In Vitro Efficacy of Fungicides on Sclerotinia sclerotiorum and their Potential for Control of Stem Rot in Indian Mustard (Brassica juncea L.)

Author(s):  
Shri Kishan Bairwa ◽  
Shanker Lal Godara ◽  
Susheela Meena
2014 ◽  
Vol 8 (36) ◽  
pp. 3295-3306
Author(s):  
Kishan Bairwa Shri ◽  
Lal Godara Shankar ◽  
Kumar Pardeep ◽  
Chand Bairwa Ramesh ◽  
Gangopadhyay Samiran

2016 ◽  
Vol 8 (1) ◽  
pp. 441-444
Author(s):  
Rakesh Rakesh ◽  
A.S. Rathi ◽  
Anil Kumar ◽  
Hawa Singh

The current investigation was carried out under -in vitro and under sick plot conditions of the Department of Plant Pathology, CCS Haryana Agricultural University, Hisar to test the efficacy of different fungicides against Sclerotinia sclerotiorum. The experiment was carried out through poison food technique under in vitro conditions andthrough foliar sprays under sick plot conditions. Efficacy of fungicides in vitro against S. sclerotiorum showed that carbendazim and hexaconazole completely inhibited mycelial growth up to 100 per cent at 50 ppm concentration. Propiconazole inhibited 96.39 per cent mycelial growth at 50 ppm and 100 per cent at 1000 ppm, while tricyclazole failed to inhibit mycelial growth up to 100 ppm, however, at 1000 ppm concentration, there was 100 per cent mycelial inhibition. Mancozeb and captan completely inhibited up to 100 per cent mycelial growth at 500 ppm concentration. Copper oxychloride and pencycuron were not effective at lower concentrations but had inhibited 45.28 and 22.50 per cent mycelial growth at 1000 ppm. Field experiment was conducted to test the efficacy of those fungicides which were found best under in vitro conditions. All the four fungicides tested significantly controlled Sclerotinia stem rot disease and increased the seed yield as compared to untreated control. Two Foliar sprays with hexaconazole @ 0.05% at 45 and 60 days after sowing was found most effective in controlling disease incidence up to 78.3 per cent and increasing seed yield up to 37.6 per cent as compared to untreated control.


2016 ◽  
Vol 1 (02) ◽  
pp. 190-193
Author(s):  
Jhilmil Gupta

Sclerotinia sclerotiorum (Lib.) de Bary is a soil borne pathogen capable of infecting more than 400 host plants worldwide. It is a major pathogen that plays a crucial role in reducing the yield in economically important crops. The capability of sclerotia to survive for more than 4 years becomes very difficult to manage the crop from the infection of Sclerotinia rot fungus. Stem rot of indian mustard [Brassica juncea (L.) Czern and Cross] caused by Sclerotinia sclerotiorum is potentially a serious threat in many mustard growing areas in India. Treatments of seeds and foliar spray with of fungicides applied at regular intervals are effective in reducing infection, but uses of chemicals are hazardous, harmful for beneficial micro-organisms. Biological control of plant pathogens offers an exciting opportunity to manage plant diseases. In the present study, the efficacy of four bio-agents, viz., Coniothyrium minitans, Aspergilus nidulans, Trichoderma harzianum, and Pseudomonas chlororaphis were evaluated for the control of stem rot of Indian mustard. Results on bio-efficacy of different bioagents, when evaluated under glass house condition, the Coniothyrium minitans was the most effective agent and caused highest reduction (64.7 %) in carpogenic germination of sclerotia followed by Aspergilus nidulans (52.5 %) and Trichoderma harzianum (48.8 %), over control while Pseudomonas chlororaphis (48.3 %) was at par with T. harzianum. All the bioagents showed significant reduction effective in controlling the disease. Similar results were achieved when bioagents tested on dual inoculated plates.


