scholarly journals Peer Review #1 of "Toxicity of Melaleuca alternifolia essential oil to the mitochondrion and NAD+/NADH dehydrogenase in Tribolium confusum (v0.1)"

2018 ◽  
Keyword(s):  
Essential Oil ◽  
Peer Review ◽  
Nadh Dehydrogenase ◽  
Tribolium Confusum ◽  
Melaleuca Alternifolia

scholarly journals Toxicity of Melaleuca alternifolia essential oil to the mitochondrion and NAD+/NADH dehydrogenase in Tribolium confusum

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5693 ◽  
Author(s):  
Min Liao ◽  
Qian-Qian Yang ◽  
Jin-Jing Xiao ◽  
Yong Huang ◽  
Li-Jun Zhou ◽  
...  
Keyword(s):  
Essential Oil ◽  
Respiratory Chain ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Respiratory Chain ◽  
Tribolium Confusum ◽  
Melaleuca Alternifolia ◽  
Insecticidal Effect ◽  
Stored Product Pest ◽  
Insecticidal Efficacy ◽  
Mitochondrial Respiratory

Background In our previous study, Melaleuca alternifolia essential oil (EO) was considered to have an insecticidal effect by acting on the mitochondrial respiratory chain in insects. However, the mode of action is not fully understood. Methods In this study, we investigated the insecticidal efficacy of the M. alternifolia EO against another major stored-product pest, Tribolium confusum Jacquelin du Val. Rarefaction and vacuolization of the mitochondrial matrix were evident in oil-fumigated T. confusum adults. Results Alterations to the mitochondria confirmed the insecticidal effect of the M. alternifolia EO. Furthermore, comparative transcriptome analysis of T. confusum using RNA-seq indicated that most of the differentially expressed genes were involved in insecticide detoxification and mitochondrial function. The biochemical analysis showed that the intracellular NAD+/NADH ratio is involved in the differential effect of the M. alternifolia EO. Discussion These results led us to conclude that NAD+/NADH dehydrogenase may be the prime target site for the M. alternifolia EO in insects, leading to blocking of the mitochondrial respiratory chain.

scholarly journals Toxicity of Melaleuca alternifolia essential oil to the mitochondrion and NAD+/NADH dehydrogenase in Tribolium confusum

2018 ◽  
Author(s):  
Min Liao ◽  
Qian-Qian Yang ◽  
Jin-Jing Xiao ◽  
Yong Huang ◽  
Li-Jun Zhou ◽  
...  
Keyword(s):  
Essential Oil ◽  
Respiratory Chain ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Respiratory Chain ◽  
Tribolium Confusum ◽  
Melaleuca Alternifolia ◽  
Insecticidal Effect ◽  
Stored Product Pest ◽  
Insecticidal Efficacy ◽  
Mitochondrial Respiratory

Background. In our previous study, Melaleuca alternifolia essential oil (EO) was considered to have an insecticidal effect by acting on the mitochondrial respiratory chain in insects. However, the mode of action is not fully understood. Methods. In this study, we investigated the insecticidal efficacy of the M. alternifolia EO against another major stored-product pest, Tribolium confusum Jacquelin du Val. Rarefaction and vacuolization of the mitochondrial matrix were evident in oil-fumigated T. confusum adults. Results. Alterations to the mitochondria confirmed the insecticidal effect of the M. alternifolia EO. Furthermore, comparative transcriptome analysis of T. confusum using RNA-seq indicated that most of the differentially expressed genes were involved in insecticide detoxification and mitochondrial function. The biochemical analysis showed that the intracellular NAD+/NADH ratio is involved in the differential effect of the M. alternifolia EO. Discussion. These results led us to conclude that NAD+/NADH dehydrogenase may be the prime target site for the M. alternifolia EO in insects, leading to blocking of the mitochondrial respiratory chain.

scholarly journals Toxicity of Melaleuca alternifolia essential oil to the mitochondrion and NAD+/NADH dehydrogenase in Tribolium confusum

2018 ◽  
Author(s):  
Min Liao ◽  
Qian-Qian Yang ◽  
Jin-Jing Xiao ◽  
Yong Huang ◽  
Li-Jun Zhou ◽  
...  
Keyword(s):  
Essential Oil ◽  
Respiratory Chain ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Respiratory Chain ◽  
Tribolium Confusum ◽  
Melaleuca Alternifolia ◽  
Insecticidal Effect ◽  
Stored Product Pest ◽  
Insecticidal Efficacy ◽  
Mitochondrial Respiratory

