Limited variation between SARS-CoV-2-infected individuals in domain specificity and relative potency of the antibody response against the spike glycoprotein

The spike protein of SARS-CoV-2 is arranged as a trimer on the virus surface, composed of three S1 and three S2 subunits. Infected and vaccinated individuals generate antibodies against spike, which can neutralize the virus. Most antibodies target the receptor-binding domain (RBD) and N-terminal domain (NTD) of S1; however, antibodies against other regions of spike have also been isolated. The variation between infected individuals in domain specificity of the antibodies and in their relative neutralization efficacy is still poorly characterized. To this end, we tested serum and plasma samples from 85 COVID-19 convalescent subjects using 7 immunoassays that employ different domains, subunits and oligomeric forms of spike to capture the antibodies. Samples were also tested for their neutralization of pseudovirus containing SARS-CoV-2 spike and of replication-competent SARS-CoV-2. We observed strong correlations between the levels of NTD- and RBD-specific antibodies, with a fixed ratio of each type to all anti-spike antibodies. The relative potency of the response (defined as the measured neutralization efficacy relative to the total level of spike-targeting antibodies) also exhibited limited variation between subjects, and was not associated with the overall amount of anti-spike antibodies produced. Accordingly, the ability of immunoassays that use RBD, NTD and different forms of S1 or S1/S2 as capture antigens to estimate the neutralizing efficacy of convalescent samples was largely similar. These studies suggest that host-to-host variation in the polyclonal response elicited against SARS-CoV-2 spike is primarily limited to the quantity of antibodies generated rather than their domain specificity or relative neutralization potency.

Combined In Situ Chemical and Sr Isotopic Compositions and U–Pb Ages of the Mushgai Khudag Alkaline Complex: Implications of Immiscibility, Fractionation, and Alteration

The Mushgai Khudag complex consists of numerous silicate volcanic-plutonic rocks including melanephelinites, theralites, trachytes, shonkinites, and syenites and also hosts numerous dykes and stocks of magnetite-apatite-enriched rocks and carbonatites. It hosts the second largest REE–Fe–P–F–Sr–Ba deposit in Mongolia, with REE mineralization associated with magnetite-apatite-enriched rocks and carbonatites. The bulk rock REE content of these two rock types varies from 21,929 to 70,852 ppm, which is much higher than that of syenites (716 ± 241 ppm). Among these, the altered magnetite-apatite-enriched rocks are characterized by the greatest level of REE enrichment (58,036 ± 13,313 ppm). Magmatic apatite from magnetite-apatite-enriched rocks is commonly euhedral with purple luminescence, and altered apatite displays variable purple to blue luminescence and shows fissures and hollows with deposition of fine-grained monazite aggregates. Most magmatic apatite within syenite is prismatic and displays oscillatory zoning with variable purple to yellow luminescence. Both magmatic and altered apatite from magnetite-apatite-enriched rocks were dated using in situ U–Pb dating and found to have ages of 139.7 ± 2.6 and 138.0 ± 1.3 Ma, respectively, which supports the presence of late Mesozoic alkaline magmatism. In situ 87Sr/86Sr ratios obtained for all types of apatite and calcite within carbonatite show limited variation (0.70572–0.70648), which indicates derivation from a common mantle source. All apatite displays steeply fractionated chondrite-normalized REE trends with significant LREE enrichment (46,066 ± 71,391 ppm) and high (La/Yb)N ratios ranging from 72.7 to 256. REE contents and (La/Yb)N values are highly variable among different apatite groups, even within the same apatite grains. The variable REE contents and patterns recorded by magmatic apatite from the core to the rim can be explained by the occurrence of melt differentiation and accompanying fractional crystallization. The Y/Ho ratios of altered apatite deviate from the chondritic values, which reflects alteration by hydrothermal fluids. Altered apatite contains a high level of REE (63,912 ± 31,785 ppm), which are coupled with increased sulfur and/or silica contents, suggesting that sulfate contributes to the mobility and incorporation of REEs into apatite during alteration. Moreover, altered apatite is characterized by higher Zr/Hf, Nb/Ta, and (La/Yb)N ratios (179 ± 48, 19.4 ± 10.3, 241 ± 40, respectively) and a lack of negative Eu anomalies compared with magmatic apatite. The distinct chemical features combined with consistent Sr isotopes and ages for magmatic and altered apatite suggest that pervasive hydrothermal alterations at Mushgai Khudag are most probably being induced by carbonatite-evolved fluids almost simultaneously after the alkaline magmatism.

