scholarly journals Tight Junction Protein 1a regulates pigment cell organisation during zebrafish colour patterning

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Andrey Fadeev ◽  
Jana Krauss ◽  
Hans Georg Frohnhöfer ◽  
Uwe Irion ◽  
Christiane Nüsslein-Volhard
Keyword(s):  
Tight Junction ◽  
Cell Shape ◽  
Pigment Cell ◽  
Tight Junction Protein ◽  
Pigment Cells ◽  
Colour Pattern ◽  
Junction Protein ◽  
Cell Shape Changes ◽  
Crucial Part ◽  
Shape Changes

Zebrafish display a prominent pattern of alternating dark and light stripes generated by the precise positioning of pigment cells in the skin. This arrangement is the result of coordinated cell movements, cell shape changes, and the organisation of pigment cells during metamorphosis. Iridophores play a crucial part in this process by switching between the dense form of the light stripes and the loose form of the dark stripes. Adult schachbrett (sbr) mutants exhibit delayed changes in iridophore shape and organisation caused by truncations in Tight Junction Protein 1a (ZO-1a). In sbr mutants, the dark stripes are interrupted by dense iridophores invading as coherent sheets. Immuno-labelling and chimeric analyses indicate that Tjp1a is expressed in dense iridophores but down-regulated in the loose form. Tjp1a is a novel regulator of cell shape changes during colour pattern formation and the first cytoplasmic protein implicated in this process.

scholarly journals Author response: Tight Junction Protein 1a regulates pigment cell organisation during zebrafish colour patterning

2015 ◽  
Author(s):  
Andrey Fadeev ◽  
Jana Krauss ◽  
Hans Georg Frohnhöfer ◽  
Uwe Irion ◽  
Christiane Nüsslein-Volhard
Keyword(s):  
Tight Junction ◽  
Pigment Cell ◽  
Tight Junction Protein ◽  
Author Response ◽  
Junction Protein

scholarly journals A genetic screen for temperature-sensitive morphogenesis-defectiveCaenorhabditis elegansmutants

2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Molly C Jud ◽  
Josh Lowry ◽  
Thalia Padilla ◽  
Erin Clifford ◽  
Yuqi Yang ◽  
...  
Keyword(s):  
Cell Shape ◽  
The Body ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Fundamental Biological Process ◽  
Embryonic Morphogenesis ◽  
Cell Shape Changes ◽  
Development Temperature ◽  
Multicellular Organisms ◽  
Shape Changes

AbstractMorphogenesis involves coordinated cell migrations and cell shape changes that generate tissues and organs, and organize the body plan. Cell adhesion and the cytoskeleton are important for executing morphogenesis, but their regulation remains poorly understood. As genes required for embryonic morphogenesis may have earlier roles in development, temperature-sensitive embryonic-lethal mutations are useful tools for investigating this process. From a collection of ∼200 such Caenorhabditis elegans mutants, we have identified 17 that have highly penetrant embryonic morphogenesis defects after upshifts from the permissive to the restrictive temperature, just prior to the cell shape changes that mediate elongation of the ovoid embryo into a vermiform larva. Using whole genome sequencing, we identified the causal mutations in seven affected genes. These include three genes that have roles in producing the extracellular matrix, which is known to affect the morphogenesis of epithelial tissues in multicellular organisms: the rib-1 and rib-2 genes encode glycosyltransferases, and the emb-9 gene encodes a collagen subunit. We also used live imaging to characterize epidermal cell shape dynamics in one mutant, or1219ts, and observed cell elongation defects during dorsal intercalation and ventral enclosure that may be responsible for the body elongation defects. These results indicate that our screen has identified factors that influence morphogenesis and provides a platform for advancing our understanding of this fundamental biological process.

scholarly journals Polycystin-2 Activity Is Controlled by Transcriptional Coactivator with PDZ Binding Motif and PALS1-associated Tight Junction Protein

2010 ◽  
Vol 285 (44) ◽  
pp. 33584-33588 ◽  
Author(s):  
Kerstin Duning ◽  
Deike Rosenbusch ◽  
Marc A. Schlüter ◽  
Yuemin Tian ◽  
Karl Kunzelmann ◽  
...  
Keyword(s):  
Tight Junction ◽  
Tight Junction Protein ◽  
Binding Motif ◽  
Transcriptional Coactivator ◽  
Junction Protein
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