scholarly journals An updated assessment ofSymbiodiniumspp. that associate with common scleractinian corals from Moorea (French Polynesia) reveals high diversity among background symbionts and a novel finding of clade B

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2856 ◽  
Author(s):  
Héloïse Rouzé ◽  
Gaël J. Lecellier ◽  
Denis Saulnier ◽  
Serge Planes ◽  
Yannick Gueguen ◽  
...  
Keyword(s):  
Coral Species ◽  
French Polynesia ◽  
Molecular Tools ◽  
Quantitative Real Time Pcr ◽  
Algal Symbionts ◽  
Clade B ◽  
Highly Sensitive ◽  
Low Levels ◽  
First Time ◽  
High Diversity

The adaptative bleaching hypothesis (ABH) states that, depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to “switch” or “shuffle” their algal symbionts), coral bleaching can lead to a change in the composition of their associatedSymbiodiniumcommunity and, thus, contribute to the coral’s overall survival. In order to determine the flexibility of corals, molecular tools are required to provide accurate species delineations and to detect low levels of coral-associatedSymbiodinium. Here, we used highly sensitive quantitative (real-time) PCR (qPCR) technology to analyse five common coral species from Moorea (French Polynesia), previously screened using only traditional molecular methods, to assess the presence of low-abundance (background)Symbiodiniumspp. Similar to other studies, each coral species exhibited a strong specificity to a particular clade, irrespective of the environment. In addition, however, each of the five species harboured at least one additionalSymbiodiniumclade, among clades A–D, at background levels. Unexpectedly, and for the first time in French Polynesia, clade B was detected as a coral symbiont. These results increase the number of known coral-Symbiodiniumassociations from corals found in French Polynesia, and likely indicate an underestimation of the ability of the corals in this region to associate with and/or “shuffle” differentSymbiodiniumclades. Altogether our data suggest that corals from French Polynesia may favor a trade-off between optimizing symbioses with a specificSymbiodiniumclade(s), maintaining associations with particular background clades that may play a role in the ability of corals to respond to environmental change.

scholarly journals An updated assessment of Symbiodinium that associate with common scleractinian corals from Moorea (French Polynesia) reveals high diversity among background symbionts and a novel finding of clade B.

2016 ◽  
Author(s):  
Héloïse Rouzé ◽  
Gaël J Lecellier ◽  
Denis Saulnier ◽  
Serge Planes ◽  
Yannick Gueguen ◽  
...  
Keyword(s):  
Coral Species ◽  
French Polynesia ◽  
Molecular Tools ◽  
Quantitative Real Time Pcr ◽  
Algal Symbionts ◽  
Clade B ◽  
Highly Sensitive ◽  
Low Levels ◽  
First Time ◽  
High Diversity

The adaptative bleaching hypothesis (ABH) states that depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to “switch” or “shuffle” their algal symbionts), coral bleaching can lead to a change in the composition of their associated Symbiodinium community, and, thus, contribute to the coral’s overall survival. In order to determine the flexibility of corals, molecular tools are required to provide accurate species delineations, and to detect low levels of coral-associated Symbiodinium. Here, we used highly sensitive quantitative (real-time) PCR (qPCR) technology to analyse five common coral species from Moorea (French Polynesia), previously screened using only traditional conventional molecular methods, to assess the presence of low-abundance (background) Symbiodinium. Similar to other studies, each coral species exhibited a strong specificity to a particular clade, irrespective of the environment. In addition, however, each of the five species harboured at least one additional Symbiodinium clade, among clades A-D, at background levels. Unexpectedly, and for the first time in French Polynesia, clade B was detected as a coral symbiont. These results increase the number of known coral-Symbiodinium associations from corals found in French Polynesia, and likely indicate an underestimation of the ability of the corals in this region to associate with and/or “shuffle” different Symbiodinium clades. Altogether our data suggest that corals from French Polynesia may manage a trade-off between optimizing symbioses with a specific Symbiodinium clade(s), and maintaining associations with particular background clades that may play a role in the ability of corals to respond to environmental change.

scholarly journals An updated assessment of Symbiodinium that associate with common scleractinian corals from Moorea (French Polynesia) reveals high diversity among background symbionts and a novel finding of clade B.

