scholarly journals Effect of auxin precursors and chemical analogues on the growth and chemical composition in Chlorella pyrenoidosa Chick

2014 ◽  
Vol 63 (3-4) ◽  
pp. 279-286 ◽  
Author(s):  
Romuald Czerpak ◽  
Andrzej Bajguz ◽  
Bożena Białecka ◽  
Lidia E. Wierzchołowska ◽  
Małgorzata M. Wolańska
Keyword(s):  
Acetic Acid ◽  
Anthranilic Acid ◽  
Phenylacetic Acid ◽  
Dry Weight ◽  
Chlorella Pyrenoidosa ◽  
Maximum Activity ◽  
Water Soluble ◽  
Dichlorophenoxyacetic Acid ◽  
Phenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

In this paper the authors present studies on the effect of auxin precursors and chemical analogues on the growth and biochemical composition in <i>Chlorella pyrenoidosa</i> (<i>Chlorophyceae</i>). Among auxin precursors tryptamine exhibited slightly higher stimulative activity in regard to fresh and dry weight, mineral substances, chlorophylls, carotenoids, monosaccharides (aldohexoses) and water-soluble proteins content in <i>Ch. pyrenoidosa</i> cells as compared to anthranilic acid. Among auxin analogues used phenoxyacetic acid and naphthyl-3-acetic acid had the strongest stimulative effect of the above-mentioned parameters. Their activity was significantly higher than that of auxin precursors. The activity of naphthyl-3-sulphonic acid was slightly lower than that of tryptamine, whereas the stimulation by 2,4-dichlorophenoxyacetic acid was similar to that of anthranilic acid. In <i>Ch. pyrenoidosa</i> cells 2,4-dichlorophenoxyacetic acid and naphthyl-3-sulphonic reached their maximum activity at the latest (between the 15th or 16th day) of the culturing, whereas tryptamine, phenylacetic acid, naphthyl-3-acetic acid and indolyl-3-acetic acid - at the earliest (between the 8th or 12th) day.

scholarly journals Changes in Leaf-Sugar Content and Enzyme Activity of Immature Sugarcane Following Foliar Application of Indole-3-Acetic Acid, 2,4-Dichlorophenoxyacetic Acid, and Maleic Hydrazide

1969 ◽  
Vol 49 (1) ◽  
pp. 1-34
Author(s):  
Alex G. Alexander
Keyword(s):  
Acetic Acid ◽  
Maleic Hydrazide ◽  
Foliar Application ◽  
Sugar Content ◽  
Water Soluble ◽  
Dichlorophenoxyacetic Acid ◽  
Enzyme Preparations ◽  
Freeze Dried ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

Indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid, and maleic hydrazide were applied as foliar sprays to 10-week-old sugarcane plants during initial studies of the interrelationships of growth-regulating materials with the sugar-metabolizing enzymes of sugarcane. Leaf samples were harvested at 1, 3, 9, and 27 days following treatment for sugar and enzyme assays. Sugar analyses were run for total ketoses, sucrose, fructose, and total reducing sugars, with glucose being determined by calculation. A series of acid phosphatase assays were conducted using as substrates the following compounds: ß-glycerophosphate, adenosinetriphosphate, uridine diphosphate glucose, glucose-1-phosphate, glucose-6-phosphate, fructose-6- phosphate, fructose-1,6-diphosphate, and 3-phosphoglyceric acid. Additional enzymes included invertase, amylase, hexokinase, phosphohexose isomerase, aldolase, triosephosphate dehydrogenase, phosphoglyceryl kinase, condensing enzyme, isocitric acid dehydrogenase, transaminase, peroxidase, and glucose oxidase. All enzyme preparations consisted of dialyzed water-soluble protein extracted from freeze-dried leaf tissue and precipitated with ammonium sulfate between 35 and 95 percent of saturation.

scholarly journals Response of pine hypocotyl sections to growth regulators and related substances

2015 ◽  
Vol 44 (1) ◽  
pp. 123-132
Author(s):  
J. Zakrzewski
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Growth Response ◽  
Extended Period ◽  
Dichlorophenoxyacetic Acid ◽  
Pinus Silvestris ◽  
Related Substances ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid ◽  
Synthetic Growth

Growth response of <i>Pinus silvestris</i> hypocotyl sections to some synthetic growth regulators and related substances was studied. Elongation of hypocotyl sections was stimulated by naphtaleneacetic acid, indole-3-acetic acid, in-dole-3-propionic acid, indole-3-butyric acid, 2,4-dichlorophenoxyacetic acid, indoleaoetic amide, indoleacetic nitrile and coumarin. Indole-3-acetic acid and naphtaleneacetic acid extended period of growth up to 16 and 24 hours, respectively. Growth was inhibited by kinetin, trans-cinnamic acid and 2,3,5-tri-iodobenzoic acid. No effect of gibberellic acid, tryptophan and biotin was observed.

Adsorption of 2,4-Dichlorophenoxyacetic Acid and Phenoxyacetic Acid on Sibunit

2018 ◽  
Vol 52 (1) ◽  
pp. 53-57 ◽  
Author(s):  
M. D. Vedenyapina ◽  
L. R. Sharifullina ◽  
S. A. Kulaishin ◽  
E. D. Strel’tsova ◽  
A. A. Vedenyapin ◽  
...  
Keyword(s):  
Dichlorophenoxyacetic Acid ◽  
Phenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

An improved culture medium for growing the orchid Cypripedium reginae axenically

1982 ◽  
Vol 60 (12) ◽  
pp. 2547-2555 ◽  
Author(s):  
Gaëtan Harvais
Keyword(s):  
Acetic Acid ◽  
Membrane Filtration ◽  
Ammonium Citrate ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Growth And Survival ◽  
Murashige Skoog ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Germination And Growth

A new medium for growing Cypripedium reginae Walt. axenically from seed was designed. Liquid culture proved unsuitable, hence a 1% agar medium supplemented with 5% potato extract was used to investigate optimal mineral element, vitamin, amino acid, sugar, and growth regulator supplements for germination, and subsequent growth. A modified Pfeffer solution with 1400 mg/L NH4NO3 + 19 mg/L ammonium citrate + 2% dextrose + 10 mg/L niacin + 5 mg/L calcium pantothenate + 5 mg/L thiamine HCl + 1 mg/L kinetin + 0.1 mg/L α-naphthaleneacetic acid gave best germination and growth to 2 years with little or no phenolic production. Gamborg's B5 medium and Murashige–Skoog (MS) medium were less than optimal when tested against the above medium. Growth regulators were more active when sterilized by membrane filtration instead of autoclaving. Of the three aminopurines tested, kinetin, benzylaminopurine (BAP), and 6(γ,γ-dimethylallylamino) purine (γγ), the order of activity was initially γγ → BAP → kinetin, but kinetin produced better greening of protocorms and plantlets, and eventually greater survival. Hence, it was chosen for further study. The auxins indole-3-acetic acid (IAA), naphthalene acetic acid (NAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D) were also tested alone and in combination with the aminopurines. They did not stimulate germination, but improved growth and survival when combined with aminopurines. The most active of the auxins were NAA → IAA → IBA → 2,4-D. A kinetin:NAA ratio of 10:1 was very satisfactory.

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