Phytochemical Characterization of Couch Grass (Cynodon dactylon) and Its Growth Promoting Potential on the Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

2016 ◽  
Vol 14 (2) ◽  
pp. 1-24 ◽  
Author(s):  
Kandaswamy Dhanalakshmi ◽  
Periyakali Bhavan ◽  
Gopalan Rajkumar ◽  
Virumandi Nathiya ◽  
Veeran Srinivasan ◽  
...  
Heliyon ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. e05898
Author(s):  
Tipsuda Thongbuakaew ◽  
Chanudporn Sumpownon ◽  
Attakorn Engsusophon ◽  
Napamanee Kornthong ◽  
Charoonroj Chotwiwatthanakun ◽  
...  

2021 ◽  
Vol 42 (1) ◽  
pp. 14-23
Author(s):  
B.B. Patnaik ◽  
◽  
S. Baliarsingh ◽  
S. Sahoo ◽  
J.M. Chung ◽  
...  

Aim: Identification of full-length ORF of hemocyanin subunit-1 (Mr_HC_1) from the hepatopancreas transcriptome of freshwater prawn, Macrobrachium rosenbergii infected with Vibrio harveyi and characterization of its sequence and structure by in silico tools and softwares. Methodology: Illumina HiSeq and de novo assembled unigenes were scanned against PANM-DB to screen Mr_HC_1. FGENESH gene prediction and SMART programs were used to predict the ORF region. Subsequently, Clustal X2 and MEGA in-silico tools were used to understand the sequence relatedness and evolutionary status of Mr_HC_1. Structural prediction was performed by SWISS-MODEL and Ramachandran plot modeling programs Results: The full-length ORF was 1983 bp in length encoding a polypeptide of 661 amino acid residues. Mr_HC_1 showed a putative signal peptide of 21 amino acid residues at the N-terminus and three hemocyanin domains. Homology analysis of Mr_HC_1 amino acid sequence confirms maximum identity to M. nipponense hemocyanin subunit-1 (Mn_HC_1). Phylogenetic analysis showed that Mr_HC_1 is more closely related to the hemocyanin γ-type subunit of freshwater shrimps. Homology modeling of Mr_HC_1 showed homo-hexameric protein containing 12 copper ions. With a QMEAN score of -3.33 and model-template sequence identity of 59.15%, the predicted model of Mr_HC_1 is convincing Interpretation: This study characterizes the hemocyanin γ-type subunit protein of freshwater prawn, M. rosenbergii for future studies on host defense mechanisms.


2008 ◽  
Vol 10 (5) ◽  
pp. 1473-1475 ◽  
Author(s):  
Shripathi Bhat ◽  
Alok Patel ◽  
Paramananda Das ◽  
Prem K. Meher ◽  
Bidu R. Pillai ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document