hydroxynitrile lyase
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2022 ◽  
pp. 105594
Author(s):  
Badipatla Vishnu Priya ◽  
D.H. Sreenivasa Rao ◽  
Rubina Gilani ◽  
Surabhi Lata ◽  
Nivedita Rai ◽  
...  

2020 ◽  
Vol 153 ◽  
pp. 112606
Author(s):  
R. Marlen Martínez-Casares ◽  
HerminiaI Pérez ◽  
Norberto Manjarrez ◽  
Myrna Solís-Oba ◽  
Lucia Ortega ◽  
...  

FEBS Journal ◽  
2020 ◽  
Author(s):  
Fumihiro Motojima ◽  
Atsushi Izumi ◽  
Aem Nuylert ◽  
Zhenyu Zhai ◽  
Mohammad Dadashipour ◽  
...  

Catalysts ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 899
Author(s):  
José Coloma ◽  
Tim Lugtenburg ◽  
Muhammad Afendi ◽  
Mattia Lazzarotto ◽  
Paula Bracco ◽  
...  

Arabidopsis thaliana hydroxynitrile lyase (AtHNL) catalyzes the selective synthesis of (R)-cyanohydrins. This enzyme is unstable under acidic conditions, therefore its immobilization is necessary for the synthesis of enantiopure cyanohydrins. EziG Opal is a controlled porosity glass material for the immobilization of His-tagged enzymes. The immobilization of His6-tagged AtHNL on EziG Opal was optimized for higher enzyme stability and tested for the synthesis of (R)-mandelonitrile in batch and continuous flow systems. AtHNL-EziG Opal achieved 95% of conversion after 30 min of reaction time in batch and it was recycled up to eight times with a final conversion of 80% and excellent enantioselectivity. The EziG Opal carrier catalyzed the racemic background reaction; however, the high enantioselectivity observed in the recycling study demonstrated that this was efficiently suppressed by using citrate/phosphate buffer saturated methyl-tert-butylether (MTBE) pH 5 as reaction medium. The continuous flow system achieved 96% of conversion and excellent enantioselectivity at 0.1 mL min−1. Lower conversion and enantioselectivity were observed at higher flow rates. The specific rate of AtHNL-EziG Opal in flow was 0.26 mol h−1 genzyme−1 at 0.1 mL min−1 and 96% of conversion whereas in batch, the immobilized enzyme displayed a specific rate of 0.51 mol h−1 genzyme−1 after 30 min of reaction time at a similar level of conversion. However, in terms of productivity the continuous flow system proved to be almost four times more productive than the batch approach, displaying a space-time-yield (STY) of 690 molproduct h−1 L−1 genzyme−1 compared to 187 molproduct h−1 L−1 genzyme−1 achieved with the batch system.


PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0235341
Author(s):  
Bryan J. Jones ◽  
Robert L. Evans ◽  
Nathan J. Mylrea ◽  
Debayan Chaudhury ◽  
Christine Luo ◽  
...  

ACS Catalysis ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 5757-5763
Author(s):  
Yu-Cong Zheng ◽  
Fu-Long Li ◽  
Zuming Lin ◽  
Guo-Qiang Lin ◽  
Ran Hong ◽  
...  
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