolated against S. gallinarum from poultry digesta. It showed infection ability in all the 15 tested field strains of S. gallinarum. The SGP-C phage produced circular, turbid plaques with alternate rings. Its optimum activity was observed at pH 7.0 and 37–42°C, with a latent period of 45 min and burst size of 187 virions/bacterial cell. The SGP-C lysogens, SGPC-L5 and SGPC-L6 exhibited super-infection immunity against the same phage, an already reported feature of lysogens. A virulence index of 0.5 and 0.001 as MV50 of SGP-C suggests its moderate virulence. The genome of SGP-C found circular double stranded DNA of 42 Kbp with 50.04% GC content, which encodes 63 ORFs. The presence of repressor gene at ORF49, and absence of tRNA sequence in SGP-C genome indicates its lysogenic nature. Furthermore, from NGS analysis of lysogens we propose that SGP-C genome might exist either as an episome, or both as integrated and temporary episome in the host cell and warrants further studies. Phylogenetic analysis revealed its similarity with Salmonella temperate phages belonging to family Siphoviridae. The encoded proteins by SGP-C genome have not showed homology with any known toxin and virulence factor. Although plenty of lytic bacteriophages against this pathogen are already reported, to our knowledge SGP-C is the first lysogenic phage against S. gallinarum reported so far.


2021 ◽  
Author(s):  
L. C. F. CRUZ ◽  
S. A. SAMPAIO ◽  
T. F. COSTA ◽  
S. L. S. A. ALEXANDRINO ◽  
C. S. SOUZA ◽  
...  

O trato gastrointestinal das aves é colonizado por microrganismos benéficos e maléficos, vivendo de maneira comensal. As bactérias benéficas influenciam positivamente a integridade intestinal, funcionalidade da barreira de proteção, resposta imunológica, digestão e absorção dos nutrientes. As bactérias maléficas causadoras de enterites nas aves são: Clostridium colinum (enterite ulcerativa), Clostridium perfringens (enterite necrótica), Campylobacter jejuni e Campylobacter coli (enterite hemorrágica), Escherichia coli, Salmonella entérica (Salmonella pullorum - pulorose e Salmonella gallinarum - tifo aviário). Os protozoários causadores de enterites são: Eimeria acervulina, Eimeria maxima, Eimeria tenella (causam a coccidiose), Cryptosporidium meleagridis, Cryptosporidium baileyi e Cryptosporidium galli. Os vírus que acometem aves são: Paramoxivírus aviário tipo I (doença de Newcastle), rotavírus, astrovírus e reovírus. Porém, as lesões intestinais provocadas pelo microbioma maléfico podem ser amenizadas com o uso de prebióticos e probióticos na dieta das aves. Os prebióticos e probióticos fortalecem o sistema imunológico da ave, previne contra doenças entéricas infecciosas e melhora a digestão e absorção intestinal. Algumas bactérias que pertencem a microbiota intestinal das aves são utilizadas como probióticos, as espécies mais utilizadas pertencem ao gênero Lactobacillus e Bifidobacterium. Esta revisão analisa as informações científicas disponíveis sobre os principais microrganismos que afetam a saúde intestinal das aves e o uso de prebióticos e probióticos como aditivos preventivos na literatura vigente.


Author(s):  
A Sikandar ◽  
H Zaneb ◽  
A Nasir ◽  
A Rehman ◽  
M Kashif ◽  
...  

The effect of Bacillus subtilis on the immune responses and morphometry of the immune organs was evaluated in broilers challenged with S. gallinarum.  For this purpose, Salmonella-free birds (n = 240) were split into four groups with six replicates of ten birds each. Groups included an NC (negative control, non-infected + non-medicated), a PC-S (positive control, Salmonella-infected + non-medicated), an AT-S (Salmonella-infected + medicated with enrofloxacin), and a BS-S (Salmonella-infected + B. subtilis (2.0 × 10<sup>10</sup> cfu/g; 0.1 g/kg) group. On day 21, the thickness of the thymus cortex and medulla, germinal centre area of the spleen, bursal follicular length and bursal follicular area increased (P &lt; 0.05) in the BS-S when compared to the NC and PC-S groups. On day 35, the BS-S group exhibited a higher (P &lt; 0.05) antibody titre against the Newcastle disease virus (NDV), and cortex of the thymus was thicker (P &lt; 0.05) compared to the other groups. A decrease in the thymus medulla thickness, germinal area of the spleen and bursal follicular number were noted in the PC-S group when compared to the other treatment groups. In conclusion, the prophylactic use of B. subtilis type probiotics alleviated the stress resulting from a Salmonella gallinarum infection and improved the immune organs development and function in infected broilers.


