scholarly journals The determination of specific phosphatmobilizing activity of soil microorganisms

2021 ◽  
pp. 43-47
Author(s):  
I.M. Malynovska
Keyword(s):  
Liquid Medium ◽  
Specific Activity ◽  
Agar Medium ◽  
Correlation Coefficients ◽  
Culture Fluid ◽  
Close Correlation ◽  
Genus Bacillus ◽  
Dissolved Phosphorus ◽  
Average Value ◽  
Microbiological Laboratory

The study aimed to compare the phosphate-mobilizing activity of 69 strains of the genus Bacillus on agar and liquid Muromtsev medium to identify a possible correlation between them. Research methods: microbiological, laboratory-analytical, and statistical. The main results of the study. The closeness of the relationships between the activity of phosphates dissolving on agar and liquid nutrient media by strains of the genus Bacillus have been determined. There is no significant correlation between the size of the dissolution zone on the agar medium and the phosphorus removal in the culture fluid in a liquid medium has been found. However, the correlation coefficients between specific indicators - specific phosphate-mobilizing activity in a liquid nutrient medium (the ratio of the total quantity of dissolved phosphorus to the amount of grown biomass) and specific phosphamobilizing activity on agar medium (the ratio of the zone dimensions of dissolution to the colony size) - is considerable: for the first group = 0.603; for the second group R = 0.752; for the third group R = 0.864; for the fifth group, R = 0.732. The correlation coefficient has an average value (0.415) for the fourth group, includes strains with high phosphate-mobilizing activity (museum strains B. subtilis 54, B. subtilis 72, B. subtilis 101s, B. subtilis 100), as well a new isolated from the soil strains of Bacillus sp. D-31 and D-32. There is a close correlation (R3 = 0,670) between two specific indicators: specific phosphate-mobilizing activity on agar medium (Kr) and specific activity of FMP dissolution in liquid medium (Asp.). (Analysis has conducted taking into account all investigated strains of bacilli). Conclusions. The new indicator of specific phosphate-mobilizing activity use will simplify the screening of strains on agar medium.

Rates of de novo Synthesis of Malate Synthase and Albumins during the Very Early Phase of Germination

1979 ◽  
Vol 34 (12) ◽  
pp. 1237-1242 ◽  
Author(s):  
Wolfram Köller ◽  
Helmut Kindl
Keyword(s):  
Early Phase ◽  
De Novo ◽  
Specific Activity ◽  
Specific Radioactivity ◽  
Malate Synthase ◽  
Polyacrylamide Gel ◽  
De Novo Synthesis ◽  
Albumin Fraction ◽  
Average Value ◽  
Large Pool

Abstract Malate synthase is synthesized de novo in the very early phase of germination. Its molecular and immunological properties do not differ from those of malate synthase from fully developed cotyledons. Radioactive leucine was administered to dry seeds of cucumber, and its incorporation into proteins of cotyledons was examined after 2 days of germination. The specific radioactivity of malate synthase, purified by immunoprecipitation and electrophoresis on polyacrylamide gel, was only 1/20 the average value of the total albumin fraction. The minimal incorporation documented by the comparatively low specific activity of isolated malate synthase is discussed in relation to the large pool of malate synthase already present in dry seeds.

scholarly journals EXPRESSION OF b-XYLOSIDASE ENCODING GENE IN PHIS/ Bacillus megaterium MS SYSTEM

2015 ◽  
Vol 2 (1) ◽  
pp. 25 ◽  
Author(s):  
Sri Sumarsih
Keyword(s):  
Culture Medium ◽  
Culture Supernatant ◽  
Specific Activity ◽  
Agar Medium ◽  
Nitrilotriacetic Acid ◽  
Protoplast Transformation ◽  
E Coli ◽  
Recombinant Cells ◽  
Relative Molecular Weight ◽  
Encoding Gene

b-Xylosidase encoding gene from G. thermoleovorans IT-08 had been expressed in the pHIS1525/ B. megaterium MS941 system. The b-xylosidase gene (xyl) was inserted into plasmid pHIS1525 and propagated in E. coli DH10b. The recombinant plasmid was transformed into B. megaterium MS941 by protoplast transformation. Transformants were selected by growing the recombinant cells on solid LB medium containing tetracycline (10 µg/ ml). The expression of the b-xylosidase gene was assayed by overlaid the recombinant B. megaterium MS941 cell with agar medium containing 0.2% ethylumbelliferyl-b-D-xyloside (MUX). This research showed that the b-xylosidase gene was succesfully sub-cloned in pHIS1525 system and expressed by the recombinant B. megaterium MS941. Theaddition of 0.5% xylose into the culture medium could increase the activity of recombinantactivity of recombinant of recombinantb-xylosidase by 2.74 fold. The recombinant B. megaterium MS941 secreted 75.56% of the expressed b-xylosidase into culture medium. The crude extract b-xylosidase showed the optimum activity at 50° C and pH 6. The recombinant b-xylosidase was purified from culture supernatant by affinity chromatographic method using agarose containing Ni-NTA (Nickel-Nitrilotriacetic acid). The pure b-xylosidase showed a specific activity of 10.06 Unit/mg protein and relative molecular weight ± 58 kDa.