Plant Disease ◽  
2015 ◽  
Vol 99 (7) ◽  
pp. 969-975 ◽  
Author(s):  
Congying Xu ◽  
Xiaoyu Liang ◽  
Yiping Hou ◽  
Mingguo Zhou

We determined the effects and efficacy of benzothiostrobin, a new strobilurin-derived fungicide, against the plant-pathogenic fungus Sclerotinia sclerotiorum (the causal agent of Sclerotinia stem rot). Mycelial growth and sclerotial germination in vitro were strongly inhibited by benzothiostrobin in the presence of salicylhydroxamic acid. On detached rapeseed leaves, benzothiostrobin at 40 μg/ml reduced lesion development by 87%. No cross-resistance was detected between benzothiostrobin and carbendazim, iprodione, fludioxonil, or boscalid. A formulated mixture of benzothiostrobin and fluazinam at 1:1 had synergistic activity against S. sclerotiorum in vitro. In field trials, benzothiostrobin alone or formulated with fluazinam at 1:1 (150 g a.i. ha−1) was significantly (P < 0.05) superior to iprodione in controlling Sclerotinia stem rot of rapeseed. These results suggest that benzothiostrobin has substantial potential for the control of Sclerotinia stem rot.


2017 ◽  
Vol 23 (1) ◽  
Author(s):  
PRAMOD KUMAR FATEHPURIA ◽  
RAJNI S. SASODE ◽  
DEVASHISH R. CHOBE ◽  
VEERENDRA GUPTA ◽  
REETI SINGH

Indian mustard, Brassica juncea belong to Brassicaceae and Centre of origin Mideterian. Sclerotinia stem rot/ blight caused by Sclerotinia sclerotiorum is one of the most important fungal diseases in rapeseed and other crops in the world. The present study was undertaken to access the forty seven germplasm/varieties to find out the resistance against S. sclerotiorum under inoculated condition. The reaction of forty-seven germplasm was evaluated in randomized complete block design with thrice replications at the experimental field, Department of Plant Pathology, College of Agriculture, Gwalior during Rabi season 2014-15 and laboratory work was done in the Department of Plant Pathology, College of Agriculture, Gwalior (M.P.). Observations for severity of stem rot on main stem were recorded after 120 days of sowing using 0 - 4 scale. (Assessment scale 0-4 where 0 = no disease (resistance), 1 = less than half stem girdled (moderate resistance), 2 = more than half stem girdled (susceptible), 3 = whole stem girdled (moderate susceptible), 4 = plant dead (highly suscepitble). Forty-seven germplasm/varieties were evaluated for their reaction against Sclerotinia blight (Sclerotinia sclerotiorum). Out of forty seven germplasm / varieties, tested two Cultivar SBG-14-5 and SBG-14- 6 gave highly resistance reactions, while twenty six varieties are found resistant SBG-14-2, SBG-14-3, SBG-14-4, SBG-14-7, SBG-14-8, SBG-14-9, SBG-14-10, SBG-14-11, SBG-14-13, SBG14-15, SBG-14-17, SBG-14-18, SBG-14-23, SBG-14-27, SBG-14-28, SBG-14-30, SBG-14-31, SBG-14-32, SBG-14-33, SBG-14-34, SBG-14-35, SBG-14-36, SBG- 14-41, SBG-14-42, NDNSR-14-76, NDNSR-14-77, twelve varieties are found susceptible SBG-14-1, SBG-14-12, SBG- 14-14, SBG-14-16, SBG-14-21, SBG-14-22, SBG-14-24, SBG-14-26, SBG-14-29, SBG-14-37, SBG-14-39, SBG-14-40 and seven varieties SBG-14-19, SBG-14-20, SBG-14-25, SBG-14-38, Varuna, Rohini and BIOYSR were found highly susceptible against Sclerotinia blight (Sclerotinia sclerotiorum).


2014 ◽  
Vol 8 (42) ◽  
pp. 3650-3655
Author(s):  
Kishan Bairwa Shri ◽  
Lal Godara Shankar ◽  
Meena Susheela ◽  
Kumar Jatav Narendra ◽  
Chand Bairwa Ramesh

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