scholarly journals Isolation of a nitrate-reducing bacteria strain from oil field brine and the inhibition of sulfate-reducing bacteria

2011 ◽  
Vol 10 (49) ◽  
pp. 10019-10029 ◽  
Author(s):  
Wen Zhuang ◽  
Jizhou Duan ◽  
Liye Chu ◽  
Hongbo Shao
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria

scholarly journals Oil Field Souring Control by Nitrate-Reducing Sulfurospirillum spp. That Outcompete Sulfate-Reducing Bacteria for Organic Electron Donors

2007 ◽  
Vol 73 (8) ◽  
pp. 2644-2652 ◽  
Author(s):  
Casey Hubert ◽  
Gerrit Voordouw
Keyword(s):  
Community Analysis ◽  
Packed Bed ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Sulfate Concentration ◽  
Key Factors ◽  
Sulfate Reducing ◽  
Production Of Hydrogen ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria

ABSTRACT Nitrate injection into oil reservoirs can prevent and remediate souring, the production of hydrogen sulfide by sulfate-reducing bacteria (SRB). Nitrate stimulates nitrate-reducing, sulfide-oxidizing bacteria (NR-SOB) and heterotrophic nitrate-reducing bacteria (hNRB) that compete with SRB for degradable oil organics. Up-flow, packed-bed bioreactors inoculated with water produced from an oil field and injected with lactate, sulfate, and nitrate served as sources for isolating several NRB, including Sulfurospirillum and Thauera spp. The former coupled reduction of nitrate to nitrite and ammonia with oxidation of either lactate (hNRB activity) or sulfide (NR-SOB activity). Souring control in a bioreactor receiving 12.5 mM lactate and 6, 2, 0.75, or 0.013 mM sulfate always required injection of 10 mM nitrate, irrespective of the sulfate concentration. Community analysis revealed that at all but the lowest sulfate concentration (0.013 mM), significant SRB were present. At 0.013 mM sulfate, direct hNRB-mediated oxidation of lactate by nitrate appeared to be the dominant mechanism. The absence of significant SRB indicated that sulfur cycling does not occur at such low sulfate concentrations. The metabolically versatile Sulfurospirillum spp. were dominant when nitrate was present in the bioreactor. Analysis of cocultures of Desulfovibrio sp. strain Lac3, Lac6, or Lac15 and Sulfurospirillum sp. strain KW indicated its hNRB activity and ability to produce inhibitory concentrations of nitrite to be key factors for it to successfully outcompete oil field SRB.

scholarly journals Two Marine Desulfotomaculum spp. of Different Origin are Capable of Utilizing Acetone and Higher Ketones

2021 ◽  
Author(s):  
Jasmin Frey ◽  
Sophie Kaßner ◽  
Bernhard Schink
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Thiamine Diphosphate ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria ◽  
Different Origin

AbstractDegradation of acetone and higher ketones has been described in detail for aerobic and nitrate-reducing bacteria. Among sulfate-reducing bacteria, degradation of acetone and other ketones is still an uncommon ability and has not been understood completely yet. In the present work, we show that Desulfotomaculum arcticum and Desulfotomaculum geothermicum are able to degrade acetone and butanone. Total proteomics of cell-free extracts of both organisms indicated an involvement of a thiamine diphosphate-dependent enzyme, a B12-dependent mutase, and a specific dehydrogenase during acetone degradation. Similar enzymes were recently described to be involved in acetone degradation by Desulfococcus biacutus. As there are so far only two described sulfate reducers able to degrade acetone, D. arcticum and D. geothermicum represent two further species with this capacity. All these bacteria appear to degrade acetone via the same set of enzymes and therefore via the same pathway.

scholarly journals Activation of short-chain ketones and isopropanol in sulfate-reducing bacteria

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jasmin Frey ◽  
Sophie Kaßner ◽  
Dieter Spiteller ◽  
Mario Mergelsberg ◽  
Matthias Boll ◽  
...  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Short Chain ◽  
Enzyme Assays ◽  
Protein Patterns ◽  
Sulfate Reducing ◽  
Protein Gel ◽  
Branched Chain ◽  
Reducing Bacteria ◽  
Coenzyme B ◽  
Nitrate Reducing Bacteria