scholarly journals INVESTIGATION OF RELATIONSHIP BETWEEN PINE (PINUS SYLVESTRIS L.) BIOMASS AND 137CS SPECIFIC ACTIVITY IN WOOD

2006 ◽  
Vol 14 (3) ◽  
pp. 135-140 ◽  
Author(s):  
Donatas Butkus ◽  
Ingrida Pliopaitė Bataitienė
Keyword(s):  
Power Plant ◽  
Pinus Sylvestris ◽  
Nuclear Power ◽  
Specific Activity ◽  
Chernobyl Nuclear Power Plant ◽  
Biomass Growth ◽  
Annual Rings ◽  
Global Pollution ◽  
Nuclear Explosions ◽  
Average Value

The paper analyses relationship between pine (Pinus sylvestris L.) biomass and the specific activity of an artificial radio‐isotope 137Cs in wood. The specific activity of 137Cs was measured in the segments of pine (Pinus sylvestris L.) annual rings. The biomass of segments and the density of wood were estimated experimentally. It is determined that relationship between pine (Pinus sylvestris L.) biomass and pine age is not linear. In the variation of biomass are time when biomass grows slowly (25–55 pine age or 1959–1990 m. period). The specific activity of 137Cs in pine annual rings depends on the rapidity of biomass growth. The specific activity of 137Cs varied from 0,3 ± 0,1 to 13,4 ± 2,7 Bq/kg when the biomass grew rapidly and its average value was 4,3±1,1 Bq/kg. When the biomass grew slowly, the specific activity of 137Cs varied from 1,8 ± 0,7 to 20,6 ± 5,3 Bq/kg, and its average value was 9,6 ± 2,6 Bq/kg. Global pollution after nuclear explosions, pollution after the Chernobyl Nuclear Power Plant accident and uptake of 137Cs from roots had an influence on the specific activity of 137Cs in pine annual rings.

Analysis of trigonometric chaotic sequence by proposing an index-based bit level scrambling image encryption

2021 ◽  
pp. 2150406
Author(s):  
Wanbo Yu ◽  
Hao Wang
Keyword(s):  
Chaotic Map ◽  
Three Dimensional ◽  
Correlation Coefficients ◽  
Chaotic Sequence ◽  
Binary Number ◽  
Encryption Scheme ◽  
Binary Matrix ◽  
Binary Images ◽  
Average Value ◽  
Research Fields

Chaotic map is applied to numerous research fields, such as encryption of data and information. In this paper, a novel bit-level scrambling encryption based on three-dimensional trigonometric chaotic sequence is proposed. This encryption is operated on grayscale images because pixel value of which is between 0 and 255, which can be converted into 8-bit binary number, then each image can be converted into eight binary images. Combine binary images a three-dimensional matrix as input, which have three index values width, height, and page. Generate three numbers by the chaotic sequence to locate the value of the matrix need to be changed. After the value is located, change its value 0 to 1 or 1 to 0, this is the basic encryption scheme. This paper makes a three-dimensional binary matrix with 32 images to test the encryption scheme, and get average value of images’ number of pixel change rate at 0.9603, unified averaged change intensity at 31.27%, information entropy at 7.9891, also the histograms. And correlation coefficients of each pixel from three directions are small. There are comparisons with other encryptions too. The key space of this encryption is more than 10[Formula: see text].

scholarly journals Studies on the Rain Scavenging Process of Tritium in a Tropical Site at Narora in India

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Y. P. Gautam ◽  
Saivajay Sharma ◽  
A. K. Sharma ◽  
Aviansh Kumar ◽  
P. M. Ravi ◽  
...  
Keyword(s):  
Wet Deposition ◽  
Activity Ratio ◽  
Specific Activity ◽  
Deposition Velocity ◽  
Ground Level ◽  
Atomic Power Station ◽  
Tropical Region ◽  
Power Station ◽  
Data Set ◽  
Average Value

