proteinase production
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2021 ◽  
Vol 25 (2) ◽  
pp. 261-274
Author(s):  
Chi Zhang ◽  
Guowei Shu ◽  
Ni Lei ◽  
Fangfang Cheng ◽  
Wenhui Li ◽  
...  

Abstract Cell envelope proteases (CEPs) can break down milk protein into peptides with different functions, which are of great benefit to human health. Therefore, the high-yield CEPs of Lactobacillus plantarum have the potential to produce functional dairy products. In previous experiments, we found that Na2HPO4, inulin, casein peptone and leucine have significant effects on CEP production by Lactobacillus plantarum LP69. So we proceeded to optimize the composition of the CEP-producing culture medium of L. plantarum through Box-Behnken design and response surface methodology. The protease activity, protein content and specific activity of CEPs produced by L. plantarum by inulin (0.2, 0.3, 0.4 %), casein peptone (0.4, 0.6, 0.8 %), Na2HPO4 (0.50, 0.52, 0.54 %) and leucine (14, 16, 18 mg/L) were evaluated. The optimal ratio of medium is 0.4 % inulin, 0.66 % casein peptone, 0.5 % Na2HPO4 and 14.04 mg/L Leucine. The final enzyme activity reached (24.46±0.81) U/mL, and the specific activity reached (1.41±0.46) U/mg.







2015 ◽  
Vol 181 (3-4) ◽  
pp. 217-224 ◽  
Author(s):  
Melyssa Negri ◽  
Sónia Silva ◽  
Isis Regina Grenier Capoci ◽  
Joana Azeredo ◽  
Mariana Henriques


2013 ◽  
Vol 12 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Rodnei Dennis Rossoni ◽  
Júnia Oliveira Barbosa ◽  
Simone Furgeri Godinho Vilela ◽  
Jéssica Diane dos Santos ◽  
Antonio Olavo Cardoso Jorge ◽  
...  


2013 ◽  
Vol 46 (3) ◽  
pp. 340-342 ◽  
Author(s):  
Antonella Souza Mattei ◽  
Sydney Hartz Alves ◽  
Cecilia Bittencourt Severo ◽  
Luciana da Silva Guazzelli ◽  
Flavio de Mattos Oliveira ◽  
...  


2012 ◽  
Vol 80 (1) ◽  
pp. 168-178 ◽  
Author(s):  
Mette H. Nicolaisen ◽  
Jakob Worm ◽  
Niels O.G. Jørgensen ◽  
Mathias Middelboe ◽  
Ole Nybroe


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