2021 ◽  
Vol 4 (4) ◽  
pp. 4852-4860
Author(s):  
Xoana Ortiz ◽  
Ricardo Anselmo ◽  
Florencia Prosdócimo ◽  
Silvia Viora ◽  
Mauricio De Franceschi ◽  
...  

Los bacteriófagos constituyen una novedad como bactericidas. Un cóctel de bacteriófagos F9 y F15, específicos para Salmonella Enteritidis (SE) y FSG, específico para Salmonella Gallinarum, fue utilizado sobre superficies contaminadas experimentalmente con SE. Quince rectángulos de polipropileno de 330 cm2 y quince trozos de rejas metálicas de 50 cm2, fueron sumergidas en cultivo de 18 h de SE incubadas una hora a 37ºC. Las unidades formadoras de colonia (UFC)/ml de SE fueron determinadas en las dos superficies y sometidas a: Tratamiento (T) 1: 5 materiales fueron rociados con 109 unidades formadoras de placa (UFP)/ml de FSG, F9 y F15; T2: 5 materiales fueron rociadas con 109 UFP/ml de F9 y FSG; T3: 5 materiales fueron rociadas con solución fisiológica.  Los resultados determinaron que T1 disminuyó significativamente la carga bacteriana sobre metal de 4,3 log10 UFC/cm2 a 2,5 ± 0,22 log10 UFC/cm2, mientras que en superficies plásticas redujo la concentración de SE en 1,23 ± 0,26  log10 UFC/cm2 (ANOVA). Se concluye que el uso de bacteriófagos contribuiría al control de SE en producción de huevos.


2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Sravya Sreekantapuram ◽  
Christian Berens ◽  
Stefanie A. Barth ◽  
Ulrich Methner ◽  
Angela Berndt

AbstractSalmonella enterica ssp. enterica serovars Enteritidis (SE) and Gallinarum (SG) cause different diseases in chickens. However, both are able to reach the blood stream where heterophils and monocytes are potentially able to phagocytose and kill the pathogens. Using an ex vivo chicken whole blood infection model, we compared the complex interactions of the differentially host-adapted SE and SG with immune cells in blood samples of two White Leghorn chicken lines showing different laying performance (WLA: high producer; R11: low producer). In order to examine the dynamic interaction between peripheral blood leucocytes and the Salmonella serovars, we performed flow cytometric analyses and survival assays measuring (i) leucocyte numbers, (ii) pathogen association with immune cells, (iii) Salmonella viability and (iv) immune gene transcription in infected whole blood over a four-hour co-culture period. Inoculation of blood from the two chicken lines with Salmonella led primarily to an interaction of the bacteria with monocytes, followed by heterophils and thrombocytes. We found higher proportions of monocytes associated with SE than with SG. In blood samples of high producing chickens, a decrease in the numbers of both heterophils and Salmonella was observed. The Salmonella challenge induced transcription of interleukin-8 (IL-8) which was more pronounced in SG- than SE-inoculated blood of R11. In conclusion, the stronger interaction of monocytes with SE than SG and the better survivability of Salmonella in blood of low-producer chickens shows that the host–pathogen interaction and the strength of the immune defence depend on both the Salmonella serovar and the chicken line.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Degpal Singh ◽  
Mithilesh Singh ◽  
Vishal Chander ◽  
Gaurav Kumar Sharma ◽  
Manish Mahawar ◽  
...  

AbstractSalmonella enterica serovar Gallinarum is a host-restricted bacterial pathogen that causes a serious systemic disease exclusively in birds of all ages. Salmonella enterica serovar Typhimurium is a host-generalist serovar. Dendritic cells (DCs) are key antigen-presenting cells that play an important part in Salmonella host-restriction. We evaluated the differential response of chicken blood monocyte-derived dendritic cells (chMoDCs) exposed to S. Gallinarum or S. Typhimurium. S. Typhimurium was found to be more invasive while S. Gallinarum was more cytotoxic at the early phase of infection and later showed higher resistance against chMoDCs killing. S. Typhimurium promoted relatively higher upregulation of costimulatory and other immune function genes on chMoDCs in comparison to S. Gallinarum during early phase of infection (6 h) as analyzed by real-time PCR. Both Salmonella serovars strongly upregulated the proinflammatory transcripts, however, quantum was relatively narrower with S. Gallinarum. S. Typhimurium-infected chMoDCs promoted relatively higher proliferation of naïve T-cells in comparison to S. Gallinarum as assessed by mixed lymphocyte reaction. Our findings indicated that host restriction of S. Gallinarum to chicken is linked with its profound ability to interfere the DCs function. Present findings provide a valuable roadmap for future work aimed at improved vaccine strategies against this pathogen.


