Influence of plant growth regulators on somatic embryogenesis induction from inner teguments of rubber (Hevea brasiliensis) seeds

The influence of stage of fruit development and plant growth regulators on somatic embryogenesis and the relation of cultivar response on somatic embryogenesis and subsequent plant development have been investigated in eight cultivars of pecan [Carya illinoensis (Wangenh.) C. Koch]. Explants from the micropylar region of the ovule were more embryogenic when removed from fruits in the liquid endosperm stage than were intact ovules from less-mature fruits or from cotyledonary segments of more-mature fruits. Explants conditioned on medium containing auxin alone or auxin + cytokinin produced more somatic embryos than medium containing cytokinin alone. Under the conditions of this study, frequency of embryogenesis, as well as the germination of somatic embryos leading to plant development, indicated appreciable variation among cultivars. Plant development was greatest by far from somatic embryos of `Schley' than other cultivars studied.

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Effect of plant growth regulators on indirect somatic embryogenesis of Kenaf (Hibiscus cannabinus)

Nigerian Journal of Genetics ◽

10.4314/njg.v20i1.42251 ◽

2008 ◽

Vol 20 (1) ◽

Author(s):

MO Balogun ◽

SR Akande ◽

BA Ogunbodede

Keyword(s):

Somatic Embryogenesis ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Hibiscus Cannabinus ◽

Indirect Somatic Embryogenesis

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Effects of plant growth regulators and other compounds on flow of latex in Hevea brasiliensis

Annals of Applied Biology ◽

10.1111/j.1744-7348.1968.tb03859.x ◽

1968 ◽

Vol 62 (1) ◽

pp. 159-173 ◽

Cited By ~ 6

Author(s):

P. D. ABRAHAM ◽

S. G. BOATMAN ◽

G. E. BLACKMAN ◽

R. G. POWELL

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Hevea Brasiliensis

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Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo (Solanum quitoense Lam.)

Agronomía Colombiana ◽

10.15446/agron.colomb.v32n2.43861 ◽

2014 ◽

Vol 32 (2) ◽

pp. 170-179 ◽

Cited By ~ 1

Author(s):

Hernando Criollo ◽

Margarita Perea ◽

Mariano Toribio ◽

Johanna Muñoz

Keyword(s):

Acetic Acid ◽

Somatic Embryogenesis ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Leaf Explants ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Plant Propagation ◽

Cotyledonary Leaf

Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 mM of NAA were added to the medium with sucrose (50.0 and 263.1 mM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 mM of NAA ; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 mM of NAA + 263.1 mM of sucrose (32%).

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Somatic embryogenesis in carrot without plant growth regulators.

Plant tissue culture letters ◽

10.5511/plantbiotechnology1984.6.92 ◽

1989 ◽

Vol 6 (2) ◽

pp. 92-94 ◽

Cited By ~ 1

Author(s):

Hajime FURUKAWA ◽

Chihiro MATSUBARA ◽

Norihiro SHIGEMATSU

Keyword(s):

Somatic Embryogenesis ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators

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Somatic Embryogenesis and Subsequent Plant Regeneration from Zygotic Embryo Derived Callus of Rubber (Hevea brasiliensis Muell. Arg).

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v27i1.35012 ◽

2017 ◽

Vol 27 (1) ◽

pp. 51-61 ◽

Cited By ~ 1

Author(s):

Md Mahbubur Rahman ◽

Maziah Mahmood ◽

Norhani Abdullah ◽

Noor Azmi Shaharuddin ◽

Waheeda Parvin

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Plant Growth ◽

Growth Regulators ◽

Hevea Brasiliensis ◽

Zygotic Embryo ◽

Rubber Tree ◽

Mass Propagation ◽

Half Strength ◽

First Time

A protocol has been developed for induction, maturation and germination of the zygotic embryo derived callus of the rubber tree (Hevea brasiliensis Muell. Arg.). The influence of plant growth regulators (PGRs) including 2,4‐D, α‐NAA, picloram, GA3 and TDZ on MS and MMS were studied. Optimum calli were induced on MS supplemented with 2.0 mg/l 2, 4‐D. The best callus growth and proliferation was recorded on MS fortified with 2.0 mg/l 2, 4‐D + 2.0 mg/l BAP + 0.5 mg/l NAA. The maximum embryonic calli were induced on MS + 2.0 mg/l 2, 4‐D + 2.0 mg/l Kn medium. Embryo induction, differentiation and maturation were obtained on MMS (MS +Vit B5). The rooted plantlets were produced on half strength MS without any supplements.The novelty of this study is the induction of embryos and plant regeneration from zygotic embryo explants of Hevea for the first time. The protocol developed in this study will facilitate mass propagation of high yielding rubber clones as well as to develop transgenic rubber plants with desired genes through genetic transformation.Plant Tissue Cult. & Biotech. 27(1): 51-61, 2017 (June)

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