scholarly journals Transgenic Tall Fescue and Maize with Resistance to ALS-Inhibiting Herbicides

Author(s):  
Hiroko Sato ◽  
Tadashi Takamizo ◽  
Junko Horita ◽  
Kiyoshi Kawai ◽  
Koichiro Kaku ◽  
...  

Author(s):  
Zeng-Yu Wang ◽  
Andrew Hopkins ◽  
Robert Lawrence ◽  
Jeremey Bell ◽  
Megann Scott


2010 ◽  
Vol 32 (4) ◽  
pp. 577-577
Author(s):  
Kyung-Hee Kim ◽  
Iftekhar Alam ◽  
Ki-Won Lee ◽  
Shamima Akhtar Sharmin ◽  
Sang-Soo Kwak ◽  
...  


2003 ◽  
Vol 39 (3) ◽  
pp. 277-282 ◽  
Author(s):  
Z. Y. Wang ◽  
J. Bell ◽  
Y. X. Ge ◽  
D. Lehmann


2004 ◽  
Vol 23 (10-11) ◽  
pp. 705-709 ◽  
Author(s):  
Yuanlei Hu ◽  
Weilong Jia ◽  
Jundan Wang ◽  
Yanqin Zhang ◽  
Lili Yang ◽  
...  


2003 ◽  
Vol 107 (3) ◽  
pp. 406-412 ◽  
Author(s):  
Z. Y. Wang ◽  
M. Scott ◽  
J. Bell ◽  
A. Hopkins ◽  
D. Lehmann




HortScience ◽  
2009 ◽  
Vol 44 (5) ◽  
pp. 1254-1257 ◽  
Author(s):  
Hiroko Sato ◽  
Tadashi Takamizo ◽  
Tsutomu Shimizu ◽  
Kiyoshi Kawai ◽  
Koichiro Kaku

Herbicide-resistant turfgrass can be an efficient tool that will allow easier turf maintenance. Acetolactate synthase (ALS) is the first common enzyme in the biosynthetic pathways leading to the branched-chain amino acids, and amino acid substitutions in ALS have been known to confer resistance to ALS-inhibiting herbicides. A two-point mutated rice ALS gene [OsALS (dm)] has been shown to confer strong resistance to bispyribac-sodium (BS), an ALS-inhibiting herbicide. In this study, we introduced into turf-type tall fescue (Festuca arundinacea Schreb.) the OsALS (dm) gene by using Agrobacterium-mediated transformation for conferring herbicide resistance. Stable integration of the transgene was confirmed by Southern blot analysis. Transgenic and wild-type plants were sprayed on the leaves with herbicide containing BS; approximately half of the transgenic plants were unaffected by the treatment and showed resistance to the herbicide, whereas the wild-type plants died. ALS activity in the leaf tissue of transgenic-resistant plants incubated with BS was almost equivalent to that in wild-type plants without BS and was higher than in wild-type plants incubated with BS. These indicate that the transgenic-resistant plants actively produced OsALS (dm) protein under herbicide treatment. This is the first report of herbicide-resistant transgenic tall fescue after introduction of a mutated ALS gene.



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