Isolation of nuclei from frozen human skeletal and cardiac muscle for single nucleus RNA and chromatin assays v2

This protocol was adapted for the isolation of single nuclei from frozen skeletal and cardiac muscle tissues for molecular characterization with the SNARE-seq2, sci-ATAC-seq, and snRNA-seq assays. References: (1) Preissl et al (2015). Circulation Research. Doi: 10.1161/CIRCRESAHA.115.306337 (2) Hocker et al (2021). Science Advances. Doi: 10.1126/sciadv.abf1444

Pathophysiology of Hypertension

Dr Irvine Page proposed the Mosaic Theory of Hypertension in the 1940s advocating that hypertension is the result of many factors that interact to raise blood pressure and cause end-organ damage. Over the years, Dr Page modified his paradigm, and new concepts regarding oxidative stress, inflammation, genetics, sodium homeostasis, and the microbiome have arisen that allow further refinements of the Mosaic Theory. A constant feature of this approach to understanding hypertension is that the various nodes are interdependent and that these almost certainly vary between experimental models and between individuals with hypertension. This review discusses these new concepts and provides an introduction to other reviews in this compendium of Circulation Research .

C. Menger’s theory of the value of money

The aim of the article is a complex presentation of C. Menger’s theory of the value of money. The main method was the content analysis of his little-known articles devoted both to monetary theory and to practical issues of monetary reform in Austria-Hungary. In accordance with existing classifications, Menger’s theory of money may be defined as evolutionary according to the criteria of origin, functional according to the criteria of the nature of value, and psychological according to the criteria of factors of value change. It has been demonstrated that Menger’s initial view of the source of the value of money in line with the substantial approach can be explained by combining it with an evolutionary approach to the origin of money and opposition to nominalism based on a rationalistic approach. Later, in the works of the 1890s, there is a shift towards functional approach, due, among other things, to the emergence of new economic phenomena. Menger’s denial of the mechanistic version of the quantitative theory of money as inconsistent with the basic assumptions of the analysis is shown. Determining the essence of money by its position in the national economy, formed by the subjective actions of people, creates the basis for a psychological explanation of the change in the value of money. The article analyzes the concepts of the inner and outer value of money introduced by Menger into science and the reasons for refusing to consider the idea of a constant outer value. Maintaining a stable inner value of money by regulating its quantity in circulation requires careful analytical and statistical efforts, and Menger warns against arbitrary active government intervention in the sphere of money circulation. Research prospects are seen in the planes of analyzing the influence of the approaches to the theory of money laid down by Menger on the subsequent theories of various schools and the phenomenon of the seeming duality of Menger’s approach as a factor of the bifurcation of the Austrian economic school.

Farmakologi Kardiovaskuler, Mekanisme & Aplikasi Klinis

Untuk mencapai maksud tersebut, maka buku Farmakologi Kardiovaskuler edisi ke 3, tetap disusun sebanyak 8 bab, dengan pembahasan dilakukan secara berurutan : ● Pengantar terapeutik● Mekanisme kerja ● Farmakokinetik● Efek samping dan interaksi obat● Kedokteran berbasis bukti● Aplikasi klinisPenambahan informasi diperoleh dari berbagai sumber penerbitan terkini sesuai dengan kebutuhan penerapan dalam praktek klinis sehari-hari, termasuk hasil penelitian penulis. Untuk itu, revisi dilakukan dengan menambah informasi yang relevan mulai dari bab 1 sampai bab 8, terutama pemahaman terbaru patobiologi, yang mengantarkan pada perkembangan penemuan obat, mekanisme kerja obat, farmakokinetik, hasil penelitian eksperimental dan klinis, yang disari dari berbagai jurnal internasional terkemuka di dunia, seperti: Nature, Science, Lancet, New England Journal of Medicine, Circulation, Circulation Research, Cellular and Molecular Life Sciences, Journal of the American College of Cardiology, Hypertension, Stroke, European Heart Journal, Arteriosclerosis, Thrombosis and Vascular Biology, Current Cardiology Review dan berbagai buku Farmakologi. Karena itu, buku ini ditujukan kepada seluruh mahasiswa kedokteran dan kesehatan, para dokter umum dan spesialis, apoteker dan insan kesehatan, untuk memberikan tambahan informasi pengetahuan dalam pengobatan yang rasional, khususnya di bidang farmakologi kardiovaskuler, sekaligus memberikan motivasi dan inspirasi dalam penelitian.