Author(s):  
Gökhan CENGİZ ◽  
Aslı ŞAHİNER ◽  
Evren ALGIN YAPAR ◽  
Bilge Ahsen KARA ◽  
Rakesh Kumar Sindhu

Recent serious effects of viral infections on humans, anti-viral compound research has gained momentum and has become very important. In particular, viruses such as Cytomegalovirus/CMV, Epstein Barr virus/EBV, Hepatitis B/HBV, Hepatitis C/HCV, Herpes Simplex Virus/HSV, Human Immunodeficiency Virus/HIV, rabies virus, corona virus and Ebola virus are of high importance. Very limited antiviral medicines commercially available and can cause serious-significant side effects for patients receiving treatment. Also, viruses have the mutational capability to infect host cells. For this reason, in recent years, the possibility of producing new antiviral medicines, especially from natural sources, has increased considerably, and animal-based products are now also promising among natural products. Viral-borne infections have been known long ago. However, it was just possible to isolate these viruses that cause infection in the nineteenth century. The management of viral infection, the isolation of the virus, and the control of viral reproduction has played a role in the development of many drugs and vaccines in the studies carried out since that period. In addition to these studies, viruses continue to be one of the primary causes of human and animal diseases today. It has been stated that Antiviral Peptides/AVPs can be used as a defense barrier with previous studies. Some AVPs are known to show a broad spectrum against viruses. In this direction, many studies have been conducted on AVPs and it has been observed that these peptides inhibit the viral particle by the above-mentioned mechanisms. In this study venoms and toxins of some animals, which have antiviral activities are overviewed.                    Peer Review History: Received 24 March 2021; Revised 15 April; Accepted 28 April, Available online 15 May 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Dr. Asia Selman Abdullah,  Al-Razi university, Department of Pharmacy, Yemen, [email protected] Dr. Nuray Arı, Ankara University, Turkiye, [email protected] Dr. Terhemen Festus Swem, Department of Veterinary Physiology and Biochemistry, College of Veterinary Medicine, Federal University of Agriculture, Makurdi, Benue State, Nigeria, [email protected] Similar Articles: EVALUATION OF ANTIBACTERIAL RESISTANCE OF BIOFILM FORMS OF AVIAN SALMONELLA GALLINARUM TO FLUOROQUINOLONES


2021 ◽  
Vol 8 (5) ◽  
pp. 71
Author(s):  
A. K. M. Ziaul Haque ◽  
Mir Rowshan Akter ◽  
SK Shaheenur Islam ◽  
Jahangir Alam ◽  
Sucharit Basu Neogi ◽  
...  

Salmonella Gallinarum is one of the most important bacterial pathogens associated with diminished egg production in poultry. The aim of this study was to understand the occurrence, molecular traits and antimicrobial resistance patterns of Salmonella Gallinarum strains isolated from small-scale commercial layer flocks with low level biosecurity standards in Bangladesh. A total of 765 samples, including cloacal swabs (535), visceral organs (50), and droppings (180), were collected from chickens of 12 layer flocks in 11 districts. Salmonella Gallinarum was isolated and characterized through culture-based method, followed by biochemical tests, sero-grouping, PCR assays, sequencing, and antibiogram. The identity of biochemically detected isolates of Salmonella Gallinarum was confirmed via genus-specific 16S rRNA gene based PCR, followed by invA and spvC genes based PCR assays. Occurrence of Salmonella Gallinarum was detected in overall 25.75% (197/765) samples, with a significantly (p < 0.05) higher incidence in visceral organs (42%) in comparison to cloacal swab (24%) and droppings (26%). Sequencing and subsequent phylogenetic analysis of invA and spvC genes in representative strains of Salmonella Gallinarum revealed a close genetic lineage, with a sequence similarity of 98.05–99.21% and 97.51–99.45%, respectively, to previously published sequences of the corresponding genes from the same serogroup strains. Remarkably, 66.5% (131/197) of the isolated strains of Salmonella Gallinarum were found to be resistant to 3 to 6 antimicrobial agents, and interpreted as multidrug resistant (MDR). The findings of this study underscore an inherent need of appropriate control measures to curb the widespread incidence of MDR Salmonella Gallinarum in small-scale commercial layer flocks, thereby, facilitating enhanced egg production and further support to the food security and safety in low resource settings.


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