scholarly journals Functional interaction between Mus81-Mms4 and Rad52 in Saccharomyces cerevisiae

2019 ◽  
Vol 14 (9) ◽  
pp. 122
Author(s):  
Phung Thi Thu Huong ◽  
Tran Hong Diem ◽  
Nguyen Luong Hieu Hoa ◽  
Vo Thanh Sang ◽  
Le Van Minh ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Homologous Recombination ◽  
Dna Sequence ◽  
Homologous Recombination Repair ◽  
Replication Forks ◽  
Functional Interaction ◽  
Dna Structures ◽  
Homology Searching ◽  
Recombination Repair ◽  
Species Specific

Mus81-Mms4 is a well conserved DNA structure–specific endonuclease and efficiently cleaves different DNA structures that could arise during the repair of stalled/blocked replication forks and homologous recombination repair. Rad52 is an ezyme that stimulates main steps of  DNA sequence-homology searching. In this study, we proved that Rad52 and Mus81-Mms4 possess a species-specific functional interaction, indicating that Rad52 and Mus81-Mms4 collaborate in processing of homologous recombination intermediates.

scholarly journals Spatiotemporal dynamics of homologous recombination repair at single collapsed replication forks

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Donna R. Whelan ◽  
Wei Ting C. Lee ◽  
Yandong Yin ◽  
Dylan M. Ofri ◽  
Keria Bermudez-Hernandez ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Spatiotemporal Dynamics ◽  
Homologous Recombination Repair ◽  
Replication Forks ◽  
Recombination Repair

scholarly journals Shu Proteins Promote the Formation of Homologous Recombination Intermediates That Are Processed by Sgs1-Rmi1-Top3

2007 ◽  
Vol 18 (10) ◽  
pp. 4062-4073 ◽  
Author(s):  
Hocine W. Mankouri ◽  
Hien-Ping Ngo ◽  
Ian D. Hickson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Homologous Recombination ◽  
S Phase ◽  
Specific Role ◽  
Homologous Recombination Repair ◽  
Recombination Repair ◽  
Mutant Phenotypes ◽  
Precise Role

CSM2, PSY3, SHU1, and SHU2 (collectively referred to as the SHU genes) were identified in Saccharomyces cerevisiae as four genes in the same epistasis group that suppress various sgs1 and top3 mutant phenotypes when mutated. Although the SHU genes have been implicated in homologous recombination repair (HRR), their precise role(s) within this pathway remains poorly understood. Here, we have identified a specific role for the Shu proteins in a Rad51/Rad54-dependent HRR pathway(s) to repair MMS-induced lesions during S-phase. We show that, although mutation of RAD51 or RAD54 prevented the formation of MMS-induced HRR intermediates (X-molecules) arising during replication in sgs1 cells, mutation of SHU genes attenuated the level of these structures. Similar findings were also observed in shu1 cells in which Rmi1 or Top3 function was impaired. We propose a model in which the Shu proteins act in HRR to promote the formation of HRR intermediates that are processed by the Sgs1-Rmi1-Top3 complex.

scholarly journals EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair

PLoS Genetics ◽  
2015 ◽  
Vol 11 (12) ◽  
pp. e1005675 ◽  
Author(s):  
Yuehan Wu ◽  
Suk-Hee Lee ◽  
Elizabeth A. Williamson ◽  
Brian L. Reinert ◽  
Ju Hwan Cho ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Genome Stability ◽  
Homologous Recombination Repair ◽  
Replication Forks ◽  
Recombination Repair ◽  
End Resection

Interactions of the HSM3 gene with genes initiating homologous recombination repair in yeast Saccharomyces cerevisiae

2012 ◽  
Vol 48 (3) ◽  
pp. 284-290 ◽  
Author(s):  
A. Yu. Chernenkov ◽  
D. V. Fedorov ◽  
L. M. Gracheva ◽  
T. A. Evstuhina ◽  
S. V. Kovaltsova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Homologous Recombination ◽  
Homologous Recombination Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Recombination Repair

scholarly journals A physiological significance of the functional interaction between Mus81 and Rad27 in homologous recombination repair

2015 ◽  
Vol 43 (3) ◽  
pp. 1684-1699 ◽  
Author(s):  
Huong Phung Thi Thu ◽  
Tuan Anh Nguyen ◽  
Palinda Ruvan Munashingha ◽  
Buki Kwon ◽  
Quy Dao Van ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Physiological Significance ◽  
Homologous Recombination Repair ◽  
Functional Interaction ◽  
Recombination Repair

scholarly journals Deficient SUMO Attachment to Flp Recombinase Leads to Homologous Recombination-dependent Hyperamplification of the Yeast 2 μm Circle Plasmid

2009 ◽  
Vol 20 (4) ◽  
pp. 1241-1251 ◽  
Author(s):  
Ling Xiong ◽  
Xiaole L. Chen ◽  
Hannah R. Silver ◽  
Noreen T. Ahmed ◽  
Erica S. Johnson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Homologous Recombination ◽  
Homologous Recombination Repair ◽  
Secondary Effects ◽  
Rolling Circle ◽  
Flp Recombinase ◽  
Recombination Repair ◽  
Sumo Pathway ◽  
Break Induced Replication

Many Saccharomyces cerevisiae mutants defective in the SUMO pathway accumulate elevated levels of the native 2 μm circle plasmid (2 μm). Here we show that accumulation of 2 μm in the SUMO pathway mutants siz1Δ siz2Δ, slx5Δ, and slx8Δ is associated with formation of an aberrant high-molecular-weight (HMW) form of 2 μm. Characterization of this species from siz1Δ siz2Δ showed that it contains tandem copies of the 2 μm sequence as well as single-stranded DNA. Accumulation of this species requires both the 2 μm–encoded Flp recombinase and the cellular homologous recombination repair (HRR) pathway. Importantly, reduced SUMO attachment to Flp is sufficient to induce formation of this species. Our data suggest a model in which Flp that cannot be sumoylated causes DNA damage, whose repair via HRR produces an intermediate that generates tandem copies of the 2 μm sequence. This intermediate may be a rolling circle formed via break-induced replication (BIR), because mutants defective in BIR contain reduced levels of the HMW form. This work also illustrates the importance of using cir° strains when studying mutants that affect the yeast SUMO pathway, to avoid confusing direct functions of the SUMO pathway with secondary effects of 2 μm amplification.

scholarly journals The possible function of Flp1 in homologous recombination repair in Saccharomyces cerevisiae

AIMS Genetics ◽  
2018 ◽  
Vol 5 (2) ◽  
pp. 161-176
Author(s):  
Huong Thi Thu Phung ◽  
◽  
Hoa Luong Hieu Nguyen ◽  
Dung Hoang Nguyen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Homologous Recombination ◽  
Homologous Recombination Repair ◽  
Recombination Repair
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