Background. In our previous study, Melaleuca alternifolia essential oil (EO) was considered to have an insecticidal effect by acting on the mitochondrial respiratory chain in insects. However, the mode of action is not fully understood. Methods. In this study, we investigated the insecticidal efficacy of the M. alternifolia EO against another major stored-product pest, Tribolium confusum Jacquelin du Val. Rarefaction and vacuolization of the mitochondrial matrix were evident in oil-fumigated T. confusum adults. Results. Alterations to the mitochondria confirmed the insecticidal effect of the M. alternifolia EO. Furthermore, comparative transcriptome analysis of T. confusum using RNA-seq indicated that most of the differentially expressed genes were involved in insecticide detoxification and mitochondrial function. The biochemical analysis showed that the intracellular NAD+/NADH ratio is involved in the differential effect of the M. alternifolia EO. Discussion. These results led us to conclude that NAD+/NADH dehydrogenase may be the prime target site for the M. alternifolia EO in insects, leading to blocking of the mitochondrial respiratory chain.

scholarly journals Evaluation of Seven Essential Oils as Seed Treatments against Seedborne Fungal Pathogens of Cucurbita maxima

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2354
Author(s):  
Marwa Moumni ◽  
Mohamed Bechir Allagui ◽  
Kaies Mezrioui ◽  
Hajer Ben Amara ◽  
Gianfranco Romanazzi
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Fungal Pathogens ◽  
Seedling Emergence ◽  
Cucurbita Maxima ◽  
Melaleuca Alternifolia ◽  
Seedborne Pathogens ◽  
Origanum Majorana ◽  
Potential Use

Essential oils are gaining interest as environmentally friendly alternatives to synthetic fungicides for management of seedborne pathogens. Here, seven essential oils were initially tested in vivo for disinfection of squash seeds (Cucurbita maxima) naturally contaminated by Stagonosporopsis cucurbitacearum, Alternaria alternata, Fusarium fujikuro, Fusarium solani, Paramyrothecium roridum, Albifimbria verrucaria, Curvularia spicifera, and Rhizopus stolonifer. The seeds were treated with essential oils from Cymbopogon citratus, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and Origanum majorana (#1 and #2). Incidence of S. cucurbitacearum was reduced, representing a range between 67.0% in L. nobilis to 84.4% in O. majorana #2. Treatments at 0.5 mg/mL essential oils did not affect seed germination, although radicles were shorter than controls, except with C. citratus and O. majorana #1 essential oils. Four days after seeding, seedling emergence was 20%, 30%, and 10% for control seeds and seeds treated with C. citratus essential oil (0.5 mg/mL) and fungicides (25 g/L difenoconazole plus 25 g/L fludioxonil). S. cucurbitacearum incidence was reduced by ~40% for plantlets from seeds treated with C. citratus essential oil. These data show the effectiveness of this essential oil to control the transmission of S. cucurbitacearum from seeds to plantlets, and thus define their potential use for seed decontamination in integrated pest management and organic agriculture.

scholarly journals Behavioral and Antennal Responses of Tribolium confusum to Varronia globosa Essential Oil and Its Main Constituents: Perspective for Their Use as Repellent

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4393
Author(s):  
Cesar Auguste Badji ◽  
Jean Dorland ◽  
Lynda Kheloul ◽  
Dimitri Bréard ◽  
Pascal Richomme ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Confusum ◽  
High Dose ◽  
Synthetic Chemicals ◽  
Stored Product Pest ◽  
Indigenous Plant ◽  
Main Components ◽  
Electroantennogram Recordings ◽  
The Individual ◽  
Main Component

Essential oils of aromatic plants represent an alternative to classical pest control with synthetic chemicals. They are especially promising for the alternative control of stored product pest insects. Here, we tested behavioral and electrophysiological responses of the stored product pest Tribolium confusum, to the essential oil of a Brazilian indigenous plant, Varronia globosa, collected in the Caatinga ecosystem. We analyzed the essential oil by GC-MS, tested the effects of the entire oil and its major components on the behavior of individual beetles in a four-way olfactometer, and investigated responses to these stimuli in electroantennogram recordings (EAG). We could identify 25 constituents in the essential oil of V. globosa, with anethole, caryophyllene and spathulenole as main components. The oil and its main component anethole had repellent effects already at low doses, whereas caryophyllene had only a repellent effect at a high dose. In addition, the essential oil abolished the attractive effect of the T. confusum aggregation pheromone. EAG recordings revealed dose-dependent responses to the individual components and increasing responses to the blend and even more to the entire oil. Our study reveals the potential of anethole and the essential oil of V. globosa in the management of stored product pests.

Sign in / Sign up

Export Citation Format

Share Document