Micronucleus Test of DHU001, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice

Cylindrospermopsin (CYN) is an alkaloid commonly produced by some cyanobacteria that has been implicated in outbreaks of human illness. The aim of this study was to investigate the genotoxicity of Cylindrospermopsis raciborskii cellular content (including CYN) and its byproducts resulting from chlorination during water treatment. DNA damage in blood and liver cells was analysed by the comet assay and micronucleus test (MN). Mice were injected intraperitoneally with the following treatments: (a) physiological saline, (b) treated water, (c) treated water plus C. raciborskii extract (CYN producer strain, CYPO-011 K), (d) C. raciborskii extract (CYN producer strain, CYPO-011 K), (e) C. raciborskii extract (CYN non producer strain), and (f) treated water plus C. raciborskii extract (CYN non producer strain) extract. After 48 h, samples were taken to perform tests (blood and liver cells to the comet assay and bone marrow to MN test). The CYPO-011 K had a genotoxic and mutagenic effects on liver and bone marrow cells. The group that received chlorine-treated water plus CYPO-011 K also exhibited genotoxic effects in the liver, as well as in the blood, and a mutagenic effect in blood marrow cells. The results emphasise the need of improving CYN monitoring in waters bodies in order to reduce the risk of human exposure.

Download Full-text

A study of micronucleus induction with isopropenyl benzene and trimellitic anhydride in bone marrow cells of Institute for Cancer Research (ICR) mice

Journal of Environmental Chemistry and Ecotoxicology ◽

10.5897/jece12.007 ◽

2012 ◽

Vol 4 (6) ◽

Author(s):

Kyung-Taek RIM

Keyword(s):

Bone Marrow ◽

Cancer Research ◽

Bone Marrow Cells ◽

Trimellitic Anhydride ◽

Icr Mice ◽

Micronucleus Induction ◽

Marrow Cells

Download Full-text

Evaluation of the mutagenic effect of the iodinated contrast medium Urografina® 292 using the micronucleus test in mouse bone marrow cells

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652013000200018 ◽

2013 ◽

Vol 85 (2) ◽

pp. 737-744 ◽

Cited By ~ 1

Author(s):

MONICA B.B. BELLE ◽

DANIELA D. LEFFA ◽

DALIANE MAZZORANA ◽

VANESSA M. DE ANDRADE

Keyword(s):

Bone Marrow ◽

Contrast Media ◽

Contrast Medium ◽

Bone Marrow Cells ◽

Micronucleus Test ◽

Control Group ◽

Mouse Bone Marrow ◽

Iodinated Contrast ◽

Polychromatic Erythrocytes ◽

Marrow Cells

Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografina® 292 (meglumine amidotrizoate and sodium-ionic dimmer) in bone marrow cells (BMC) of mice in vivo. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina® 292 (3.0 mL/kg) presented an increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in relation at the control group (P<0.05). The results obtained after intraperitoneal administration of CM showed that all doses (1.0 mL/kg, 2.0 mL/kg and 3.0 mL/kg) increased the frequency of MNPCEs, being significantly different from the negative control (P< 0.01). The present results suggest that iodinated contrast media Urografina® 292 may cause a significant increase of cytogenetic damage in bone marrow cells of mice.

Download Full-text