scholarly journals Inheritance and detection of QTL in cowpea resistance to brown blotch disease

2021 ◽  
Vol 13 (3) ◽  
pp. 123-135
Author(s):  
G. THIO Ibié ◽  
Baptiste TIGNEGRE Jean ◽  
DRABO Inoussa ◽  
T. B. BATIENO Joseph ◽  
P. ZIDA Elisabeth ◽  
...  
Keyword(s):  
Cowpea Resistance ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Confirmation de QTL et validation de marqueurs SNPs associés à la résistance du niébé à Colletotrichum capsici, agent responsable de la maladie des taches brunes

2021 ◽  
Vol 15 (3) ◽  
pp. 909-922
Author(s):  
Ibié G. Thio ◽  
Nofou Ouedraogo ◽  
Serge W.F.M. Zida ◽  
Joseph T.B. Batieno ◽  
Elisabeth P. Zida ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Burkina Faso ◽  
Vigna Unguiculata ◽  
Grain Legume ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Colletotrichum Capsici ◽  
Cowpea Resistance ◽  
Brown Blotch ◽  
Brown Blotch Disease

Le niébé (Vigna unguiculata (L.) Walp.) est une légumineuse à graine très importante et constitue la principale source de protéines végétales pour l’alimentation des populations d’Afrique Subsaharienne. Sa production au Burkina Faso est entravée par la maladie des taches brunes provoquée par un champignon, Colletotrichum capsici (Syd.) Butler et Bisby. C’est dans la perspective d’accroître la productivité du niébé que nous avons entrepris de renforcer la lutte variétale contre cet agent pathogène. L’identification de marqueurs SNPs (Single Nucleotide Polymorphism) et QTL liés à la résistance à la maladie des taches brunes a été entrepris à partir d’une population biparentale F2 issus du croisement entre la variété sensible Tiligré et celle résistante KN-1. L’analyse QTL de la résistance du niébé à C. capsici à partir de la méthode ICIM add. a permis de confirmer et de valider respectivement un QTL majeur dénommé qBBDR2.1 et 9 marqueurs SNPs convertis, lesquels ont été cartographiés sur le chromosome Vu02 du niébé. Ce QTL dominant a présenté des effets additifs élevés liés aux allèles favorables de KN-1 et des valeurs de PVE de l’ordre de 51,50% et 55,33%, respectivement aux 21ème et 28ème JAI. English title: Confirmation of QTL mapping and validation of SNPs markers associated to cowpea resistance to Colletotrichum capsici, causal agent of brown blotch disease Cowpea (Vigna unguiculata (L.)Walp.) is one of the most important grain legume crops and constitutes the main source of plant protein for people food in sub-Saharan Africa. Cowpea production in Burkina Faso is constrained by brown blotch disease caused by a fungal,  Colletotrichum capsici (Syd.) Butler and Bisby. In order to increase cowpea productivity we initiated a project to enhance host plant resistance to control the pathogen. The identification of SNP (Single Nucleotide Polymorphism) markers and QTL associated with brown blotch disease resistance was undertaken from a bi-parental F2 population resulting from a cross between the sensitive variety Tiligre and the resistant KN-1 to the disease. QTL analysis of cowpea resistance to C. capsici using the ICIM add method. Allowed to confirm and validate respectively a major QTL named qBBDR2.1 and 9 converted SNP markers, which were mapped on cowpea chromosome Vu02. This dominant QTL showed higher additive effects associated to alleles from KN-1 and PVE values of 51.50% and 55.33% respectively at 21 and 28 days after inoculation

Characterization of bacteriophage ϕPto-bp6g, a novel phage that lyses Pseudomonas tolaasii causing brown blotch disease in mushrooms

2012 ◽  
Vol 91 (3) ◽  
pp. 514-519 ◽  
Author(s):  
Hanh Thi Dieu Nguyen ◽  
Soojin Yoon ◽  
Min-Hee Kim ◽  
Young-Kee Kim ◽  
Moon-Young Yoon ◽  
...  
Keyword(s):  
Pseudomonas Tolaasii ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Biochemical and physiological aspects of brown blotch disease ofAgaricus bisporus

1999 ◽  
Vol 23 (5) ◽  
pp. 591-614 ◽  
Author(s):  
C. Soler-Rivas ◽  
S. Jolivet ◽  
N. Arpin ◽  
J.M. Olivier ◽  
H.J. Wichers
Keyword(s):  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Resistance Sources to Brown Blotch Disease (Pseudomonas tolaasii) in a Diverse Collection of Pleurotus Mushroom Strains

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 227 ◽  
Author(s):  
Benjamin Azu Okorley ◽  
Frederick Leo Sossah ◽  
Dan Dai ◽  
Shuai Xu ◽  
Zhenghui Liu ◽  
...  
Keyword(s):  
Pseudomonas Tolaasii ◽  
Resistance Sources ◽  
Controlled Conditions ◽  
Resistant Strains ◽  
Brown Blotch ◽  
Brown Blotch Disease ◽  
Moderately Resistant

