Genetic Diversity Study of Locally Isolated Bacillus thuringiensis Strains from Kuwait Using Random Amplified Polymorphic DNA Analysis

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Neis genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 8, 2009

Download Full-text

Isolation and Characterization of Microsatellite Loci of Clarias macrocephalus and Their Application to Genetic Diversity Study

Fisheries Science ◽

10.2331/fishsci.65.520 ◽

1999 ◽

Vol 65 (4) ◽

pp. 520-526 ◽

Cited By ~ 18

Author(s):

Uthairat Na-Nakorn ◽

Nobuhiko Taniguchi ◽

Estu Nugroho ◽

Shingo Seki ◽

Wongpathom Kamonrat

Keyword(s):

Genetic Diversity ◽

Microsatellite Loci ◽

Isolation And Characterization ◽

Clarias Macrocephalus ◽

Genetic Diversity Study ◽

Diversity Study

Download Full-text

Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

Nepal Journal of Science and Technology ◽

10.3126/njst.v13i2.7717 ◽

2013 ◽

Vol 13 (2) ◽

pp. 73-78

Author(s):

Jarina Joshsi ◽

Lumanti Manandhar ◽

Patima Shrestha ◽

Rani Gupta ◽

Rojlina Manadhar ◽

...

Keyword(s):

Genetic Diversity ◽

Rapd Markers ◽

Dna Analysis ◽

Genetic Characterization ◽

Random Amplified Polymorphic Dna ◽

Neighbor Joining ◽

German Shepherd ◽

Polymorphic Loci ◽

Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

Download Full-text