polyacrylamide electrophoresis
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2022 ◽  
Vol 2022 (1) ◽  
pp. pdb.prot103143
Author(s):  
Edward A. Greenfield

Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after sodium dodecyl sulfate (SDS)–polyacrylamide electrophoresis and thus it is simple to discriminate between true and false positives. Furthermore, the assay provides information regarding the molecular weight of the antigen.


2021 ◽  
Author(s):  
Reuben Anderson ◽  
Evelyn Sattlegger

Abstract Precast gels are made with plastic cassettes which usually are discarded after use. Here we describe how Criterion plastic gel cassettes can be re-used for making SDS-PAGE gels in-house.


2019 ◽  
Vol 7 (34) ◽  
pp. 5156-5160 ◽  
Author(s):  
Emerald R. Taylor ◽  
Silvia Cavuoto ◽  
David M. Beal ◽  
Sophie Caujolle ◽  
Adrian Podoleanu ◽  
...  

Gold nanoparticles, encapsulated within polyacrylamide electrophoresis gels, can discriminate between number of type of sulphur modifications in DNA strands.


2018 ◽  
Vol 55 (5) ◽  
pp. 1942-1947 ◽  
Author(s):  
Miguel Angel Duarte-Vázquez ◽  
Carlos Raúl García-Ugalde ◽  
Blanca Erika Álvarez ◽  
Laura Mariana Villegas ◽  
Blanca Estela García-Almendárez ◽  
...  

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