2016 ◽  
Author(s):  
Héloïse Rouzé ◽  
Gaël J Lecellier ◽  
Denis Saulnier ◽  
Serge Planes ◽  
Yannick Gueguen ◽  
...  
Keyword(s):  
Coral Species ◽  
French Polynesia ◽  
Molecular Tools ◽  
Quantitative Real Time Pcr ◽  
Algal Symbionts ◽  
Clade B ◽  
Highly Sensitive ◽  
Low Levels ◽  
First Time ◽  
High Diversity

The adaptative bleaching hypothesis (ABH) states that depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to “switch” or “shuffle” their algal symbionts), coral bleaching can lead to a change in the composition of their associated Symbiodinium community, and, thus, contribute to the coral’s overall survival. In order to determine the flexibility of corals, molecular tools are required to provide accurate species delineations, and to detect low levels of coral-associated Symbiodinium. Here, we used highly sensitive quantitative (real-time) PCR (qPCR) technology to analyse five common coral species from Moorea (French Polynesia), previously screened using only traditional conventional molecular methods, to assess the presence of low-abundance (background) Symbiodinium. Similar to other studies, each coral species exhibited a strong specificity to a particular clade, irrespective of the environment. In addition, however, each of the five species harboured at least one additional Symbiodinium clade, among clades A-D, at background levels. Unexpectedly, and for the first time in French Polynesia, clade B was detected as a coral symbiont. These results increase the number of known coral-Symbiodinium associations from corals found in French Polynesia, and likely indicate an underestimation of the ability of the corals in this region to associate with and/or “shuffle” different Symbiodinium clades. Altogether our data suggest that corals from French Polynesia may manage a trade-off between optimizing symbioses with a specific Symbiodinium clade(s), and maintaining associations with particular background clades that may play a role in the ability of corals to respond to environmental change.

scholarly journals Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Nadja Wilhelm ◽  
Nadja Perle ◽  
Robert Simmoteit ◽  
Christian Schlensak ◽  
Hans P. Wendel ◽  
...  
Keyword(s):  
Surgical Instruments ◽  
Surgical Instrument ◽  
Quantitative Real Time Pcr ◽  
Significant Extent ◽  
Cleaning Efficiency ◽  
Established Method ◽  
Highly Sensitive ◽  
First Time ◽  
Test Objects

Surgical instruments are often strongly contaminated with patients’ blood and tissues, possibly containing pathogens. The reuse of contaminated instruments without adequate cleaning and sterilization can cause postoperative inflammation and the transmission of infectious diseases from one patient to another. Thus, based on the stringent sterility requirements, the development of highly efficient, validated cleaning processes is necessary. Here, we use for the first time synthetic single-stranded DNA (ssDNA_ODN), which does not appear in nature, as a test soiling to evaluate the cleaning efficiency of routine washing processes. Stainless steel test objects were coated with a certain amount of ssDNA_ODN. After cleaning, the amount of residual ssDNA_ODN on the test objects was determined using quantitative real-time PCR. The established method is highly specific and sensitive, with a detection limit of 20 fg, and enables the determination of the cleaning efficiency of medical cleaning processes under different conditions to obtain optimal settings for the effective cleaning and sterilization of instruments. The use of this highly sensitive method for the validation of cleaning processes can prevent, to a significant extent, the insufficient cleaning of surgical instruments and thus the transmission of pathogens to patients.

More than local adaptation: high diversity of response to seawater acidification in seven coral species from the same assemblage in French Polynesia

2021 ◽  
pp. 1-9
Author(s):  
Mathilde Godefroid ◽  
Robin Arçuby ◽  
Yann Lacube ◽  
Benoit Espiau ◽  
Sam Dupont ◽  
...  
Keyword(s):  
Local Adaptation ◽  
Coral Species ◽  
French Polynesia ◽  
Coral Community ◽  
Tissue Necrosis ◽  
Calcification Rate ◽  
Seawater Acidification ◽  
Acropora Hyacinthus ◽  
Species Specific ◽  
High Diversity

Abstract Responses of corals to seawater acidification have been extensively studied. Sensitivity varies widely between species, highlighting the need to avoid extrapolation from one to another to get an accurate understanding of coral community responses. We tested the responses of seven coral species (Acropora cytherea, Acropora hyacinthus, Acropora pulchra, Leptastrea pruinosa, Montipora grisea, Pavona cactus, Pocillopora verrucosa) from the Mo'orea lagoon to a 48-day exposure to three pH scenarios (pH 7.95, 7.7 and 7.3). Tissue necrosis, mortality, growth rates, photophysiological performances and colour index were recorded. Few significant differences were noted between pH 7.95 and 7.7, but species-specific responses were observed at pH 7.3. While our data do not allow identification of the mechanisms behind this diversity in response between species inhabiting the same environment, it can exclude several hypotheses such as local adaptation, skeletal type, corallum morphology or calcification rate as sole factors determining coral sensitivity to pH.

First record of the superfamily Proctotrupoidea from French Polynesia (Hymenoptera)

2020 ◽  
Vol 125 (4) ◽  
pp. 404-406
Author(s):  
Thibault Ramage
Keyword(s):  
First Record ◽  
French Polynesia ◽  
Society Islands ◽  
First Time

A Helorid wasp, Helorus ruficornis Förster, 1856, is reported for the first time on Tahiti (Society Islands), which is also the first record of both Proctotrupoidea and family Heloridae in French Polynesia. The potential hosts of Helorus ruficornis in French Polynesia are discussed.

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