secreted aspartyl proteinase
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2021 ◽  
Vol 7 (12) ◽  
pp. 999
Author(s):  
Manisha Shukla ◽  
Pankaj Chandley ◽  
Harsimran Kaur ◽  
Anup K. Ghosh ◽  
Shivaprakash M. Rudramurthy ◽  
...  

Systemic candidiasis is the fourth most common bloodstream infection in ICU patients worldwide. Although C. albicans is a predominant species causing systemic candidiasis, infections caused by non-albicans Candida (NAC) species are increasingly becoming more prevalent globally along with the emergence of drug resistance. The diagnosis of systemic candidiasis is difficult due to the absence of significant clinical symptoms in patients. We investigated the diagnostic potential of recombinant secreted aspartyl proteinase 2 (rSap2) from C. parapsilosis for the detection of Candida infection. The rSap2 protein was successfully cloned, expressed and purified using Ni-NTA chromatography under denaturing conditions using an E. coli-based prokaryotic expression system, and refolded using a multi-step dialysis procedure. Structural analysis by CD and FTIR spectroscopy revealed the refolded protein to be in its near native conformation. Immunogenicity analysis demonstrated the rSap2 protein to be highly immunogenic as evident from significantly high titers of Sap2-specific antibodies in antigen immunized Balb/c mice, compared to sham-immunized controls. The diagnostic potential of rSap2 protein was evaluated using immunoblotting and ELISA assays using proven candidiasis patient serum and controls. Immunoblotting results indicate that reactivity to rSap2 was specific to candidiasis patient sera with no cross reactivity observed in healthy controls. Increased levels of anti-Sap2-specific Ig, IgG and IgM antibodies were observed in candidiasis patients compared to controls and was similar in sensitivity obtained when whole Candida was used as coating antigen. In summary, the rSap2 protein from C. parapsilosis has the potential to be used in the diagnosis of systemic candidiasis, providing a rapid, convenient, accurate and cost-effective strategy.



2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Josidel Conceição Oliver ◽  
Luca Laghi ◽  
Carola Parolin ◽  
Claudio Foschi ◽  
Antonella Marangoni ◽  
...  

Abstract Candida species are the most common cause of opportunistic fungal infections. Rapid identification and novel approaches for the characterization of these fungi are of great interest to improve the diagnosis and the knowledge about their pathogenic properties. This study aimed to characterize clinical isolates of Candida spp. by proteomics (MALDI-TOF MS) and metabolomics (1H-NMR), and to correlate their metabolic profiles with Candida species, source of infection and different virulence associated parameters. In particular, 49 Candida strains from different sources (blood, n = 15; vagina, n = 18; respiratory tract, n = 16), belonging mainly to C. albicans complex (61%), C. glabrata (20%) and C. parapsilosis (12%) species were used. Several extracellular and intracellular metabolites showed significantly different concentrations among isolates recovered from different sources of infection, as well as among different Candida species. These metabolites were mainly related to the glycolysis or gluconeogenesis, tricarboxylic acid cycle, nucleic acid synthesis and amino acid and lipid metabolism. Moreover, we found specific metabolic fingerprints associated with the ability to form biofilm, the antifungal resistance (i.e. caspofungin and fluconazole) and the production of secreted aspartyl proteinase. In conclusion, 1H-NMR-based metabolomics can be useful to deepen Candida spp. virulence and pathogenicity properties.



2020 ◽  
Vol 25 (3) ◽  
pp. 316-324
Author(s):  
Kemal Bilgin ◽  
Asuman Birinci ◽  
Tuba Yıldırım

