Biophysical and biochemical mechanism influencing shoot and fruit borer tolerance in brinjal genotypes

The phenomenon of anoxic phosphate uptake with simultaneous denitrification was studied. For this purpose kinetic batch tests have been carried out by using the activated sludge samples from three modifications of nutrient removal activated sludge systems: two based on an anaerobic-anoxic-oxic (A2/O) system and a third on an anaerobic-oxic (A/O) system. The results showed significant differences in anoxic phosphate uptake rate between activated sludge which was alternatively exposed to anoxic conditions and activated sludge from the A/O arrangement. These differences were also accompanied by different denitrification rates. Simultaneously with batch experiments the microscopic observation of activated sludge samples was carried out. Neisser and Gram stained samples showed clear differences in shape, size and distribution of polyphosphate accumulating bacteria between A2/O and A/O Processes. Moreover, experiments performed using genetic probes confirmed the differences in microbiological composition of activated sludge samples from different nutrient removal system arrangements.

Download Full-text

Apoptotic Effects of N-(2-hydroxyphenyl)-2-propylpentanamide on U87-MG and U-2 OS Cells and Antiangiogenic Properties

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200728125356 ◽

2020 ◽

Vol 20 ◽

Author(s):

Paola Castillo-Juárez ◽

Sebastián C. Sanchez ◽

Alma D. Chávez-Blanco ◽

Humberto Mendoza-Figueroa ◽

José Correa-Basurto

Keyword(s):

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Histone Deacetylases ◽

Biochemical Mechanism ◽

Antiproliferative Effects ◽

Human Osteosarcoma ◽

Antiproliferative Agent ◽

Induced Apoptosis ◽

Apoptotic Effects

Background and Objective: Histone deacetylases (HDACs) are important therapeutic targets for many types of human cancers. A derivative of valproic acid, N-(2-hydroxyphenyl)-2-propylpentanamide (HO-AAVPA), has antiproliferative properties on some cancer cell lines and inhibits the HDAC1 isoform. Materials and Methods: In this work, HO-AAVPA was tested as an antiproliferative agent in U87-MG (human glioblastoma) and U-2 OS cells (human osteosarcoma), which are types of cancer that are difficult to treat, and its antiangiogenic properties were explored. Results: HO-AAVPA had antiproliferative effects at 48 h with an IC50 = 0.655 mM in U87-MG cells and an IC50 = 0.453 mM in U-2 OS cells. Additionally, in the colony formation assay, HO-AAVPA decreased the number of colonies by approximately 99% in both cell lines and induced apoptosis by 31.3% in the U-2 OS cell line and by 78.2% in the U87-MG cell line. Additionally, HO-AAVPA reduced the number of vessels in chorioallantoid membranes (CAMs) by approximately 67.74% and IL-6 levels in both cell lines suggesting that the biochemical mechanism on cancer cell of HO-AAVPA is different compared to VPA. Conclusion: HO-AAVPA has antiproliferative effects on glioblastoma and osteosarcoma and antiangiogenic properties.

Download Full-text

Appraisal of resistant genotypes against brinjal shoot and fruit borer (BSFB), Leucinodes orbonalis, Guenee

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v16i2.37965 ◽

2018 ◽

Vol 16 (2) ◽

pp. 227-231

Author(s):

Hafija Sultana ◽

Md Aabdul Mannan ◽

Md Mostofa Kamal ◽

Khan Golam Quddus ◽

Shimul Das

Keyword(s):

Pot Experiment ◽

Leucinodes Orbonalis ◽

Fruit Borer ◽

Three Stages ◽

Fruit Damage ◽

Shoot Damage ◽

Resistant Genotypes

The main purpose of this study was to find out the best variety of brinjal having resistance to BSFB. A pot experiment was carried out at the germplasm centre, Agrotechnology Discipline, Khulna University, Khulna during February 2014 to August 2014. The experiment was laid out in CRD using thirteen varieties with five replications. The varieties were V1=BARI begun-1, V2=BARI begun-4, V3=BARI begun-5, V4=BARI begun-6, V5=BARI begun-7, V6=BARI begun-8, V7=BARI begun-9, V8=BARI begun-10, V9=Makra, V10=Muktokashi, V11=Lalita, V12=Hazra, V13=Chaga. Data were collected on total number of shoot, total number of infested shoot, percentage of shoot damage, percentage of shoot infestation reduction, numbers of larvae per infested shoot, total number of fruit, percentage of fruit damage, percentage of fruit infestation reduction, numbers of larvae per infested fruit. Results have shown that the minimum shoot infestation was found in BARI begun-6 (29.60%, 32.40%, 29.86% and 29.38%, respectively at four different stages of eggplant). Percent of fruit infestation was minimum in V4 (BARI begun-6) (25.16%, 27.42% and 25.40% at three stages respectively).J. Bangladesh Agril. Univ. 16(2): 227-231, August 2018

Download Full-text