scholarly journals Preimplantation Genetic Diagnosis for Beta Thalassemia

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Faravareh Khordadpoor Deilamani ◽  
Mohammad Taghi Akbari
Keyword(s):  
Preimplantation Genetic Diagnosis ◽  
Haplotype Analysis ◽  
Genetic Diagnosis ◽  
Severe Anaemia ◽  
Beta Thalassemia ◽  
Clinical Tool ◽  
Singleton Pregnancy ◽  
Cycle Sequencing ◽  
The Status ◽  
Variant Detection

Background: Beta thalassemia is an autosomal recessive genetic disease with the symptoms of severe anaemia, ineffective erythropoiesis and bone deformities. Preimplantation genetic diagnosis is a noninvasive clinical tool for couples who are at risk of affected pregnancy to have a healthy child. Objectives: Here we report a PGD test for a couple who were heterozygous for CD36/37(-T) mutation in HBB gene and had terminated one affected pregnancy. Methods: Haplotype analysis of 6 flanking STR markers as well as variant detection by cycle sequencing were included in our PGD test in order to investigate the status of the embryos reliably. Results: Three out of five embryos were transferable. Conclusions: One normal and one carrier embryo were transferred which resulted in the singleton pregnancy and the birth of a healthy girl.

scholarly journals First Report of Preimplantation Genetic Diagnosis for Steroid-Resistant Nephrotic Syndrome

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Faravareh Khordadpoor Deilamani ◽  
Mohammad Taghi Akbari
Keyword(s):  
Nephrotic Syndrome ◽  
Preimplantation Genetic Diagnosis ◽  
Genetic Diagnosis ◽  
Autosomal Recessive Inheritance ◽  
Cycle Sequencing ◽  
Steroid Resistant ◽  
Steroid Resistant Nephrotic Syndrome ◽  
Str Markers ◽  
The Status ◽  
Nphs2 Gene

Background: Steroid-resistant nephrotic syndrome is a genetic disease with autosomal recessive inheritance pattern and symptoms such as proteinuria and hypoalbuminemia and rapid progress of kidney disease. Preimplantation genetic diagnosis is an option for couples who are at risk of affected pregnancy to have a healthy child. Objectives: This study aimed to develop a new PGD test for a couple who are heterozygous for a mutation in NPHS2 gene and have a son affected to steroid-resistant nephrotic syndrome. Methods: Variant detection by cycle sequencing and Multiplex fluorescent PCR for identification of flanking STR markers were used to investigate the status of the embryos. Results: Three out of six embryos were transferable from which one was transferred and resulted in the birth of a healthy boy. Conclusions: We recommend increasing the number of the STR markers to two at the downstream of the NPHS2 gene especially in cases that direct mutation analysis such as cycle sequencing is not applied.

Successful preimplantation genetic diagnosis for alpha- and beta-thalassemia in China

2006 ◽  
Vol 26 (11) ◽  
pp. 1021-1028 ◽  
Author(s):  
Deng Jie ◽  
Peng Wen-Lin ◽  
Li Jie ◽  
Fang Cong ◽  
Liang Xiao-Yan ◽  
...  
Keyword(s):  
Preimplantation Genetic Diagnosis ◽  
Genetic Diagnosis ◽  
Beta Thalassemia

scholarly journals FMR1 CGG repeat expansion mutation detection and linked haplotype analysis for reliable and accurate preimplantation genetic diagnosis of fragile X syndrome

2017 ◽  
Vol 19 ◽  
Author(s):  
Indhu-Shree Rajan-Babu ◽  
Mulias Lian ◽  
Felicia S.H. Cheah ◽  
Min Chen ◽  
Arnold S.C. Tan ◽  
...  
Keyword(s):  
Fragile X Syndrome ◽  
Preimplantation Genetic Diagnosis ◽  
Haplotype Analysis ◽  
Fragile X ◽  
Genetic Diagnosis ◽  
In Vitro Fertilisation ◽  
Marker Haplotype ◽  
Cgg Repeat ◽  
Robust Detection

