Assessment of Anthropogenically Stressed Ecosystem of Port Waters Using Macrobenthic Community-Biotic Indices

Macrobenthic community being used as marine health indictor vis-à-vis to anthropogenic disturbance since their characteristic of life cycle (reproductive mode, sedentary, energy transfer, sensitive-resistance species, indicator organism etc.). Therefore, the present study was chosen in Vizag port waters have been subject to a variety of anthropogenic pressure in the last two decades. The purpose of this study was to assess the ecological status of the port environment through biotic indices like AZTI 's Marine Biotic Index (AMBI) and Multivariate-AMBI Index (M-AMBI) which have been successfully validated geographically and anthropogenically stressed habitats. The result revealed that the inner harbor (IHC, Iron ore Q1 & Iron ore Q7) stations are heavily disturbed while the outer harbor fall between undisturbed and moderately disturbed status. It was observed that the reduced tidal action inside the harbor determines the health status indicating that the Iron ore transporting areas are at high risk with respect to benthic population.

Cross-Validation Indicates Predictive Models May Provide an Alternative to Indicator Organism Monitoring for Evaluating Pathogen Presence in Southwestern US Agricultural Water

Pathogen contamination of agricultural water has been identified as a probable cause of recalls and outbreaks. However, variability in pathogen presence and concentration complicates the reliable identification of agricultural water at elevated risk of pathogen presence. In this study, we collected data on the presence of Salmonella and genetic markers for enterohemorrhagic E. coli (EHEC; PCR-based detection of stx and eaeA) in southwestern US canal water, which is used as agricultural water for produce. We developed and assessed the accuracy of models to predict the likelihood of pathogen contamination of southwestern US canal water. Based on 169 samples from 60 surface water canals (each sampled 1–3 times), 36% (60/169) and 21% (36/169) of samples were positive for Salmonella presence and EHEC markers, respectively. Water quality parameters (e.g., generic E. coli level, turbidity), surrounding land-use (e.g., natural cover, cropland cover), weather conditions (e.g., temperature), and sampling site characteristics (e.g., canal type) data were collected as predictor variables. Separate conditional forest models were trained for Salmonella isolation and EHEC marker detection, and cross-validated to assess predictive performance. For Salmonella, turbidity, day of year, generic E. coli level, and % natural cover in a 500–1,000 ft (~150–300 m) buffer around the sampling site were the top 4 predictors identified by the conditional forest model. For EHEC markers, generic E. coli level, day of year, % natural cover in a 250–500 ft (~75–150 m) buffer, and % natural cover in a 500–1,000 ft (~150–300 m) buffer were the top 4 predictors. Predictive performance measures (e.g., area under the curve [AUC]) indicated predictive modeling shows potential as an alternative method for assessing the likelihood of pathogen presence in agricultural water. Secondary conditional forest models with generic E. coli level excluded as a predictor showed < 0.01 difference in AUC as compared to the AUC values for the original models (i.e., with generic E. coli level included as a predictor) for both Salmonella (AUC = 0.84) and EHEC markers (AUC = 0.92). Our data suggests models that do not require the inclusion of microbiological data (e.g., indicator organism) show promise for real-time prediction of pathogen contamination of agricultural water (e.g., in surface water canals).