Introduction: In recent years, increasing infections due to fungi have drawn attention. Especially, Candida albicans is the most frequent infectious agent with its virulence factors. Furthermore, because C. albicans is a nosocomial infection agent, it can lead an increase in mortality and morbidity. In this study, it was aimed to genotype C. albicans strains that caused candidemia by molecular methods and to investigate one of the most important virulence factors, Secreted Aspartyl Proteinase 4 (SAP4). Materials and Methods: Our study included 50 C. albicans strains isolated from blood cultures. The isolates were identified both phenotypically and genotypically. Arbitrarily-Primed Polymerase Chain Reaction (AP-PCR) and Pulsed Field Gel ElectrophoresisElectrophoretic Karyotype Analysis (PFGE-EK) methods were used for molecular genotyping of the strains. Furthermore, the presence of SAP4 a virulence factor for C. albicans was investigated by polymerase chain reaction (PCR). Results: The 50 C. albicans isolates included in our study were separated to 26 genotypes by AP-PCR, 41 genotypes by PFGE-EK, and SAP4 was detected in 49 of the 50 isolates. Conclusion: In conclusion, dominant genotypes among the C. albicans isolates were not detected. Results of both molecular typing methods revealed that C. albicans isolates were generally endogenous. Furthermore, SAP4 gene was detected in 98% of the isolates. A considerable presence of this gene in blood sample isolates suggests that it contributes to systemic infections. More elaborative studies on the function of SAP4 gene in endogenous infections of C. albicans may contribute to the prevention of these infections in the future.



2020 ◽  
Vol 88 (10) ◽  
Author(s):  
Manisha Shukla ◽  
Soma Rohatgi

ABSTRACT The rising incidence of non-albicans Candida species globally, along with the emergence of drug resistance, is a cause for concern. This study investigated the protective efficacy of secreted aspartyl proteinase 2 (Sap2) in systemic C. tropicalis infection. Vaccination with recombinant Sap2 (rSap2) protein from C. parapsilosis enhanced survival of mice compared to rSap2 vaccinations from C. albicans (P = 0.02), C. tropicalis (P = 0.06), and sham immunization (P = 0.04). Compared to sham-immunized mice, the fungal CFU number was significantly reduced in organs of Sap2-parapsilosis-immunized mice. Histopathologically, increased neutrophilic recruitment was observed in Sap2-parapsilosis- and Sap2-tropicalis-immunized mice. Among different rSap2 proteins, Sap2-parapsilosis vaccination induced increased titers of Sap2-specific Ig, IgG, and IgM antibodies, which could bind whole fungus. Between different groups, sera from Sap2-parapsilosis-vaccinated mice exhibited increased C. tropicalis biofilm inhibition ability in vitro and enhanced neutrophil-mediated fungal killing. Passive transfer of anti-Sap2-parapsilosis immune serum in naive mice significantly reduced fungal burdens compared to those in mice receiving anti-sham immune serum. Higher numbers of plasma cells and Candida-binding B cells in Sap2-vaccinated mice suggest a role of B cells during early stages of Sap2-mediated immune response. Additionally, increased levels of Th1/Th2/Th17 cytokines observed in Sap2-parapsilosis-vaccinated mice indicate immunomodulatory properties of Sap2. Epitope analysis performed using identified B-cell epitopes provides a basis to understand differences in immunogenicity observed among Sap2-antigens and can aid the development of a multivalent or multiepitope anti-Candida vaccine(s). In summary, our results suggest that Sap2-parapsilosis vaccination can improve mouse survival during C. tropicalis infection by inducing both humoral and cellular immunity, and higher titers of Sap2-induced antibodies are beneficial during systemic candidiasis.



2019 ◽  
Vol 46 (6) ◽  
pp. 6147-6154 ◽  
Author(s):  
Carolina do Rosário Esteves Guimarães ◽  
Humberto Fonseca de Freitas ◽  
Tânia Fraga Barros


2019 ◽  
Vol 1153 ◽  
pp. 012141
Author(s):  
Alfin Titian Permata ◽  
Afiono Agung Prasetyo ◽  
Ruben Dharmawan


2018 ◽  
Vol 12 (9) ◽  
pp. e0006806 ◽  
Author(s):  
Daniele Gonçalves Castilho ◽  
Alison Felipe Alencar Chaves ◽  
Marina Valente Navarro ◽  
Palloma Mendes Conceição ◽  
Karen Spadari Ferreira ◽  
...  




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