Studies of Influence of Ionic strengths of Cu (II) metal ion complex with Rubeanic acid by P H - metric technique

1. α-Mannosidase from the limpet, Patella vulgata, was purified nearly 150-fold, with 40% recovery. β-N-Acetylglucosaminidase was removed from the preparation by treatment with ethanol. The final product was virtually free from β-galactosidase. 2. Limpet α-mannosidase was assayed at pH3.5 and at this pH it was necessary to add Zn2+ for full activity. At pH5, the enzyme had the same activity in the presence or absence of added Zn2+. 3. On incubation at acid pH, the enzyme underwent reversible inactivation, which was prevented by adding Zn2+. 4. EDTA accelerated inactivation and the addition of Zn2+ at once restored activity. No other cation was found to reactivate the enzyme. 5. Cl- had an unspecific effect on hydrolysis by limpet α-mannosidase. It increased the rate of reaction with substrate. The anion did not prevent or reverse inactivation by EDTA. 6. It is concluded that α-mannosidase is a metalloenzyme or enzyme–metal ion complex, dissociable at the pH of activity, and that it requires Zn2+ specifically.

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Configurational Model of Metal Ion Complex Formation in Water Solutions

Physics and Chemistry of Liquids ◽

10.1080/00319100008030320 ◽

2000 ◽

Vol 38 (6) ◽

pp. 743-757 ◽

Cited By ~ 1

Author(s):

I. V. Stasyuk ◽

O. V. Velychko

Keyword(s):

Complex Formation ◽

Metal Ion ◽

Water Solutions ◽

Configurational Model ◽

Metal Ion Complex

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DNA binding domain of GAL4 forms a binuclear metal ion complex

Biochemistry ◽

10.1021/bi00464a019 ◽

1990 ◽

Vol 29 (12) ◽

pp. 3023-3029 ◽

Cited By ~ 35

Author(s):

Tao Pan ◽

Joseph E. Coleman

Keyword(s):

Dna Binding ◽

Metal Ion ◽

Binding Domain ◽

Dna Binding Domain ◽

Binuclear Metal ◽

Metal Ion Complex

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Selective Detection of Fe3+, F–, and Cysteine by a Novel Triazole-Linked Decaamine Derivative of Pillar[5]arene and Its Metal Ion Complex in Water

ACS Omega ◽

10.1021/acsomega.0c00595 ◽

2020 ◽

Vol 5 (11) ◽

pp. 6215-6220 ◽

Cited By ~ 1

Author(s):

Roymon Joseph

Keyword(s):

Metal Ion ◽

Selective Detection ◽

Metal Ion Complex

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Electron Spin Resonance Studies in Aqueous Solution: Titanium(III) in the pH Region 1.0–3.5

Canadian Journal of Chemistry ◽

10.1139/v74-425 ◽

1974 ◽

Vol 52 (16) ◽

pp. 2919-2922 ◽

Cited By ~ 9

Author(s):

Pavle Ilija Premović ◽

Paul Ronald West

Keyword(s):

Aqueous Solution ◽

Electron Spin Resonance ◽

Aqueous Solutions ◽

Electron Spin ◽

Metal Ion ◽

Active Species ◽

Single Line ◽

Spin Resonance ◽

Metal Ion Complex

In aqueous solutions of titanium(III) between pH 1.0 and 3.5 a single line e.s.r. signal is recorded with g = 1.9408 ± 0.001 and linewidth 95 ± 3 G. Evidence is presented in support of a hydrolyzed metal ion complex [Ti(OH)2(H2O)4]+ as the likely active species.

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