Late blight resistance status in wild potato species against Indian population of Phytophthora infestans

2018 ◽  
Vol 75 (1) ◽  
pp. 99 ◽  
Author(s):  
Vinay Bhardwaj ◽  
Dalamu ◽  
A.K. Srivastava ◽  
Sanjeev Sharma ◽  
Vinod Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Indian Population ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species

scholarly journals Detection of Novel QTLs for Late Blight Resistance Derived from the Wild Potato Species Solanum microdontum and Solanum pampasense

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 732
Author(s):  
Fergus Meade ◽  
Ronald Hutten ◽  
Silke Wagener ◽  
Vanessa Prigge ◽  
Emmet Dalton ◽  
...  
Keyword(s):  
Late Blight ◽  
Resistance Genes ◽  
Late Blight Resistance ◽  
Gene Clusters ◽  
Genetic Maps ◽  
Blight Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species ◽  
Solanum Microdontum

Wild potato species continue to be a rich source of genes for resistance to late blight in potato breeding. Whilst many dominant resistance genes from such sources have been characterised and used in breeding, quantitative resistance also offers potential for breeding when the loci underlying the resistance can be identified and tagged using molecular markers. In this study, F1 populations were created from crosses between blight susceptible parents and lines exhibiting strong partial resistance to late blight derived from the South American wild species Solanum microdontum and Solanum pampasense. Both populations exhibited continuous variation for resistance to late blight over multiple field-testing seasons. High density genetic maps were created using single nucleotide polymorphism (SNP) markers, enabling mapping of quantitative trait loci (QTLs) for late blight resistance that were consistently expressed over multiple years in both populations. In the population created with the S. microdontum source, QTLs for resistance consistently expressed over three years and explaining a large portion (21–47%) of the phenotypic variation were found on chromosomes 5 and 6, and a further resistance QTL on chromosome 10, apparently related to foliar development, was discovered in 2016 only. In the population created with the S. pampasense source, QTLs for resistance were found in over two years on chromosomes 11 and 12. For all loci detected consistently across years, the QTLs span known R gene clusters and so they likely represent novel late blight resistance genes. Simple genetic models following the effect of the presence or absence of SNPs associated with consistently effective loci in both populations demonstrated that marker assisted selection (MAS) strategies to introgress and pyramid these loci have potential in resistance breeding strategies.

Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana)

2005 ◽  
Vol 111 (6) ◽  
pp. 1201-1214 ◽  
Author(s):  
F.G. Villamon ◽  
D.M. Spooner ◽  
M. Orrillo ◽  
E. Mihovilovich ◽  
W. Pérez ◽  
...  
Keyword(s):  
Late Blight ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species

scholarly journals LATE BLIGHT RESISTANCE OF WILD POTATO SPECIES UNDER FIELD CONDITIONS IN THE NORTHWEST OF RUSSIA

2020 ◽  
Vol 180 (4) ◽  
pp. 159-169 ◽  
Author(s):  
N. M. Zoteyeva
Keyword(s):  
Late Blight ◽  
Natural Infection ◽  
Late Blight Resistance ◽  
Field Resistance ◽  
Wild Potato ◽  
Wild Potato Species ◽  
Potato Species ◽  
Wide Range ◽  
Foliar Resistance ◽  
High Level

Background. Despite the great efforts made by breeders, late blight remains a paramount cause of significant potato harvest losses. Introgression of various resistance genes from wild Solanum L. species is the main method to increase the resistance in potato cultivars. Field resistance is considered to be more durable than those induced by the action of single R genes. To this end, resistance sources should be selected from а wide range of species under severe natural infection.Material and methods. As the material for evaluation, 1141 accessions of 99 wild potato species belonging to 15 taxonomic series according to the system of J. Hawkes were used. Each accession was assessed for 3–5 years. A 1–9 point scale was employed to score the damage of plants every week starting from the first symptoms of the disease, where 9 meant the absence of any symptoms, and 1 the entirely damaged plant. The plants scoring 6 to 9 points were considered resistant.Results and conclusions. As a result of the long-term field observations, wild potato species, represented in the current evaluation by numerous accessions, were characterized for foliar resistance to late blight; individual introductions resistant to late blight were also identified. Some of those studied in the 1980s showed high resistance in the end of the 1990s through the 2000s. The highest percentage of resistant accessions/species was identified among the species with areas of distribution in Mexico. A group of Central American species and large part of species with areas of distribution in South America expressed high level of interspecific polymorphism in foliar resistance to late blight.

scholarly journals Virulence of Phytophthora infestans isolates from potato in Spain

2021 ◽  
Vol 57 (No. 4) ◽  
pp. 279-288
Author(s):  
Jose Ignacio Ruiz de Galarreta ◽  
Alba Alvarez-Morezuelas ◽  
Nestor Alor ◽  
Leire Barandalla ◽  
Enrique Ritter
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Genetic Changes ◽  
Detached Leaves ◽  
Severity Of The Disease ◽  
Pathogen Populations ◽  
Incompatibility Reactions

The oomycete Phytophthora infestans is responsible for the disease known as late blight in potato and tomato. It is the plant pathogen that has caused the greatest impact on humankind so far and, despite all the studies that have been made, it remains the most important in this crop. In Spain during the last years a greater severity of the disease has been observed in both, potato and tomato, probably due to genetic changes in pathogen populations described recently. The aim of this study was the characterization of the physiological strains of 52 isolates of P. infestans obtained in different potato-growing areas in Spain. For this purpose, inoculations on detached leaves were performed in order to determine compatibility or incompatibility reactions. A total of 17 physiological races were found. The less frequent virulence factors were Avr5 and Avr8. By studying the epidemiology of the pathogen, a specific breeding program for late blight resistance can be implemented.