This study presents the results of systematic experiments on tritium (3H) concentrations in ground level air against those in rainwater near a pressurized heavy water reactor in a tropical region. The samples were collected over the rainy season of year 2011 from eight locations in the environment around Narora Atomic Power Station. The specific activity ratio of 3H between rainwater and air moisture at ground level was calculated for each data set. The average specific activity ratio was found to be ranged from 0.12 to 1.1. A correlation ( to 0.76, ) was observed between the total rain hours in a day and the rainwater 3H activity. Higher rain duration with slower rain rate yielded higher 3H concentrations as more time was available for the scavenging/wash-out process to take effect together with lower dilution. Annual tritium (HTO) wet deposition has been measured and calculated for the year 2011 within 0.8 km distance from 145 m high stack of Narora Atomic Power Station (NAPS) at nine locations in different directions. The range of deposition velocity, (m·s−1), at nine locations for the years 2011 is found to be from 4.43E − 04 to 6.42E − 03. The average value for wet deposition velocity for NAPS site is estimated as 3.17E − 03 m·s−1.

Adaptation of actomyosin ATPase in different types of muscle to endurance exercise

1975 ◽  
Vol 229 (2) ◽  
pp. 422-426 ◽  
Author(s):  
KM Baldwin ◽  
WW Winder ◽  
JO Holloszy
Keyword(s):  
Atpase Activity ◽  
Specific Activity ◽  
Vastus Lateralis ◽  
Close Correlation ◽  
Treadmill Running ◽  
Vastus Lateralis Muscle ◽  
Actomyosin Atpase ◽  
Different Types ◽  
Phosphofructokinase Activity ◽  
Fast Twitch

Higher concentrations of actomyosin were found in the red portion of the vastus lateralis and in the white portion of the vastus lateralis muscle than in the soleus or heart in rats. A strenuous program of treadmill running lasting 18 wk or longer did not significantly affect the amount of actomyosin recovered from the different types of muscle. No changes in actomyosin ATPase occurred in fast-twitch white (white vastus) or heart muscles in response to the exercise training. In contrast, a decrease of approximately 20% occurred in the specific activity of actomyosin ATPase of fast-twitch red (red vastus) muscle (0.635 +/- 0.029 mumol Pi/min per milligram for sedentary vs. 0.529 +/- 0.021 mumol Pi/min per milligram for trained), while the actomyosin ATPase activity of slow-twitch red (soleus) muscle increased about 20% (0.209 +/- 0.033 vs. 0.257 +/- 0.031 mumol Pi/min per milligram). There was a close correlation (r = 0.99, P less than 0.001) between actomyosin ATPase activity and phosphofructokinase activity in the three types of skeletal muscles and in heart muscle of exercise-trained and untrained animals, providing further evidence in support of the concept that the glycogenolytic capacity of a muscle and its actomyosin ATPase activity are regulated in parallel.

Enumeration of petroleum-degrading marine and estuarine microorganisms by the most probable number method

1978 ◽  
Vol 24 (5) ◽  
pp. 552-557 ◽  
Author(s):  
A. L. Mills ◽  
C. Breuil ◽  
R. R. Colwell
Keyword(s):  
Crude Oil ◽  
Liquid Medium ◽  
Silica Gel ◽  
Agar Medium ◽  
Most Probable Number ◽  
Probable Number ◽  
Petroleum Degraders

Several media designed for use in a most probable number (MPN) determination of petroleum-degrading microorganisms were compared. The best results, i.e., largest numbers, were obtained using a buffered (32 mM PO4≡) liquid medium containing 1% hydrocarbon substrate. Of 104 presumptive oil degraders tested, 20 grew on oil agar medium but did not utilize oil or a mixture of pure paraffinic hydrocarbons (C10 to C16n-alkanes) in liquid (MPN) medium. Visible turbidity in the liquid medium was correlated with hydrocarbon utilization. Counts of petroleum degraders obtained using liquid medium (MPN) were in most cases higher than those obtained on an oil-amended silica gel medium. Both procedures yield an estimation of oil degraders, and the oil-amended agar permits growth of organisms which do not degrade crude oil. All strains of oil-degrading microorganisms examined in this study were lipolytic, but the converse was not always true.

scholarly journals Exo-β-glucanases in yeast

1968 ◽  
Vol 109 (3) ◽  
pp. 347-360 ◽  
Author(s):  
Ahmed T. H. Abd-El-Al ◽  
H. J. Phaff
Keyword(s):  
Specific Activity ◽  
Culture Fluid ◽  
Competitive Inhibitor ◽  
Cell Extract ◽  
Baker’S Yeast ◽  
Maximal Velocity ◽  
Baker's Yeast ◽  
Molecular Weights ◽  
Hansenula Anomala ◽  
Single Enzyme

