scholarly journals Effects of a Forebrain Embryonic Zinc Finger-Like p.Gly105(12_13) polymorphism on mastitis resistance: an embryo-transfer study

2013 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Mayumi Sugimoto ◽  
Michiko Uchiza ◽  
Masatoshi Kuniyuki
1994 ◽  
Vol 72 (3) ◽  
pp. 725-731 ◽  
Author(s):  
C. R. Youngs ◽  
L. K. Christenson ◽  
S. P. Ford

1992 ◽  
Vol 70 (8) ◽  
pp. 2349-2358 ◽  
Author(s):  
T. M. Wilken ◽  
L. L. Lo ◽  
D. G. McLaren ◽  
R. L. Fernando ◽  
P. J. Dziuk

2008 ◽  
Vol 38 (6) ◽  
pp. 579-595 ◽  
Author(s):  
Markus Wöhr ◽  
Maik Dahlhoff ◽  
Eckhard Wolf ◽  
Florian Holsboer ◽  
Rainer K. W. Schwarting ◽  
...  

1996 ◽  
Vol 8 (8) ◽  
pp. 1123 ◽  
Author(s):  
CM Dwyer ◽  
AB Lawrence ◽  
HE Brown ◽  
G Simm

To distinguish between ewe and lamb breed effects on prenatal growth, ease of parturition and early lamb behaviour, an embryo-transfer study was carried out using a hill breed (Scottish Blackface; liveweight: 54.25 +/- 1.03 kg, mean +/- s.e.m.) and a lowland breed (Suffolk; 80.33 +/- 1.52 kg) to obtain the four possible combinations of ewe and lamb. Data were collected from 38 Blackface ewes (18 with Blackface lambs and 20 with Suffolk lambs) and 41 Suffolk ewes (20 with Blackface lambs and 21 with Suffolk lambs); all ewes were given single embryos. Suffolk lambs had a significantly longer gestation than Blackface lambs (1.5 days, P < 0.01), regardless of ewe breed. Suffolk lambs also had a longer labour (20 min, P < 0.05) and were significantly more likely to require birth assistance (17/21, 81% of all assisted deliveries; P < 0.001), as were male lambs (19/21, 90%; P < 0.01). These variables were independent of ewe breed. Blackface lambs were significantly more active than Suffolk lambs in the first 2 h after birth; ewe breed had little effect on lamb behaviour. Blackface lambs stood twice as quickly as Suffolk lambs after birth (13 min v. 24 min; P < 0.001), and were significantly more likely to suckle within the first 2 h after birth (92% v. 66%; P < 0.05). The behavioural retardation of Suffolk lambs may be a consequence of their birth difficulty which increases their likelihood of suffering birth trauma and hypoxia at parturition. Together, these factors may increase the probability of neonatal death in these lambs.


2021 ◽  
Vol 116 (3) ◽  
pp. e16-e17
Author(s):  
Nicole M. Marchetto ◽  
Barry E. Perlman ◽  
David Kulak ◽  
Victoria Boden ◽  
Sara S. Morelli ◽  
...  

2014 ◽  
Vol 26 (1) ◽  
pp. 225
Author(s):  
T. Kaneko ◽  
T. Mashimo

The rat has been used as an important animal for understanding human diseases. Genetically engineered rat strains are used as a human disease model in various research fields. Genetically engineered rat strains are now being routinely produced, not only as transgenic animals but also using gene knockout techniques. Recently, zinc finger nucleases (ZFN) and TAL effector nucleases (TALEN) have enabled editing targeted genes without using embryonic stem cells. These techniques have been applied for production of the knockout and knockin animals. We here studied that the effects of gene targeting by ZFN and TALEN introduced into rat embryos for efficient production of knockout rats. We custom-designed ZFN and TALEN plasmids targeted rat interleukin 2 receptor gamma (Il2rg) gene. Each mRNA was transcribed in vitro from these plasmids. Final concentration of mRNA was adjusted at 10 ng μL–1 in sterilized water for microinjection. Messenger RNA was injected into rat pronuclear stage embryos. The embryos were then cultured in vitro to the 2-cell stage, and were transferred into oviducts of pseudopregnant females. The rate of development of offspring of embryos and effects of editing targeted genes were examined. Of 41 two-cell embryos introduced ZFN after embryo transfer, 9 embryos (22%) developed to offspring. Three offspring (33%) had an edited targeted gene locus. In the embryos introduced TALEN, 30% (6 offspring) of embryos developed to offspring after embryo transfer and all offspring had an edited targeted gene locus. This study demonstrated that the ZFN and TALEN mRNA was active after introduction into rat embryos. Knockout rats could be produced by introduction of ZFN and TALEN into rat embryos. ZFN and TALEN will provide a powerful new approach for targeted gene editing not only in rats but also in other animal species.


1979 ◽  
Vol 48 (2) ◽  
pp. 298-306 ◽  
Author(s):  
Lori A. Hinkelman ◽  
G. E. Bradford ◽  
E. J. Pollak ◽  
G. B. Anderson ◽  
P. T. Cupps

2013 ◽  
Vol 100 (3) ◽  
pp. S292 ◽  
Author(s):  
N. Kawakami ◽  
Y. Aoi ◽  
C. Shindou ◽  
H. Saito ◽  
R. Hirata ◽  
...  

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