scholarly journals In vitro plantlet regeneration and genetic transformation of sponge gourd (Luffa cylindrica L.)

2013 ◽  
Vol 7 (6) ◽  
pp. 244-252 ◽  
Author(s):  
Sujatha D.
Keyword(s):  
Genetic Transformation ◽  
Plantlet Regeneration ◽  
Luffa Cylindrica ◽  
Sponge Gourd

In vitro evaluation of fungicide and bioagents against Pythium aphanidermatum

2016 ◽  
Vol 1 (02) ◽  
pp. 213-217
Author(s):  
Abhishek Mishra ◽  
Ved Ratan ◽  
Gautam Veer Chauhan ◽  
Akash Tomar
Keyword(s):  
Root Rot ◽  
Soft Rot ◽  
Pythium Aphanidermatum ◽  
Fruit Rot ◽  
Luffa Cylindrica ◽  
Cercospora Leaf Spot ◽  
Trichoderma Koningii ◽  
Sponge Gourd ◽  
Considerable Loss

The sponge gourd (Luffa cylindrica Roem) is one of the most popular vegetable. There are a number of diseases like Powdery mildew, Downey mildew, Anthracnose, Fusarium root rot, Cercospora leaf spot, Soft rot and cottony fruit rot, they cause considerable loss to sponge gourd in term of quality and quantity both. During the survey in the year 2015-16, it was found that among all the diseases cottony fruit rot caused by Pythium aphanidermatum was a very destructive disease of this important crop. To manage the loss of yield due to this disease twelve fungicide and eight bioagents were evaluate against the pathogen (in-vitro) in biocontrol lab, Deptt. of plant pathology during 2015-16. Among all the tried fungicide, Master, Propioconazole and Tubuconazole were most effective for inhibition of radial growth of Pythium aphanidermatum and it was recorded no growth. In case of bioagents Trichoderma koningii and Trichoderma harzianum wear most effective for inhibition of test pathogen and it was 15.33mm and 16.66mm respectively.

scholarly journals Comparison of the effects of growth regulators on in vitro regeneration of ridge gourd and sponge gourd through shoot

1970 ◽  
Vol 18 ◽  
pp. 88-93
Author(s):  
E Nahar ◽  
ME Haque ◽  
B Sikdar
Keyword(s):  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Luffa Cylindrica ◽  
Sponge Gourd ◽  
Seed Propagation ◽  
Luffa Acutangula

Context: Luffa cylindrica and Luffa acutangula are highly cross pollinated crops and propagated mainly by seeds. Genetic stability cannot be maintained easily by seed propagation. It can be maintain by developing special vegetative technique through tissue culture.   Objectives: To compare the effects of growth regulators between two species of Luffa using shoot tips for develop the rapid, simple and efficient in vitro regeneration protocol.   Materials and Methods: Shoot tips used were collected from in vivo grown plants. They were excised from plants and surface sterilized by HgCl2 treatment. The isolated tips were cultured on semisolid MS medium supplemented with different concentrations and combinations of different growth regulators.   Results: The highest result of direct shoot multiplication of ridge gourd was observed using 2 mg/l BAP + 0.2 mg/l GA and in case of sponge gourd it was 1.5 mg/l BAP. For callus induction significant result was found using 4 mg/l BAP + 0.2 mg/l NAA in ridge gourd and 3 mg/l BAP + 0.2 mg/l NAA in sponge gourd. Indirect regeneration was performed by subculturing organogenic callus of sponge gourd on MS with 1.5 mg/l BAP + 0.2 mg/l GA3 and the callus of ridged gourd on MS + 1.5 mg/l BAP + 0.2 mg/l NAA + 0.2 mg/l nicotinic acid. Regenerated shoots of both species were rooted well on MS containing NAA at low concentration.   Conclusion: Hormonal differences and simple rapid in vitro regeneration protocol of L. cylindrica and L. acutangula have been established.   Keywords: Growth regulators; in vitro regeneration; ridge gourd; sponge gourd. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8781 JBS 2010; 18(0): 88-93

In vitro plantlet regeneration and Agrobacterium tumefaciens-mediated genetic transformation of Indian Kino tree (Pterocarpus marsupium Roxb.)

