An improved acetic acid‐urea polyacrylamide electrophoresis method to evaluate bovine sperm protamines

The study presents the extraction of collagen, a product of high value, from fleshings form hides. After testing several collagen extraction procedures we have proposed the simple and effective method to extract collagen from collagen-containing wastes of the leather industry. The unified method is based on the extraction of collagen using acetic acid in the presence of EDTA and included two repeated extraction stages. Qualitative analysis of the collagen using the disk-electrophoresis method showed a different ratio of monomers, dimers and other proteins.

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The Roles of Acetic Acid and Methanol During Fixing and Staining Proteins in an SDS–Polyacrylamide Electrophoresis Gel

Methods in Molecular Biology - Protein Gel Detection and Imaging ◽

10.1007/978-1-4939-8745-0_2 ◽

2018 ◽

pp. 15-18 ◽

Cited By ~ 1

Author(s):

J. P. Dean Goldring

Keyword(s):

Acetic Acid ◽

Polyacrylamide Electrophoresis

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A novel serum: Electrophoresis method to prepare acellular corneal matrix as an artificial corneal scaffold

The International Journal of Artificial Organs ◽

10.1177/0391398819869941 ◽

2019 ◽

Vol 43 (2) ◽

pp. 127-136

Author(s):

Qing Li ◽

Cuicui Xie ◽

Hongmei Wang ◽

Fenghua Zhang ◽

Lanlan Mu

Keyword(s):

Acetic Acid ◽

Human Serum ◽

Glacial Acetic Acid ◽

Lamellar Keratoplasty ◽

Corneal Tissue ◽

Electrophoresis Method ◽

Good Biocompatibility ◽

Implant Test ◽

Subcutaneous Implant ◽

Better Than

Introduction: The aim of this study was to develop a novel decellularization method in order to obtain an ideal scaffold with good biocompatibility. Methods: The porcine corneas were treated with human serum for 5 days or serum-electrophoresis respectively. The electrophoresis (100 V/cm) was performed in sterilized buffer containing 40-mM tris base, 18-mM glacial acetic acid, and antibiotics for 1 h at 4°C. The properties of artificial corneal scaffolds were characterized by morphological and histological examinations. The biocompatibility and biological safety were examined by subcutaneous implant test and lamellar keratoplasty. Results and conclusions: The transparency and appearance of serum-electrophoresis acellular porcine corneal matrix were better than serum acellular porcine corneal matrix. DNA and α-gal in serum-electrophoresis acellular porcine corneal matrix were more efficiently removed than those in serum acellular porcine corneal matrix (p < 0.05). The subcutaneous and corneal implantation experiments showed serum-electrophoresis acellular porcine corneal matrix had better biocompatibility compared to serum acellular porcine corneal matrix (p < 0.01). This novel serum-electrophoresis decellularization method may be valuable for preparation of xenogenic corneal tissue for clinical application.

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Study on development of an analytical procedure to quantify glyphosate by capillary electrophoresis method and its application for beverage

Ministry of Science and Technology, Vietnam ◽

10.31276/vjst.63(11).58-64 ◽

2021 ◽

Vol 63 (11) ◽

pp. 58-64

Author(s):

Manh Huy Nguyen ◽

◽

Thi Ha Tran ◽

Minh Tuan Vu ◽

Thanh Dam Nguyen ◽

...

Keyword(s):

Acetic Acid ◽

Capillary Electrophoresis ◽

Analytical Procedure ◽

Ph Value ◽

Solid Phase ◽

Residue Analysis ◽

Background Electrolyte ◽

Capillary Electrophoresis Method ◽

Relative Standard ◽

Electrophoresis Method

Nowadays, the issue of fresh food, drinks, and residue analysis of toxic compounds in food and drinks is increasingly interested in society. In this study, an analytical procedure for quantitative analysis of the glyphosate residue in beverages such as tea infusion and beer was developed based on the capillary electrophoresis method with capacitively-coupled contactless conductivity detector combined with sample preparation using solid phase extraction technique. The analytical conditions optimised for capillary electrophoresis equipment included: histidine/acetic acid background electrolyte solution with histidine concentration of 1 mM and pH value was adjusted to 2.75 by acetic acid; a separation voltage was 20 kV was applied and a high voltage injection at 20 kV for 10 seconds was chosen. The optimised analytical procedure has resulted in a low detection limit of glyphosate (0.42 μg/l), the repeatability and reproducibility expressed by the relative standard deviation of the peak area and migration time were both less than 10%, a wide linear range, and the obtained recovery efficiency of glyphosate on different drinks were achieved to values ranging from 88.7 to 96.3%.

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Ectodesmata as Revealed By Freeze-Etch Preparations of Plant Epidermal Cell Walls

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072782 ◽

1973 ◽

Vol 31 ◽

pp. 468-469

Author(s):

N.C. Lyon ◽

W. C. Mueller

Keyword(s):

Electron Microscopy ◽

Acetic Acid ◽

Nitric Acid ◽

Oxalic Acid ◽

Light Microscopy ◽

Epidermal Cell ◽

Cell Walls ◽

Plant Viruses ◽

Plant Leaves ◽

Thin Sections

Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to saturation 2-3 g). Other workers have published electron micrographs of structures transversing the outer epidermal cell in thin sections of plant leaves that have been interpreted as ectodesmata. Such structures are evident following treatment with Hg++ or Ag+ salts and are only rarely observed by electron microscopy. If ectodesmata exist without such treatment, and are not artefacts, they would afford natural pathways of entry for applied foliar solutions and plant viruses.

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