Distribution of Prophages in the Oenococcus oeni Species

Oenococcus oeni is the most exploited lactic acid bacterium in the wine industry and drives the malolactic fermentation of wines. Although prophage-like sequences have been identified in the species, many are not characterized, and a global view of their integration and distribution amongst strains is currently lacking. In this work, we analyzed the complete genomes of 231 strains for the occurrence of prophages, and analyzed their size and positions of insertion. Our data show the limited variation in the number of prophages in O. oeni genomes, and that six sites of insertion within the bacterial genome are being used for site-specific recombination. Prophage diversity patterns varied significantly for different host lineages, and environmental niches. Overall, the findings highlight the pervasive presence of prophages in the O. oeni species, their role as a major source of within-species bacterial diversity and drivers of horizontal gene transfer. Our data also have implications for enhanced understanding of the prophage recombination events which occurred during evolution of O. oeni, as well as the potential of prophages in influencing the fitness of these bacteria in their distinct niches.

Time perception and patience: individual differences in interval timing precision predict choice impulsivity in European starlings, Sturnus vulgaris

Impulsivity, in the sense of the extent rewards are devalued as the time until their realization increases, is linked to various negative outcomes in humans, yet understanding of the cognitive mechanisms underlying it is limited. Variation in the imprecision of interval timing is a possible contributor to variation in impulsivity. We use a numerical model to generate predictions concerning the effect of timing imprecision on impulsivity. We distinguish between fixed imprecision (the imprecision that applies even when timing the very shortest time intervals) and proportional imprecision (the rate at which imprecision increases as the interval becomes longer). The model predicts that impulsivity should increase with increasing fixed imprecision, but decrease with increasing proportional imprecision. We present data from a cohort of European starlings (Sturnus vulgaris, n = 28) in which impulsivity had previously been measured through an intertemporal choice paradigm. We tested interval timing imprecision in the same individuals using a tri-peak temporal reproduction procedure. We found repeatable individual differences in both fixed and proportional imprecision. As predicted, birds with greater proportional imprecision in interval timing made fewer impulsive choices, whilst those with greater fixed imprecision tended to make more. Contradictory observations in the literature regarding the direction of association between timing imprecision and impulsivity might be clarified by distinguishing between fixed and proportional components of imprecision.

Analisis Kerajinan Boneka Dari Limbah Karung Goni Dan Kain PercaDitinjau DariGesture

This study aims to describe the cues on dolls made of jute sack waste and patchwork as crafts. The data study techniques in this study were observation, documentation and interviews. The total population of the study was 15 dolls in the doll craftsmen. The sampling technique of this researcher was total sampling, which was taken from the total population who drew doll crafts from gunny sacks and patchwork. The results showed that there was limited variation in facial expressions in doll products. Gestures on dolls are neat and sturdy but have elastic properties so that the movements of the dolls show character in the activity. In addition to the unique shape and color produced from the patchwork, it is a center point or a great attraction to attract consumers' attention. Furthermore, in making dolls from jute sack waste and patchwork, it is unique in its creation which is used as a reference for making a doll product, which has certain characteristics, for example the results of this doll have characteristics related to human activity.