Fragile X mental retardation 1 (FMR1) full-mutation expansion causes fragile X syndrome. Trans-generational fragile X syndrome transmission can be avoided by preimplantation genetic diagnosis (PGD). We describe a robust PGD strategy that can be applied to virtually any couple at risk of transmitting fragile X syndrome. This novel strategy utilises whole-genome amplification, followed by triplet-primed polymerase chain reaction (TP-PCR) for robust detection of expanded FMR1 alleles, in parallel with linked multi-marker haplotype analysis of 13 highly polymorphic microsatellite markers located within 1 Mb of the FMR1 CGG repeat, and the AMELX/Y dimorphism for gender identification. The assay was optimised and validated on single lymphoblasts isolated from fragile X reference cell lines, and applied to a simulated PGD case and a clinical in vitro fertilisation (IVF)-PGD case. In the simulated PGD case, definitive diagnosis of the expected results was achieved for all ‘embryos’. In the clinical IVF-PGD case, delivery of a healthy baby girl was achieved after transfer of an expansion-negative blastocyst. FMR1 TP-PCR reliably detects presence of expansion mutations and obviates reliance on informative normal alleles for determining expansion status in female embryos. Together with multi-marker haplotyping and gender determination, misdiagnosis and diagnostic ambiguity due to allele dropout is minimised, and couple-specific assay customisation can be avoided.

scholarly journals A Decade of Molecular Preimplantation Genetic Diagnosis of 350 Blastomeres for Beta-Thalassemia Combined with HLA Typing, Aneuploidy Screening and Sex Selection in Iran

2021 ◽  
Author(s):  
Yeganeh Keshvar ◽  
Solmaz Sabeghi ◽  
Zohreh Sharifi ◽  
Kiyana Sadat Fatemi ◽  
Panti Fouladi ◽  
...  
Keyword(s):  
Preimplantation Genetic Diagnosis ◽  
Tandem Repeats ◽  
Genetic Diagnosis ◽  
Sex Selection ◽  
Hla Typing ◽  
Causative Mutation ◽  
Beta Thalassemia ◽  
Aneuploidy Screening ◽  
Cell Stage

Abstract Background: Preimplantation genetic diagnosis (PGD) has been developed to detect genetic disorders before pregnancy which is usually done on blastomeres biopsied from 8-cell stage embryos obtained from in vitro fertilization method (IVF).Here we report molecular PGD results for diagnosing of beta thalassemia (beta-thal) which are usually accompanied with evaluating chromosomal aneuploidies, HLA typing and sex selection.Methods: In this study, haplotype analysis was performed using short tandem repeats (STRs) in a multiplex nested PCR and the causative mutation was detected by Sanger sequencing.Results: We have performed PGDs on 350 blastomeres from 55 carrier couples; 142 blastomeres for beta-thal only, 75 for beta-thal and HLA typing, 76 for beta-thal in combination with sex selection, and 57 for beta-thal and aneuploidy screening. 150 blastomeres were transferable, 15 pregnancies were happened, and 11 babies born.We used 6 markers for beta-thal, 36 for aneuploidy screening, 32 for sex selection, and 35 for HLA typing. To our knowledge combining all these markers together and the number of STR markers are much more than any other studies which have ever done.Conclusions: PGD is a powerful diagnostic tool for carrier couples who desire to have a healthy child and wish to avoid medical abortion.

scholarly journals Preimplantation Genetic Diagnosis in Marfan Syndrome

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
N. F. Vlahos ◽  
O. Triantafyllidou ◽  
N. Vitoratos ◽  
C. Grigoriadis ◽  
G. Creatsas
Keyword(s):  
Marfan Syndrome ◽  
Preimplantation Genetic Diagnosis ◽  
Genetic Diagnosis ◽  
Male Infant ◽  
Caucasian Woman ◽  
Aortic Dilatation ◽  
Singleton Pregnancy ◽  
Vitro Fertilization ◽  
Transmission Of Disease

Marfan syndrome (MFS) is a systemic hereditable disorder of the connective tissue with mainly cardiovascular manifestations, such as aortic dilatation and dissection. We describe a case of a 32-year-old Caucasian woman, clinically asymptomatic with MFS who presented for genetic consultation to prevent the transmission of disease to her offspring. She underwent controlled ovarian stimulation (COH), in vitro fertilization (IVF) combined with preimplantation genetic diagnosis (PGD), and a singleton pregnancy with positive fetal heart rate was revealed. At 34 weeks’ gestation she delivered vaginally a healthy premature male infant weighting 2440 gr. The patient remained asymptomatic during pregnancy, delivery, and 3 months postpartum. It is has to be mentioned that the availability of PGD is essential to prevent the transmission of disease to the next generation.

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