retinal ganglion cell
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2022 ◽  
Vol 12 (4) ◽  
pp. 788-793
Author(s):  
Lan Liu ◽  
Xinchao Cheng ◽  
Shaomin Li

This study investigated KLF7’s effect on sugar induced retinal ganglion cells (RGCs) biological activity. The RGCs cells divided into blank group (RA), high sugar group (RB), high sugar+NC group (RC) and high sugar+KLF7 group (RD) (transfected with KLF7 mimic) followed by analysis cell proliferation by MTT, cell apoptosis by flow cytometry and protein expression by western blot and ROS level. RB and RC group showed significantly reduced KLF7 mRNA and protein level compared to RA group (P < 0.05) without different between RB and RC group (P > 0.05). RD group had significantly increased LKF7 and Sirt1 protein expression (F = 113.3, P < 0.0, 01), reduced cell proliferation (P < 0.05) and increased RGCs apoptosis rate (P < 0.05) compared with RB and RC group. After 24 h, RB and RC group presented significantly higher ROS level (P < 0.05) which was reduced in RD group (P < 0.05). In conclusion, KLF7 can change sugar induced retinal ganglion cell biological activity and reduce the oxidative stress level.


Author(s):  
Michael L. Risner ◽  
Silvia Pasini ◽  
Nolan R. McGrady ◽  
David J. Calkins

AbstractThe BCL-2 (B-cell lymphoma-2) family of proteins contributes to mitochondrial-based apoptosis in models of neurodegeneration, including glaucomatous optic neuropathy (glaucoma), which degrades the retinal ganglion cell (RGC) axonal projection to the visual brain. Glaucoma is commonly associated with increased sensitivity to intraocular pressure (IOP) and involves a proximal program that leads to RGC dendritic pruning and a distal program that underlies axonopathy in the optic projection. While genetic deletion of the Bcl2-associated X protein (Bax-/-) prolongs RGC body survival in models of glaucoma and optic nerve trauma, axonopathy persists, thus raising the question of whether dendrites and the RGC light response are protected. Here, we used an inducible model of glaucoma in Bax-/- mice to determine if Bax contributes to RGC dendritic degeneration. We performed whole-cell recordings and dye filling in RGCs signaling light onset (αON-Sustained) and offset (αOFF-Sustained). We recovered RGC dendritic morphologies by confocal microscopy and analyzed dendritic arbor complexity and size. Additionally, we assessed RGC axon function by measuring anterograde axon transport of cholera toxin subunit B to the superior colliculus and behavioral spatial frequency threshold (i.e., spatial acuity). We found 1 month of IOP elevation did not cause significant RGC death in either WT or Bax-/- retinas. However, IOP elevation reduced dendritic arbor complexity of WT αON-Sustained and αOFF-Sustained RGCs. In the absence of Bax, αON- and αOFF-Sustained RGC dendritic arbors remained intact following IOP elevation. In addition to dendrites, neuroprotection by Bax-/- generalized to αON-and αOFF-Sustained RGC light- and current-evoked responses. Both anterograde axon transport and spatial acuity declined during IOP elevation in WT and Bax-/- mice. Collectively, our results indicate Bax contributes to RGC dendritic degeneration and distinguishes the proximal and distal neurodegenerative programs involved during the progression of glaucoma.


2022 ◽  
Vol 100 (S267) ◽  
Author(s):  
Rupali Vohra ◽  
Anna Luna Mølgaard Tams ◽  
Berta Sanz Morello ◽  
Zaynab Ahmad Mouhammad ◽  
Jens Hannibal ◽  
...  

2022 ◽  
Vol 100 (S267) ◽  
Author(s):  
Kristy Tatiana Rodríguez Ramírez ◽  
Manuel Vidal‐Sanz ◽  
Marta Agudo‐Barriuso

2022 ◽  
Vol 100 (S267) ◽  
Author(s):  
Ehtesham Shamsher ◽  
Li Guo ◽  
Benjamin M. Davis ◽  
Vy Luong ◽  
Nivedita Ravindran ◽  
...  

2022 ◽  
Vol 17 (3) ◽  
pp. 690
Author(s):  
RaoulTorero Ibad ◽  
KennethL Moya ◽  
Nicole Quenech’du ◽  
Alain Prochiantz

2022 ◽  
Vol 100 (S267) ◽  
Author(s):  
María Josefa González Riquelme ◽  
Fernando Lucas‐Ruiz ◽  
Raquel Boia ◽  
Ana Raquel Santiago ◽  
Manuel Vidal‐Sanz ◽  
...  

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