2013 ◽  
Vol 35 (12) ◽  
pp. 3437-3446 ◽  
Author(s):  
Radhika Tippani ◽  
Rajesh Yarra ◽  
Mallesham Bulle ◽  
Mahendar Porika ◽  
Sadanandam Abbagani ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Plantlet Regeneration ◽  
Pterocarpus Marsupium

scholarly journals IN VITRO SHOOT REGENERATION OF RUBUS PLANTS USING PETIOLES AND INTERNODES.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1137d-1137
Author(s):  
Mahmoud A. Hassan ◽  
Harry Jan Swartz
Keyword(s):  
Genetic Transformation ◽  
Shoot Regeneration ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
In Vitro Shoot Regeneration

An efficient protocol for plantlet regeneration using petioles and internodes of two blackberry cultivars has been developed for use in genetic transformation. Maximum shoot regeneration was induced on MS medium supplemented with 5 μM Thidiazuron (TDZ) and 0.5 μM IBA. Preconditioning the source shoots on 0.5 μM TDZ containing MS medium did increase the number of regenerated shoots/explant, but did not affect the regeneration percentage. The antibiotic, kanamycin, significantly reduced the growth and regeneration of explants at 10 mg/L or higher. In contrast, cefatoxime at 100-500 mg/L increased explant growth and percentage regeneration.

scholarly journals Development of fast and high-efficiency sponge-gourd fibers (Luffa cylindrica)/hydroxyapatite composites for removal of lead and methylene blue

2021 ◽  
pp. 103281
Author(s):  
Ahmed A. Oun ◽  
Kholod H. Kamal ◽  
Khaled Farroh ◽  
Esmat F. Ali ◽  
Mohamed A. Hassan
Keyword(s):  
Methylene Blue ◽  
High Efficiency ◽  
Luffa Cylindrica ◽  
Sponge Gourd

scholarly journals In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

2014 ◽  
Vol 8 (24) ◽  
pp. 847-856
Author(s):  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
Gopal Ghanta Rama ◽  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
...  
Keyword(s):  
Callus Induction ◽  
Plantlet Regeneration

In vitro plantlet regeneration of Olea europaea ssp. maderensis

2003 ◽  
Vol 97 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Conceição V. Santos ◽  
Gina Brito ◽  
Gloria Pinto ◽  
Henrique M.A.C. Fonseca
Keyword(s):  
Olea Europaea ◽  
Plantlet Regeneration ◽  
Olea Europaéa

Effects of chitosan coating on postharvest quality and shelf life of sponge gourd (Luffa cylindrica) during storage

2014 ◽  
Vol 166 ◽  
pp. 1-8 ◽  
Author(s):  
Cong Han ◽  
Jinhua Zuo ◽  
Qing Wang ◽  
Lijing Xu ◽  
Baiqiang Zhai ◽  
...  
Keyword(s):  
Shelf Life ◽  
Postharvest Quality ◽  
Luffa Cylindrica ◽  
Chitosan Coating ◽  
Sponge Gourd ◽  
Quality And Shelf Life

scholarly journals In vitro Regeneration of Dalbergia sissoo Roxb. and the Potential for Genetic Transformation

2012 ◽  
Vol 40 (2) ◽  
pp. 140 ◽  
Author(s):  
Hafiz Mamoon REHMAN ◽  
Iqrar Ahmad RANA ◽  
Siddra IJAZ ◽  
Ghulam MUSTAFA ◽  
Faiz Ahmad JOYIA ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Genetic Transformation ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Induction Medium ◽  
Native Forest ◽  
Ms Medium ◽  
Dalbergia Sissoo ◽  
Callus Induction Medium

Dalbergia sissoo Roxb. ex DC. (Sissoo) is a native forest tree species in Pakistan. Many ecological and economical uses are associated with this premier timber species, but dieback disease is of major concern. The objective of this study was to develop a protocol for in vitro regeneration of Sissoo that could serve as target material for genetic transformation, in order to improve this species. Callus formation and plantlet regeneration was achieved by culturing cotyledons, immature seeds, and mature embryos on a modified Murashige and Skoog (1962) (MS) medium supplemented with plant growth regulators. Callus induction medium containing 2.71 ?M 2, 4-dichlorophenoxyacetic acid (2,4-D) and 0.93 ?M kinetin produced better callus on all explants tested compared to other treatments, such as 8.88 ?M 6-benzylaminopurine (BA) and 2.69 ?M ?-naphthalene acetic acid (NAA), or 2.71 ?M 2, 4-D and 2.69 ?M NAA. Shoot regeneration was best on MS medium containing 1.4 ?M NAA and 8.88 ?M BA compared to other treatments, such as 1.4 ?M NAA and 9.9 ?M kinetin, or 2.86 ?M indole-3-acetic acid and 8.88 ?M BA. Murashige and Skoog medium containing 1.4 NAA ?M and 8.88 ?M BA was better in general for regeneration regardless of callus induction medium and the type of explant used. Rooting was best on half-strength MS medium with 7.35 ?M indole-3-butyric acid. Regenerated plantlets were acclimatized for plantation in the field. Preliminary genetic transformation potential of D. sissoo was evaluated by particle bombardment of callus explants with a pUbiGus vector. The bombarded tissue showed transient Gus activity 1week after bombardment. Transformation of this woody tree is possible provided excellent regeneration protocols. The best combination for regeneration explained in this study is one of such protocols.

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