Brown blotch disease (BBD) caused by Pseudomonas tolaasii is one of the most devastating diseases of Pleurotus spp. worldwide. Breeding for resistant strains is the most effective method for controlling BBD. To identify resistant germplasm for BBD management, 97 strains comprising 21 P. cf. floridanus, 20 P. ostreatus, and 56 P. pulmonarius were screened by two different methods; namely, inoculation of the pathogen on the mushroom pileus (IMP) and on the spawned substrate (IMSS) under controlled conditions. Out of the 97 strains screened, 22 P. pulmonarius, and four P. cf. floridanus were moderately resistant to BBD using the IMP method. Eleven P. pulmonarius, six P. cf. florida, and one P. ostreatus strains were highly resistant to BBD using the IMSS method. All of the 97 strains showed varying degrees of susceptibility using the IMP method, but eight strains were completely resistant using the IMSS method. Combining these two methods, five strains were highly resistant (four P. pulmonarius and one P. cf. floridanus) and 11 were moderately resistant (eight P. pulmonarius and three P. cf. floridanus). The resistance sources to P. tolaasii identified in P. pulmonarius and P. cf. floridanus could be used for further breeding of Pleurotus spp.

scholarly journals Visible-Near Infrared Hyperspectral Imaging for the Identification and Discrimination of Brown Blotch Disease on Mushroom (Agaricus Bisporus) Caps

2010 ◽  
Vol 18 (5) ◽  
pp. 341-353 ◽  
Author(s):  
Edurne Gaston ◽  
Jesús M. Frías ◽  
Patrick J. Cullen ◽  
Colm P. O'Donnell ◽  
Aoife A. Gowen
Keyword(s):  
Hyperspectral Imaging ◽  
Agaricus Bisporus ◽  
Near Infrared ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Application of Siderotyping for Characterization of Pseudomonas tolaasii and “Pseudomonas reactans” Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms

2000 ◽  
Vol 66 (11) ◽  
pp. 4834-4841 ◽  
Author(s):  
Patricia Munsch ◽  
Valerie A. Geoffroy ◽  
Tapani Alatossava ◽  
Jean-Marie Meyer
Keyword(s):  
Isoelectric Focusing ◽  
Agaricus Bisporus ◽  
White Line ◽  
Disease Symptoms ◽  
Line Test ◽  
Brown Blotch ◽  
Uptake Studies ◽  
Brown Blotch Disease ◽  
Reference Strains

ABSTRACT Pyoverdine isoelectric focusing analysis and pyoverdine-mediated iron uptake were used as siderotyping methods to analyze a collection of 57 northern and central European isolates of P. tolaasiiand “P. reactans.” The bacteria, isolated from cultivated Agaricus bisporus or Pleurotus ostreatus mushroom sporophores presenting brown blotch disease symptoms, were identified according to the white line test (W. C. Wong and T. F. Preece, J. Appl. Bacteriol. 47:401–407, 1979) and their pathogenicity towards A. bisporus and were grouped into siderovars according to the type of pyoverdine they produced. Seventeen P. tolaasii isolates were recognized, which divided into two siderovars, with the first one containing reference strains and isolates of various geographical origins while the second one contained Finnish isolates exclusively. The 40 “P. reactans” isolates divided into eight siderovars. Pyoverdine isoelectric focusing profiles and cross-uptake studies demonstrated an identity for some “P. reactans” isolates, with reference strains belonging to theP. fluorescens biovars II, III, or V. Thus, the easy and rapid methods of siderotyping proved to be reliable by supporting and strengthening previous taxonomical data. Moreover, two potentially novel pyoverdines characterizing one P. tolaasiisiderovar and one “P. reactans” siderovar were found.

scholarly journals Helper bacteria halt and disarm mushroom pathogens by linearizing structurally diverse cyclolipopeptides

2020 ◽  
Vol 117 (38) ◽  
pp. 23802-23806
Author(s):  
Ron Hermenau ◽  
Susann Kugel ◽  
Anna J. Komor ◽  
Christian Hertweck
Keyword(s):  
Lactone Ring ◽  
Imaging Mass Spectrometry ◽  
Bacterial Pathogen ◽  
Biosynthetic Gene Cluster ◽  
Detoxification Mechanism ◽  
Brown Blotch ◽  
Button Mushrooms ◽  
Brown Blotch Disease ◽  
White Button Mushrooms

The bacterial pathogenPseudomonas tolaasiiseverely damages white button mushrooms by secretion of the pore-forming toxin tolaasin, the main virulence factor of brown blotch disease. Yet, fungus-associated helper bacteria of the genusMycetocola(Mycetocola tolaasinivoransandMycetocola lacteus) may protect their host by an unknown detoxification mechanism. By a combination of metabolic profiling, imaging mass spectrometry, structure elucidation, and bioassays, we found that the helper bacteria inactivate tolaasin by linearizing the lipocyclopeptide. Furthermore, we found thatMycetocolaspp. impair the dissemination of the pathogen by cleavage of the lactone ring of pseudodesmin. The role of pseudodesmin as a major swarming factor was corroborated by identification and inactivation of the corresponding biosynthetic gene cluster. Activity-guided fractionation of theMycetocolaproteome, matrix-assisted laser desorption/ionization (MALDI) analyses, and heterologous enzyme production identified the lactonase responsible for toxin cleavage. We revealed an antivirulence strategy in the context of a tripartite interaction that has high ecological and agricultural relevance.

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