An Efficient Method of Pennisetum x Advena ‘Rubrum’ Seedlings Production Using Temporary Immersion Bioreactor and Agar Cultures

Temporary Immersion ◽

Bright Green ◽

Abstract The aim of this study was to propose an efficient method of Pennisetum x advena ‘Rubrum’ micropropagation. Agar cultures with MS medium supplemented with BAP in various concentrations (0.5 mg/L-2 mg/L) and a temporary immersion bioreactor system (TIS) with liquid medium MS with an addition of 1 mg/L BAP were used. For rooting ½ MS medium with different auxin combinations (IBA, NAA) and activated charcoal was utilized. The most efficient method turned out to be TIS which produced 36.9 new plants in four weeks. The seedlings were slender in shape, bright green in colour with no signs of hyperhydricity. The most suitable agar medium produced 19.5 new plants in an eight week period. Rooting should be carried on ½ MS supplemented with 0.5 mg/L IBA and 0.5 mg/L NAA with an 84% rooting rate. The addition of activated charcoal inhibited rooting.

Micropropagation of pineapple (Ananas comosus L. Var. Smooth cayenne) in temporary immersion bioreactor system (TIPS)

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v12i2.29 ◽

2021 ◽

Vol 12 (2) ◽

pp. 207-209

Author(s):

L.A. Sani ◽

I.S. Usman ◽

A.U. Nasir ◽

M.M. Abdulmalik

Keyword(s):

Activated Charcoal ◽

Ananas Comosus ◽

Multiplication Rate ◽

Temporary Immersion System ◽

Temporary Immersion ◽

The Family ◽

Root Morphogenesis ◽

Bioreactor System ◽

Planting Materials

Pineapple is an important edible fruit in the family Bromeliaceae popularly grown in the tropical and subtropical countries. Commercial prodution of pineapple requires large volume of planting materials which could not easily be obtained using conventional method of propagation. A protocol for mass propagation of pineapple (Ananas comosus L. var.smooth cayenne) using temporary immersion bioreactor system has been developed.The protocol involves four immersion cycles in Murashinge and Skoog (MS) media fortified with 1mg/L or 2mg/L 6-Benzylaminopurine (BAP) with or without 0.25g/L activated charcoal (AC). The highest multiplication rate (120 -130 plants/bottle) was obtained when media was fortified with 1mg/l or 2mg/L BAP alone. The presence of activated charcoal (AC) promoted root morphogenesis, resulting in significant increase in roots formation in BAP suplemented media. A combination of BAP with AC significantly increased the number of competent plants(20 – 30 plants/bottle) after four weeks of culture in temporary immersion system. The system is recommended for rapid and efficient micropropagation of pineapple.

Cannabis sativa L. Micropropagation in Temporary Immersion Bioreactor System

Planta Medica ◽

10.1055/s-0030-1251771 ◽

2010 ◽

Vol 76 (05) ◽

Cited By ~ 1

Author(s):

H Lata ◽

S Chandra ◽

IA Khan ◽

MA ElSohly

Keyword(s):

Cannabis Sativa ◽

Temporary Immersion ◽

MASS SHOOT PROLIFERATION OF PISTACIA KHINJUK STOCKS USING TEMPORARY IMMERSION BIOREACTOR SYSTEM (TIS)

Acta Horticulturae ◽

10.17660/actahortic.2014.1028.24 ◽

2014 ◽

pp. 145-151 ◽

Cited By ~ 1

Author(s):

E. Tilkat ◽

V. Süzerer ◽

Y. Ersali ◽

A. Hoșer ◽

F.M. Kilinç ◽

...

Keyword(s):

Shoot Proliferation ◽

Temporary Immersion ◽

Pistacia Khinjuk ◽

Effect of activated charcoal on multiplication of African yam (Dioscorea cayenensis-rotundata) nodal segments using a temporary immersion bioreactor (RITA®)

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-013-9552-6 ◽

2013 ◽

Vol 50 (2) ◽

pp. 210-216 ◽

Cited By ~ 7

Author(s):

Frederik Polzin ◽

Isabelle Sylvestre ◽

Eveline Déchamp ◽

Pascal Ilbert ◽

Hervé Etienne ◽

...

Keyword(s):

Activated Charcoal ◽

Nodal Segments ◽

Temporary Immersion ◽

Dioscorea Cayenensis

Generation of Genetically Modified Strawberries in Temporary Immersion Bioreactor System

Biotechnology and Sustainable Agriculture 2006 and Beyond ◽

10.1007/978-1-4020-6635-1_13 ◽

2007 ◽

pp. 115-118

Author(s):

Kati Hanhineva ◽

Harri Kokko ◽

Sirpa Karenlampi

Keyword(s):

Genetically Modified ◽

Temporary Immersion ◽

Efficient production of virus-free apple plantlets using the temporary immersion bioreactor system

Horticulture Environment and Biotechnology ◽

10.1007/s13580-020-00257-3 ◽

2020 ◽

Vol 61 (4) ◽

pp. 779-785

Author(s):

Na-Young Kim ◽

Ho-Dong Hwang ◽

Jin-Ho Kim ◽

Bo-Min Kwon ◽

Daeil Kim ◽

...

Keyword(s):

Efficient Production ◽

Temporary Immersion ◽

A Pattern-Based Approach for Detecting Pneumatic Failures on Temporary Immersion Bioreactors

Sensors ◽

10.3390/s19020414 ◽

2019 ◽

Vol 19 (2) ◽

pp. 414 ◽

Cited By ~ 6

Author(s):

Octavio Loyola-González ◽

Miguel Medina-Pérez ◽

Dayton Hernández-Tamayo ◽

Raúl Monroy ◽

Jesús Carrasco-Ochoa ◽

...

Keyword(s):

Production Cost ◽

Biological Process ◽

Feature Representation ◽

Pneumatic System ◽

Temporary Immersion ◽

System A ◽

Contrast Pattern ◽

Bioreactor System ◽

Feature Values

Temporary Immersion Bioreactors (TIBs) are used for increasing plant quality and plant multiplication rates. These TIBs are actioned by mean of a pneumatic system. A failure in the pneumatic system could produce severe damages into the TIB. Consequently, the whole biological process would be aborted, increasing the production cost. Therefore, an important task is to detect failures on a temporary immersion bioreactor system. In this paper, we propose to approach this task using a contrast pattern based classifier. We show that our proposal, for detecting pneumatic failures in a TIB, outperforms other approaches reported in the literature. In addition, we introduce a feature representation based on the differences among feature values. Additionally, we collected a new pineapple micropropagation database for detecting four new types of pneumatic failures on TIBs. Finally, we provide an analysis of our experimental results together with experts in both biotechnology and pneumatic devices.