scholarly journals Application of Siderotyping for Characterization of Pseudomonas tolaasii and “Pseudomonas reactans” Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms

2000 ◽  
Vol 66 (11) ◽  
pp. 4834-4841 ◽  
Author(s):  
Patricia Munsch ◽  
Valerie A. Geoffroy ◽  
Tapani Alatossava ◽  
Jean-Marie Meyer
Keyword(s):  
Isoelectric Focusing ◽  
Agaricus Bisporus ◽  
White Line ◽  
Disease Symptoms ◽  
Line Test ◽  
Brown Blotch ◽  
Uptake Studies ◽  
Brown Blotch Disease ◽  
Reference Strains

ABSTRACT Pyoverdine isoelectric focusing analysis and pyoverdine-mediated iron uptake were used as siderotyping methods to analyze a collection of 57 northern and central European isolates of P. tolaasiiand “P. reactans.” The bacteria, isolated from cultivated Agaricus bisporus or Pleurotus ostreatus mushroom sporophores presenting brown blotch disease symptoms, were identified according to the white line test (W. C. Wong and T. F. Preece, J. Appl. Bacteriol. 47:401–407, 1979) and their pathogenicity towards A. bisporus and were grouped into siderovars according to the type of pyoverdine they produced. Seventeen P. tolaasii isolates were recognized, which divided into two siderovars, with the first one containing reference strains and isolates of various geographical origins while the second one contained Finnish isolates exclusively. The 40 “P. reactans” isolates divided into eight siderovars. Pyoverdine isoelectric focusing profiles and cross-uptake studies demonstrated an identity for some “P. reactans” isolates, with reference strains belonging to theP. fluorescens biovars II, III, or V. Thus, the easy and rapid methods of siderotyping proved to be reliable by supporting and strengthening previous taxonomical data. Moreover, two potentially novel pyoverdines characterizing one P. tolaasiisiderovar and one “P. reactans” siderovar were found.

Characterization of bacteriophage ϕPto-bp6g, a novel phage that lyses Pseudomonas tolaasii causing brown blotch disease in mushrooms

2012 ◽  
Vol 91 (3) ◽  
pp. 514-519 ◽  
Author(s):  
Hanh Thi Dieu Nguyen ◽  
Soojin Yoon ◽  
Min-Hee Kim ◽  
Young-Kee Kim ◽  
Moon-Young Yoon ◽  
...  
Keyword(s):  
Pseudomonas Tolaasii ◽  
Brown Blotch ◽  
Brown Blotch Disease

scholarly journals Molecular characterization of stolbur phytoplasmas in pepper and tomato from Bulgaria

2020 ◽  
Vol 18 ◽  
pp. 00025
Author(s):  
Dimitriyka Sakalieva
Keyword(s):  
Molecular Characterization ◽  
Reference Strain ◽  
Rflp Analysis ◽  
Plant Diseases ◽  
Vegetable Crops ◽  
Disease Symptoms ◽  
Natural Plant ◽  
Conserved Genes ◽  
Reference Strains

Tomato and pepper are the main vegetable crops cultivated in Bulgaria. Phytoplasma diseases, mainly stolbur, are important plant diseases for these crops in Bulgaria. The goal of the present paper was to verify association of phytoplasmas with the observed disease symptoms in tomato and pepper and to identify the phytoplasmas detected using RFLP analysis of conserved genes and other uncharacterised phytoplasma chromosomal regions. The presence of phytoplasmas was confirmed in all the samples of tomato and pepper showing typical stolbur symptoms. A phytoplasm sample, which caused severe symptoms, showed the same pattern as the reference strain Mol, while all other phytoplasmic reference strains showed different polymorphisms. RFLP profiles were found useful in distinguishing phytoplasmas in stolbur subgroup (16SrXII-A) in natural plant hosts.

scholarly journals Visible-Near Infrared Hyperspectral Imaging for the Identification and Discrimination of Brown Blotch Disease on Mushroom (Agaricus Bisporus) Caps