Abstract Background Degradation of acetone by aerobic and nitrate-reducing bacteria can proceed via carboxylation to acetoacetate and subsequent thiolytic cleavage to two acetyl residues. A different strategy was identified in the sulfate-reducing bacterium Desulfococcus biacutus that involves formylation of acetone to 2-hydroxyisobutyryl-CoA. Results Utilization of short-chain ketones (acetone, butanone, 2-pentanone and 3-pentanone) and isopropanol by the sulfate reducer Desulfosarcina cetonica was investigated by differential proteome analyses and enzyme assays. Two-dimensional protein gel electrophoresis indicated that D. cetonica during growth with acetone expresses enzymes homologous to those described for Desulfococcus biacutus: a thiamine diphosphate (TDP)-requiring enzyme, two subunits of a B12-dependent mutase, and a NAD+-dependent dehydrogenase. Total proteomics of cell-free extracts confirmed these results and identified several additional ketone-inducible proteins. Acetone is activated, most likely mediated by the TDP-dependent enzyme, to a branched-chain CoA-ester, 2-hydroxyisobutyryl-CoA. This compound is linearized to 3-hydroxybutyryl-CoA by a coenzyme B12-dependent mutase followed by oxidation to acetoacetyl-CoA by a dehydrogenase. Proteomic analysis of isopropanol- and butanone-grown cells revealed the expression of a set of enzymes identical to that expressed during growth with acetone. Enzyme assays with cell-free extract of isopropanol- and butanone-grown cells support a B12-dependent isomerization. After growth with 2-pentanone or 3-pentanone, similar protein patterns were observed in cell-free extracts as those found after growth with acetone. Conclusions According to these results, butanone and isopropanol, as well as the two pentanone isomers, are degraded by the same enzymes that are used also in acetone degradation. Our results indicate that the degradation of several short-chain ketones appears to be initiated by TDP-dependent formylation in sulfate-reducing bacteria.

Characterization of the diversity of sulfate-reducing bacteria in soil and mining waste water environments by nucleic acid hybridization techniques

1994 ◽  
Vol 40 (11) ◽  
pp. 955-964 ◽  
Author(s):  
Anita J. Telang ◽  
Gerrit Voordouw ◽  
Sara Ebert ◽  
Neili Sifeldeen ◽  
Julia M. Foght ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Bacterial Communities ◽  
Genomic Dna ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Nucleic Acid Hybridization ◽  
Gene Probe ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Field Production

Nucleic acid hybridization techniques were used to characterize the sulfate-reducing bacterial communities at seven waste water and two soil sites in Canada. Genomic DNA was obtained from liquid enrichment cultures of samples taken from these nine sites. The liquid enrichment protocol favored growth of the sulfate-reducing bacterial component of the communities at these sites. The genomic DNA preparations were analyzed with (i) a specific gene probe aimed at a single genus (Desulfovibrio), (ii) a general 16S rRNA gene probe aimed at all genera of sulfate-reducing bacteria and other bacteria, and (iii) whole genome probes aimed at specific bacteria. This three-pronged approach provided information on the sulfate-reducing bacterial community structures for the nine sites. These were compared with each other and with the sulfate-reducing bacterial communities of western Canadian oil field production waters, studied previously. It was found that there is considerable diversity in the sulfate-reducing bacterial community at each site. Most sulfate-reducing bacteria isolated from distinct sites are genomically different and differ also from sulfate-reducing bacteria found in oil field production waters.Key words: sulfate-reducing bacteria, genomic diversity, nucleic acid hybridization, microbial community.

Characterization of sulfate-reducing bacteria isolated from oil-field waters

1996 ◽  
Vol 42 (3) ◽  
pp. 259-266 ◽  
Author(s):  
C. Tardy-Jacquenod ◽  
P. Caumette ◽  
R. Matheron ◽  
C. Lanau ◽  
O. Arnauld ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Oil Fields ◽  
Gulf Of Guinea ◽  
Sulfate Reducing ◽  
The North ◽  
The North Sea ◽  
Reducing Bacteria

The occurrence and metabolic capacities of sulfate-reducing bacteria (SRB) were studied in 23 water samples taken from producing wells at 14 different sites. Oil fields in France, the North Sea, and the Gulf of Guinea were selected and classified according to physicochemical parameters (salinity ranging from 0.3 to 120 g∙L−1 and temperature between 29 and 85 °C). After the distribution of SRB within oil fields was studied, several strains of SRB were isolated and characterized metabolically. Twenty of the thirty-seven strains were not related to any known species. Most of the identified strains were members of the genera Desulfovibrio and Desulfotomaculum by molecular, morphological, and physiological properties.Key words: sulfate-reducing bacteria, oil-field ecology, metabolic identification, biodiversity.

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