scholarly journals Cytoplasmic Matrix and Viroplasms Inclusions in the Presence of Gold Nanoparticles (AuNPs)

2021 ◽  
Author(s):  
Noorah Abdulaziz Othman Alkubaisi ◽  
Nagwa Mohammed Amin Aref
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
High Concentration ◽  
Cytoplasmic Matrix ◽  
Virus Particles ◽  
Barley Yellow Dwarf ◽  
Treated Plant ◽  
Yellow Dwarf Virus

Cellular ultrastructure micrographs revealed striking changes resulting from the Barley Yellow Dwarf Virus (BYDV-PAV) infection in Electron microscopy. In the cytoplasm, the Gold nanoparticles (AuNPs) may bind with different cytoplasmic organelles and interfere with the treated site’s metabolic processes. The micrographs of the treated plant leave with AuNPs showing; Endosomes, amorphous bodies, slender filaments fibers, myelin bodies with a high concentration of virus particles, and Gold Nanoparticles distributed in a circulated shape in the cytoplasm with virus particles.

Detection of barley yellow dwarf virus in aphids by serologically specific electron microscopy

1982 ◽  
Vol 60 (2) ◽  
pp. 179-185 ◽  
Author(s):  
Y. C. Paliwal
Keyword(s):  
Electron Microscopy ◽  
Barley Yellow Dwarf Virus ◽  
Rhopalosiphum Padi ◽  
Virus Transmission ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Source Plant ◽  
Yellow Dwarf Virus ◽  
Good Agreement

A procedure employing serologically specific electron microscopy (SSEM) which consistently detected barley yellow dwarf virus (BYDV) in single aphids was developed. Using an antiserum against the Sitobion avenae (SA)-specific variant of BYDV, the virus was detected in aphids after 1 day of acquisition feeding and up to 9 days after removal from the virus source plant. The method was equally effective with alate or apterous aphids. SSEM scores of single aphids for presence of virus showed good agreement with virus transmission by aphids. In addition to the SA-specific variant, the nonspecific and Schizaphis graminum specific variants of the virus were also detected in their vectors Rhopalosiphum padi and S. graminum, respectively, using the antiserum to SA-specific variant. The SA-specific variant was also detected in aphids considered "nonvectors" (R. padi, R. maidis, and S. graminum) when fed on this variant. Application of the procedure for determining the proportion of viruliferous aphids in a population are discussed.

Sign in / Sign up

Export Citation Format

Share Document