2010 ◽  
Vol 18 (5) ◽  
pp. 341-353 ◽  
Author(s):  
Edurne Gaston ◽  
Jesús M. Frías ◽  
Patrick J. Cullen ◽  
Colm P. O'Donnell ◽  
Aoife A. Gowen
Keyword(s):  
Hyperspectral Imaging ◽  
Agaricus Bisporus ◽  
Near Infrared ◽  
Brown Blotch ◽  
Brown Blotch Disease

Phenotypic traits of fluorescent pseudomonads causing bacterial blotch of Agaricus bisporus mushrooms and other mushroom-derived fluorescent pseudomonads

1984 ◽  
Vol 30 (3) ◽  
pp. 360-367 ◽  
Author(s):  
P. A. Zarkower ◽  
P. J. Wuest ◽  
D. J. Royse ◽  
B. Myers
Keyword(s):  
Agaricus Bisporus ◽  
Soft Rot ◽  
Fluorescent Pseudomonads ◽  
Phenotypic Traits ◽  
Potato Tissue ◽  
White Line ◽  
Reliable Assessment ◽  
Unique Identity ◽  
Line Test ◽  
Dissociative Tendencies

Fluorescent pseudomonads isolated from diseased Agaricus bisporus mushrooms (bacterial blotch), internal mushroom stipe tissue, and reference cultures were subjected to an array of determinative tests including a recently described white-line reaction. All isolates recovered from fresh, healthy mushroom tissue proved to be in the range of Pseudomonas putida or P. fluorescens types, producing oxidase and arginine dehydrolase with growth at 4 °C, while failing to cause soft rot of potato tissue or to blotch mushroom tissue, produce pigment on King's medium A, or to grow at 41 °C. Significantly, 68 cultures identified as P. fluorescens belonged to biotype G, 35 having a similar nutritional profile, which suggests a unique identity. Six of eight P. tolaasii reference cultures were pathogens but their placement into a defined biotype is speculative. Dissociative tendencies were commonplace with all isolates. The white-line test was employed with P. tolaasii reference cultures. Those which caused blotch had an R reaction, white-line test. Of 90 isolates from diseased tissue, 76 were indicators (I) and 14 nonreactors (NR). The white-line reaction occurred in King's medium B as described and on three additional substrates. Hundreds of isolates responded as expected with either an I or an R reaction even though stored for up to 2 years. Reliable assessment of P. tolaasii pathogenicity using excised pileus tissue required three replicates read following 24 h incubation.

scholarly journals Functional characterization of the adenine transporter EaAdeP from the fire blight pathogen Erwinia amylovora and its effect on disease establishment in apples and pears

2020 ◽  
Vol 367 (20) ◽  
Author(s):  
Candace R Alexander ◽  
Regan B Huntley ◽  
Neil P Schultes ◽  
George S Mourad
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora ◽  
Functional Characterization ◽  
Biochemical Properties ◽  
Apple Fruit ◽  
Infection Process ◽  
Disease Symptoms ◽  
Uptake Studies ◽  
Important Disease

ABSTRACT Erwinia amylovora is the causal agent of fire blight, an economically important disease of apples and pears. As part of the infection process, Er. amylovora propagates on different plant tissues each with distinct nutrient environments. Here, the biochemical properties of the Er. amylovora adenine permease (EaAdeP) are investigated. Heterologous expression of EaAdeP in nucleobase transporter-deficient Escherichia coli strains, coupled with radiolabel uptake studies, revealed that EaAdeP is a high affinity adenine transporter with a Km of 0.43 ± 0.09 μM. Both Es. coli and Er. amylovora carrying extra copies of EaAdeP are sensitive to growth on the toxic analog 8-azaadenine. EaAdeP is expressed during immature pear fruit infection. Immature pear and apple fruit virulence assays reveal that an E. amylovora ΔadeP::Camr mutant is still able to cause disease symptoms, however, with growth at a lower level, indicating that external adenine is utilized in disease establishment.

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