Preschool Children’s Learning Opportunities Using Natural Numbers in Number Row Activities

This study analysed how preschool teachers differently enacted the same mathematical activity for preschool children to discern numbers, and how this affected the children’s learning opportunities during the activity. The analysis was based on variation theory and Chi’s taxonomy of learning activities. Two Swedish preschool teachers’ enactment of the same mathematical activity for 27 children aged 4–6 years was studied. Video recordings of what the children were offered to discern were used in the analysis. The results indicate that variations in how the teachers chose to enact the activity produced two different learning opportunities for the children. Differences in what aspects were made discernible were closely linked to the characteristics of the activity implemented. The enactments differed even if the same game was chosen and the same amount of time was used in the play-based activity. In one preschool group, there were few opportunities to discern more than the nominal form of numbers; the other preschool group had an activity focused on all number forms simultaneously. In addition, in the latter group, the children had the opportunity to develop equinumerosity. The results suggest that the activity with limited variation was more appropriate for learning with undeveloped knowledge; the children with more developed understanding required a more varied design. This study contributes to the knowledge of how the design of an activity affects children’s learning differently, which is important when planning learning-based preschool activities.

Pug-Headedness Anomaly in a Wild and Isolated Population of Native Mediterranean Trout Salmo trutta L., 1758 Complex (Osteichthyes: Salmonidae)

Skeletal anomalies are commonplace among farmed fish. The pug-headedness anomaly is an osteological condition that results in the deformation of the maxilla, pre-maxilla, and infraorbital bones. Here, we report the first record of pug-headedness in an isolated population of the critically endangered native Mediterranean trout Salmo trutta L., 1758 complex from Sardinia, Italy. Fin clips were collected for the molecular analyses (D-loop, LDH-C1* locus. and 11 microsatellites). A jaw index (JI) was used to classify jaw deformities. Ratios between the values of morphometric measurements of the head and body length were calculated and plotted against values of body length to identify the ratios that best discriminated between malformed and normal trout. Haplotypes belonging to the AD lineage and the genotype LDH-C1*100/100 were observed in all samples, suggesting high genetic integrity of the population. The analysis of 11 microsatellites revealed that observed heterozygosity was similar to the expected one, suggesting the absence of inbreeding or outbreeding depression. The frequency of occurrence of pug-headedness was 12.5% (two out of 16). One specimen had a strongly blunted forehead and an abnormally short upper jaw, while another had a slightly anomaly asymmetrical jaw. Although sample size was limited, variation in environmental factors during larval development seemed to be the most likely factors to trigger the deformities.

The aerodynamic optimisation of a low-Reynolds paper plane with adjoint method

ABSTRACT The aerodynamic performance of a deployable and low-cost unmanned aerial vehicle (UAV) is investigated and improved in present work. The parameters of configuration, such as airfoil and winglet, are determined via an optimising process based on a discrete adjoint method. The optimised target is locked on an increasing lift-to-drag ratio with a limited variation of pitching moments. The separation that will lead to a stall is delayed after optimisation. Up to 128 design variables are used by the optimised solver to give enough flexibility of the geometrical transformation. As much as 20% enhancement of lift-to-drag ratio is gained at the cruise angle-of-attack, that is, a significant improvement in the lift-to-drag ratio adhering to the preferred configuration is obtained with increasing lift and decreasing drag coefficients, essentially entailing an improved aerodynamic performance.

We are what we click: Understanding time and content-based habits of online news readers

The article contributes both conceptually and methodologically to the study of online news consumption by introducing new approaches to measuring user information behaviour and proposing a typology of users based on their click behaviour. Using as a case study two online outlets of large national newspapers, it employs computational approaches to detect patterns in time- and content-based user interactions with news content based on clickstream data. The analysis of interactions detects several distinct timelines of news consumption and scrutinises how users switch between news topics during reading sessions. Using clustering analysis, the article then identifies several types of news readers (e.g. samplers, gourmets) and examines their news diets. The results point out the limited variation in topical composition of the news diets between different types of readers and the tendency of these diets to align with the news supply patterns (i.e. the average distribution of topics covered by the outlet).