scholarly journals Peer Review #2 of "Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata (v0.2)"

2017 ◽  
Keyword(s):  
Peer Review ◽  
Dna Markers ◽  
Montipora Capitata

scholarly journals Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

2017 ◽  
Author(s):  
Hollie Putnam ◽  
Diane Adams ◽  
Ehud Zelzion ◽  
Nicole Wagner ◽  
Huan Qiu ◽  
...  
Keyword(s):  
Dna Markers ◽  
Sequence Variation ◽  
Marker Gene ◽  
Genomic Data ◽  
Single Copy ◽  
Rdna Repeat ◽  
Montipora Capitata ◽  
Sperm Bundle ◽  
Limited Variation ◽  
A Minor

We investigated intra and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.

scholarly journals Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3319 ◽  
Author(s):  
Hollie M. Putnam ◽  
Diane K. Adams ◽  
Ehud Zelzion ◽  
Nicole E. Wagner ◽  
Huan Qiu ◽  
...  
Keyword(s):  
Dna Markers ◽  
Sequence Variation ◽  
Marker Gene ◽  
Genomic Data ◽  
Single Copy ◽  
Rdna Repeat ◽  
Montipora Capitata ◽  
Sperm Bundle ◽  
Limited Variation ◽  
A Minor

We investigated intra- and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2–3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.

scholarly journals Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

2017 ◽  
Author(s):  
Hollie Putnam ◽  
Diane Adams ◽  
Ehud Zelzion ◽  
Nicole Wagner ◽  
Huan Qiu ◽  
...  
Keyword(s):  
Dna Markers ◽  
Sequence Variation ◽  
Marker Gene ◽  
Genomic Data ◽  
Single Copy ◽  
Rdna Repeat ◽  
Montipora Capitata ◽  
Sperm Bundle ◽  
Limited Variation ◽  
A Minor

We investigated intra and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.

Sign in / Sign up

Export Citation Format

Share Document