fractional precipitation
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scholarly journals Simple, inexpensive and RNase-free purification of plasmid DNA by fractional precipitation with isopropanol

BioTechniques ◽  
2021 ◽  
Author(s):  
Viiu Paalme ◽  
Mart Speek
Keyword(s):  
Quality Control ◽  
Column Chromatography ◽  
Plasmid Dna ◽  
Fractional Precipitation ◽  
Alkaline Lysis ◽  
Bacterial Extract ◽  
Total Rna ◽  
Spin Column ◽  
Commercial Kits

We present a modified alkaline lysis method for purification of plasmid DNA (pDNA) from bacterial extract using fractional precipitation with isopropanol (FPI). This method includes two successive precipitations with 0.33 and 0.36 volumes of isopropanol and separates pDNA from total RNA and most of the lipopolysaccharides. Using different quality control tests, we demonstrate that plasmids purified with FPI show superior quality compared to plasmids prepared with commercial kits based on spin-column chromatography.

Chemical structure and inhibition on α-glucosidase of polysaccharides from corn silk by fractional precipitation

2021 ◽  
Vol 252 ◽  
pp. 117185
Author(s):  
Yanan Jia ◽  
Zihan Xue ◽  
Yajie Wang ◽  
Yangpeng Lu ◽  
Ruilin Li ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Fractional Precipitation ◽  
Corn Silk

Recovery of Fe and Al from red mud by a novel fractional precipitation process

2020 ◽  
Vol 27 (13) ◽  
pp. 14642-14653
Author(s):  
Fengqin Yu ◽  
Lin Huangfu ◽  
Chao Wang ◽  
Changming Li ◽  
Jian Yu ◽  
...  
Keyword(s):  
Red Mud ◽  
Precipitation Process ◽  
Fractional Precipitation
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