Allele Mining in Solanum Germplasm: Cloning and Characterization of RB-Homologous Gene Fragments from Late Blight Resistant Wild Potato Species

2015 ◽  
Vol 33 (5) ◽  
pp. 1584-1598 ◽  
Author(s):  
Jagesh Kumar Tiwari ◽  
Sapna Devi ◽  
Sanjeev Sharma ◽  
Poonam Chandel ◽  
Shashi Rawat ◽  
...  
Keyword(s):  
Late Blight ◽  
Homologous Gene ◽  
Wild Potato ◽  
Allele Mining ◽  
Wild Potato Species ◽  
Potato Species

scholarly journals Competition between Phytophthora infestans Effectors Leads to Increased Aggressiveness on Plants Containing Broad-Spectrum Late Blight Resistance

PLoS ONE ◽  
2010 ◽  
Vol 5 (5) ◽  
pp. e10536 ◽  
Author(s):  
Dennis A. Halterman ◽  
Yu Chen ◽  
Jiraphan Sopee ◽  
Julio Berduo-Sandoval ◽  
Amilcar Sánchez-Pérez
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Broad Spectrum ◽  
Late Blight Resistance ◽  
Blight Resistance

scholarly journals Performance of Transgenic Potato Containing the Late Blight Resistance Gene RB

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Dennis A. Halterman ◽  
Lara Colton Kramer ◽  
Susan Wielgus ◽  
Jiming Jiang
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Transgenic Potato ◽  
Field Trials ◽  
Blight Resistance ◽  
Solanum Bulbocastanum ◽  
Wild Potato Species ◽  
Foliar Resistance ◽  
In The Wild

Late blight of potato, caused by Phytophthora infestans, is one of the most devastating diseases of potato. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum through map-based cloning. The full-length gene coding sequence, including the open reading frame and promoter, has been integrated into cultivated potato (S. tuberosum) using Agrobacterium-mediated transformation. RB-containing transgenic plants were challenged with P. infestans under optimal late blight conditions in greenhouse experiments. All transgenic lines containing RB exhibited strong foliar resistance. Field-grown transgenic tubers also were tested for resistance to P. infestans. In contrast to the foliar resistance phenotype, RB-containing tubers did not exhibit increased resistance. Two years of field trials were used to ascertain whether the presence of RB had any effect on tuber yield. We were unable to detect any significant effect on tuber size or yield after addition of the resistance gene to several S. tuberosum cultivars.

scholarly journals The Late Blight Resistance Locus Rpi-blb3 from Solanum bulbocastanum Belongs to a Major Late Blight R Gene Cluster on Chromosome 4 of Potato

2005 ◽  
Vol 18 (7) ◽  
pp. 722-729 ◽  
Author(s):  
Tae-Ho Park ◽  
Jack Gros ◽  
Anne Sikkema ◽  
Vivianne G. A. A. Vleeshouwers ◽  
Marielle Muskens ◽  
...  
Keyword(s):  
Late Blight ◽  
Gene Cluster ◽  
Late Blight Resistance ◽  
Gene Clusters ◽  
Blight Resistance ◽  
Resistance Locus ◽  
R Gene ◽  
Chromosome 4 ◽  
Solanum Bulbocastanum ◽  
Wild Potato Species

Late blight, caused by Phytophthora infestans, is one of the most devastating diseases in cultivated potato. Breeding of new potato cultivars with high levels of resistance to P. infestans is considered the most durable strategy for future potato cultivation. In this study, we report the identification of a new late-blight resistance (R) locus from the wild potato species Solanum bulbocastanum. Using several different approaches, a high-resolution genetic map of the new locus was generated, delimiting Rpi-blb3 to a 0.93 cM interval on chromosome 4. One amplification fragment length polymorphism marker was identified that cosegregated in 1,396 progeny plants of an intraspecific mapping population with Rpi-blb3. For comparative genomics purposes, markers linked to Rpi-blb3 were tested in mapping populations used to map the three other late-blight R loci Rpi-abpt, R2, and R2-like also to chromosome 4. Marker order and allelic conservation suggest that Rpi-blb3, Rpiabpt, R2, and R2-like reside in the same R gene cluster on chromosome 4 and likely belong to the same gene family. Our findings provide novel insights in the evolution of R gene clusters conferring late-blight resistance in Solanum spp.

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