1. A number of yeast species were examined for the presence of β-glucanases. Extracts obtained by cell disruption of Saccharomyces cerevisiae, Fabospora fragilis and Hansenula anomala hydrolysed laminarin and pustulan with the production of glucose. Enzymic activities were also detected in the culture fluids of F. fragilis and H. anomala grown aerobically in buffered mineral medium with glucose as the carbon source. 2. F. fragilis and H. anomala possessed approximately sevenfold higher β-(1→3)-glucanase activity than S. cerevisiae. 3. Intracellular exo-β-glucanase from baker's yeast was purified 344-fold from the dialysed cell extract. 4. Exo-β-glucanase from F. fragilis was purified 114-fold from the dialysed culture fluid and 423-fold from the dialysed intracellular extract. The purified extracellular and intracellular enzymes had similar properties and essentially the same specific activity, 79 enzyme units/mg. of protein. 5. Extracellular exo-β-glucanase of H. anomala was purified 600-fold. 6. The optimum pH of the enzymes from F. fragilis, S. cerevisiae and H. anomala was 5·5 in each case. Chromatographic evidence indicated that the three enzymes remove glucosyl units sequentially from laminarin as well as pustulan. 7. The ratio of activities towards laminarin and pustulan remained constant during purification of the exo-β-glucanase obtained from the three species, suggesting a single enzyme. Additional evidence for its unienzymic nature are: (i) the two activities were destroyed at exactly the same rate on heating of the purified enzyme from F. fragilis at three different temperatures; (ii) the competitive inhibitor glucono-δ-lactone gave the same value of Ki when tested with either substrate; (iii) quantitative application of the ‘mixed-substrate’ method with the purified enzyme of S. cerevisiae gave data that were in excellent agreement with those calculated on the assumption of a single enzyme. 8. The purified exo-β-glucanases of the different species of yeast had different kinetic constants. The ratios of maximal velocities and Km values with laminarin and pustulan differed markedly. Comparison of Vmax. and Km values suggests that the rapid release of spores from asci in F. fragilis might be explained in terms of an enzyme with higher maximal velocity and higher affinity to the ascus wall than that present in baker's yeast. 9. The estimated molecular weights for exo-β-glucanases from F. fragilis, S. cerevisiae and H. anomala were 22000, 40000 and 30000 respectively.

scholarly journals An Efficient Method of Pennisetum x Advena ‘Rubrum’ Seedlings Production Using Temporary Immersion Bioreactor and Agar Cultures

2021 ◽  
Author(s):  
Mariusz Pożoga ◽  
Dawid Olewnicki ◽  
Elżbieta Wójcik-Gront
Keyword(s):  
Liquid Medium ◽  
Efficient Method ◽  
Activated Charcoal ◽  
Agar Medium ◽  
Ms Medium ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bright Green ◽  
Bioreactor System

Abstract The aim of this study was to propose an efficient method of Pennisetum x advena ‘Rubrum’ micropropagation. Agar cultures with MS medium supplemented with BAP in various concentrations (0.5 mg/L-2 mg/L) and a temporary immersion bioreactor system (TIS) with liquid medium MS with an addition of 1 mg/L BAP were used. For rooting ½ MS medium with different auxin combinations (IBA, NAA) and activated charcoal was utilized. The most efficient method turned out to be TIS which produced 36.9 new plants in four weeks. The seedlings were slender in shape, bright green in colour with no signs of hyperhydricity. The most suitable agar medium produced 19.5 new plants in an eight week period. Rooting should be carried on ½ MS supplemented with 0.5 mg/L IBA and 0.5 mg/L NAA with an 84% rooting rate. The addition of activated charcoal inhibited rooting.

scholarly journals Regression analysis of expanded polystyrene properties

2020 ◽  
Vol 6 (2) ◽  
pp. 54-63
Author(s):  
Dušan Páleš ◽  
Milada Balková
Keyword(s):  
Regression Models ◽  
Rank Correlation ◽  
Correlation Coefficients ◽  
Close Correlation ◽  
Expanded Polystyrene ◽  
Ease Of Use ◽  
Exponential Form ◽  
Point Estimates ◽  
Linear Polynomial ◽  
Pearson Coefficient

Own measurements examine the tensile strength of expanded polystyrene (EPS) depending on its bulk density. 30 samples were used to calculate the correlation coefficients between these two properties. In addition to the standard Pearson coefficient, we also calculate the rank correlation coefficients, Spearman´s and Kendall´s. By testing the hypotheses, we verify the correlation of the entire population. After finding a relatively close correlation (0.6 - 0.8), we apply different regression models, especially polynomial, but also exponential. We evaluate the properties of parameters in models, their point estimates and confidence intervals. Based on the characteristics of each of the seven regressions, we found the best exponential form of the dependence, before the linear polynomial. The complexity of a mathematical model does not always mean that it is also a more accurate approximation. On the other hand, a simple model makes it possible, in addition to its ease of use, to more closely reflect the examined dependence.

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