In vitro regeneration of disease free enset [Ensete ventricosum (Welw) Cheesman] planting materials from bacterial wilt diseased plants using shoot tip culture

In the Western highlands of Cameroon where sweetpotato (Ipomoea batatas L.) is an inevitable staple crop during the dry season, farmers cherish local which are presently under the risk of extinction due to accumulation of pest and diseases. Guaranteeing sustainable Sweetpotato production in this region therefore requires providing farmers with disease free planting materials such as tissue cultures. This study was conducted to investigate the feasibility of introducing disease free planting materials from meristem culture of EX-KOLB, EX-GUZANG2, EX-WIDIKUM3 and EX-NKAMBE1 which are four regional sweetpotato varieties common in the Western highlands of Cameroon. Test tubes containing growing media and meristems from each of the varieties were labeled and laid out in a completely randomized experimental design (CRD) with four replications and four treatments. Data on the number of sprouted meristems, number of rooted meristems, number of meristems contaminated, number of meristems that died, average heights of developed plants, average number of nodes on the developed plants, and average number of leaves on the developed plants was collected over a period of 8 weeks at 2 weeks interval. Meristems from all the landraces sprouted in the media used and the differences in the mean number of sprouted meristem between the landraces were significant (P<0.05). EX-GUZANG2 gave the highest mean of sprouted meristems of (2.75) and EX-WIDIKUM3 the lowest (0.75). According to ANOVA response of meristems from the four landraces to the media used differed significantly only in number sprouted, number of contaminated and number dead meristems as well as number of nodes(P<0.05). No significant differences were observed with number of contaminated and dead meristems, rooting as well plant height, number of leaves and nodes formed(P>0.05).This genotype-dependent response to media used for in vitro regeneration by plants has been observed by many studies. However meristems from EX-GUZANG2 and EX-KOLB showed greater positive respond to this media and merit further studies for the purpose of this experiment.

Download Full-text

Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽

10.3390/agronomy11020320 ◽

2021 ◽

Vol 11 (2) ◽

pp. 320

Author(s):

Nisar Ahmad Zahid ◽

Hawa Z.E. Jaafar ◽

Mansor Hakiman

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Zingiber Officinale ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Zingiber Officinale Roscoe ◽

Planting Materials ◽

Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

Download Full-text

Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture

Genetics and Molecular Research ◽

10.4238/gmr16029556 ◽

2017 ◽

Vol 16 (2) ◽

Cited By ~ 3

Author(s):

M.J.S. Carvalho ◽

E.J. Oliveira ◽

A.S. Souza ◽

J.S. Pereira ◽

M.S.A.S. Diamantino ◽

...

Keyword(s):

Shoot Tip ◽

Shoot Tip Culture

Download Full-text

CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

Revista Árvore ◽

10.1590/0100-67622015000300004 ◽

2015 ◽

Vol 39 (3) ◽

pp. 439-445 ◽

Cited By ~ 4

Author(s):

Laureen Michelle Houllou ◽

Robson Antônio de Souza ◽

Elizabete Cristina Pacheco dos Santos ◽

José Jackson Pereira da Silva ◽

Marta Ribeiro Barbosa ◽

...

Keyword(s):

Azadirachta Indica ◽

Callus Formation ◽

In Vitro Regeneration ◽

Regenerative Process ◽

Shoot Tips ◽

Shoot Tip ◽

Stem Base ◽

Different Characteristics

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.

Download Full-text